Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltB and Shew_1033 are separated by 430 nucleotides Shew_1033 and Shew_1034 are separated by 173 nucleotides Shew_1034 and Shew_1035 are separated by 205 nucleotides
Shew_1032: gltB - glutamate synthase subunit alpha (RefSeq), at 1,190,911 to 1,195,359
gltB
Shew_1033: Shew_1033 - putative radical SAM protein (RefSeq), at 1,195,790 to 1,196,782
_1033
Shew_1034: Shew_1034 - Hpt protein (RefSeq), at 1,196,956 to 1,197,321
_1034
Shew_1035: Shew_1035 - LysR family transcriptional regulator (RefSeq), at 1,197,527 to 1,198,444
_1035
Position (kb)
1195
1196
1197 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1194.851 kb on + strand, within gltB at 1194.859 kb on - strand, within gltB at 1194.939 kb on - strand at 1194.984 kb on + strand at 1195.706 kb on + strand at 1195.860 kb on + strand at 1195.977 kb on + strand, within Shew_1033 at 1196.029 kb on + strand, within Shew_1033 at 1196.071 kb on + strand, within Shew_1033 at 1196.126 kb on - strand, within Shew_1033 at 1196.178 kb on + strand, within Shew_1033 at 1196.267 kb on - strand, within Shew_1033 at 1196.307 kb on + strand, within Shew_1033 at 1196.657 kb on - strand, within Shew_1033 at 1197.416 kb on + strand at 1197.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 1,194,851 + gltB Shew_1032 0.89 +0.2 1,194,859 - gltB Shew_1032 0.89 -0.0 1,194,939 - -1.5 1,194,984 + -0.6 1,195,706 + -1.4 1,195,860 + -0.2 1,195,977 + Shew_1033 0.19 -0.5 1,196,029 + Shew_1033 0.24 -0.4 1,196,071 + Shew_1033 0.28 -4.5 1,196,126 - Shew_1033 0.34 -2.8 1,196,178 + Shew_1033 0.39 -2.5 1,196,267 - Shew_1033 0.48 -0.9 1,196,307 + Shew_1033 0.52 -0.1 1,196,657 - Shew_1033 0.87 -0.5 1,197,416 + +0.2 1,197,452 + -4.7
Or see this region's nucleotide sequence