Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0732 and Shew_0733 are separated by 30 nucleotides Shew_0733 and Shew_0734 are separated by 237 nucleotides Shew_0734 and Shew_0735 are separated by 20 nucleotides
Shew_0732: Shew_0732 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 833,279 to 834,619
_0732
Shew_0733: Shew_0733 - hypothetical protein (RefSeq), at 834,650 to 835,405
_0733
Shew_0734: Shew_0734 - Fe-S metabolism associated SufE (RefSeq), at 835,643 to 836,095
_0734
Shew_0735: Shew_0735 - SufS subfamily cysteine desulfurase (RefSeq), at 836,116 to 837,327
_0735
Position (kb) 834
835
836 Strain fitness (log2 ratio) -1
0
1
2 at 833.776 kb on + strand, within Shew_0732 at 833.894 kb on - strand, within Shew_0732 at 834.052 kb on + strand, within Shew_0732 at 834.209 kb on - strand, within Shew_0732 at 834.209 kb on - strand, within Shew_0732 at 834.727 kb on - strand, within Shew_0733 at 834.786 kb on + strand, within Shew_0733 at 834.786 kb on + strand, within Shew_0733 at 834.794 kb on - strand, within Shew_0733 at 834.794 kb on - strand, within Shew_0733 at 835.380 kb on + strand at 835.542 kb on + strand at 835.550 kb on - strand at 836.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 833,776 + Shew_0732 0.37 -1.0 833,894 - Shew_0732 0.46 -0.3 834,052 + Shew_0732 0.58 +0.2 834,209 - Shew_0732 0.69 -0.8 834,209 - Shew_0732 0.69 -0.2 834,727 - Shew_0733 0.10 +0.5 834,786 + Shew_0733 0.18 +1.9 834,786 + Shew_0733 0.18 +0.4 834,794 - Shew_0733 0.19 -0.0 834,794 - Shew_0733 0.19 +0.5 835,380 + -0.9 835,542 + +0.3 835,550 - +1.0 836,082 - +0.2
Or see this region's nucleotide sequence