Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0499 and Shew_0500 are separated by 88 nucleotides Shew_0500 and Shew_0501 are separated by 202 nucleotides Shew_0501 and Shew_0502 are separated by 9 nucleotides
Shew_0499: Shew_0499 - Xaa-Pro aminopeptidase family enzyme (RefSeq), at 557,784 to 559,136
_0499
Shew_0500: Shew_0500 - hypothetical protein (RefSeq), at 559,225 to 560,241
_0500
Shew_0501: Shew_0501 - hypothetical protein (RefSeq), at 560,444 to 560,728
_0501
Shew_0502: Shew_0502 - hypothetical protein (RefSeq), at 560,738 to 561,265
_0502
Position (kb)
559
560
561 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 558.375 kb on - strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.411 kb on - strand, within Shew_0499 at 558.608 kb on + strand, within Shew_0499 at 558.679 kb on + strand, within Shew_0499 at 559.472 kb on + strand, within Shew_0500 at 559.980 kb on - strand, within Shew_0500 at 560.254 kb on + strand at 560.257 kb on - strand at 560.272 kb on - strand at 560.294 kb on + strand at 560.487 kb on + strand, within Shew_0501 at 560.497 kb on + strand, within Shew_0501 at 560.699 kb on + strand, within Shew_0501 at 560.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 558,375 - Shew_0499 0.44 -0.9 558,403 + Shew_0499 0.46 -2.4 558,403 + Shew_0499 0.46 -2.8 558,403 + Shew_0499 0.46 +0.4 558,411 - Shew_0499 0.46 +0.2 558,608 + Shew_0499 0.61 +0.1 558,679 + Shew_0499 0.66 -1.7 559,472 + Shew_0500 0.24 +1.2 559,980 - Shew_0500 0.74 +0.5 560,254 + +0.7 560,257 - -0.2 560,272 - +0.9 560,294 + +0.1 560,487 + Shew_0501 0.15 -0.3 560,497 + Shew_0501 0.19 +0.6 560,699 + Shew_0501 0.89 +0.5 560,707 - +0.4
Or see this region's nucleotide sequence