Experiment: m.b. perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3979 and Dshi_3980 are separated by 16 nucleotides Dshi_3980 and Dshi_3981 are separated by 2 nucleotides Dshi_3981 and Dshi_3982 overlap by 4 nucleotides
Dshi_3979: Dshi_3979 - hypothetical protein (RefSeq), at 38,371 to 39,180
_3979
Dshi_3980: Dshi_3980 - VirB8 family protein (RefSeq), at 39,197 to 39,850
_3980
Dshi_3981: Dshi_3981 - Conjugal transfer protein TrbG/VirB9/CagX (RefSeq), at 39,853 to 40,548
_3981
Dshi_3982: Dshi_3982 - conjugation TrbI family protein (RefSeq), at 40,545 to 41,924
_3982
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1
2 at 38.883 kb on + strand, within Dshi_3979 at 38.883 kb on + strand, within Dshi_3979 at 38.883 kb on + strand, within Dshi_3979 at 38.891 kb on - strand, within Dshi_3979 at 38.932 kb on - strand, within Dshi_3979 at 38.951 kb on + strand, within Dshi_3979 at 39.107 kb on - strand at 39.165 kb on + strand at 39.180 kb on + strand at 39.192 kb on + strand at 39.357 kb on + strand, within Dshi_3980 at 39.366 kb on + strand, within Dshi_3980 at 39.366 kb on + strand, within Dshi_3980 at 39.384 kb on + strand, within Dshi_3980 at 39.392 kb on - strand, within Dshi_3980 at 39.392 kb on - strand, within Dshi_3980 at 39.394 kb on + strand, within Dshi_3980 at 39.437 kb on - strand, within Dshi_3980 at 39.463 kb on - strand, within Dshi_3980 at 39.474 kb on + strand, within Dshi_3980 at 39.529 kb on - strand, within Dshi_3980 at 39.531 kb on + strand, within Dshi_3980 at 39.574 kb on - strand, within Dshi_3980 at 39.689 kb on + strand, within Dshi_3980 at 39.715 kb on + strand, within Dshi_3980 at 39.721 kb on + strand, within Dshi_3980 at 39.724 kb on + strand, within Dshi_3980 at 39.732 kb on - strand, within Dshi_3980 at 39.782 kb on + strand, within Dshi_3980 at 39.786 kb on + strand at 39.789 kb on - strand at 39.790 kb on - strand at 39.790 kb on - strand at 39.801 kb on + strand at 39.801 kb on + strand at 39.803 kb on - strand at 39.809 kb on - strand at 39.844 kb on + strand at 39.889 kb on - strand at 39.912 kb on - strand at 39.962 kb on + strand, within Dshi_3981 at 39.962 kb on + strand, within Dshi_3981 at 39.962 kb on + strand, within Dshi_3981 at 39.962 kb on + strand, within Dshi_3981 at 39.970 kb on - strand, within Dshi_3981 at 39.973 kb on - strand, within Dshi_3981 at 39.981 kb on - strand, within Dshi_3981 at 40.000 kb on - strand, within Dshi_3981 at 40.072 kb on - strand, within Dshi_3981 at 40.073 kb on + strand, within Dshi_3981 at 40.118 kb on + strand, within Dshi_3981 at 40.161 kb on - strand, within Dshi_3981 at 40.268 kb on - strand, within Dshi_3981 at 40.322 kb on - strand, within Dshi_3981 at 40.802 kb on + strand, within Dshi_3982 at 40.802 kb on + strand, within Dshi_3982 at 40.822 kb on + strand, within Dshi_3982 at 40.843 kb on + strand, within Dshi_3982 at 40.883 kb on + strand, within Dshi_3982
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. perchlorate 50 mM remove 38,883 + Dshi_3979 0.63 +1.2 38,883 + Dshi_3979 0.63 -0.8 38,883 + Dshi_3979 0.63 -0.7 38,891 - Dshi_3979 0.64 +0.4 38,932 - Dshi_3979 0.69 -0.2 38,951 + Dshi_3979 0.72 -0.4 39,107 - +0.2 39,165 + +0.6 39,180 + +0.3 39,192 + -0.2 39,357 + Dshi_3980 0.24 -0.4 39,366 + Dshi_3980 0.26 +0.6 39,366 + Dshi_3980 0.26 -0.2 39,384 + Dshi_3980 0.29 +0.3 39,392 - Dshi_3980 0.30 +1.4 39,392 - Dshi_3980 0.30 -1.2 39,394 + Dshi_3980 0.30 +0.6 39,437 - Dshi_3980 0.37 -0.5 39,463 - Dshi_3980 0.41 +0.8 39,474 + Dshi_3980 0.42 +0.1 39,529 - Dshi_3980 0.51 -1.0 39,531 + Dshi_3980 0.51 -1.0 39,574 - Dshi_3980 0.58 -0.1 39,689 + Dshi_3980 0.75 +0.4 39,715 + Dshi_3980 0.79 +1.5 39,721 + Dshi_3980 0.80 -0.2 39,724 + Dshi_3980 0.81 +0.1 39,732 - Dshi_3980 0.82 -1.0 39,782 + Dshi_3980 0.89 +2.0 39,786 + +1.2 39,789 - -0.2 39,790 - +0.0 39,790 - -0.1 39,801 + +1.1 39,801 + +0.5 39,803 - -0.4 39,809 - -0.2 39,844 + +0.8 39,889 - -2.1 39,912 - +1.2 39,962 + Dshi_3981 0.16 -0.4 39,962 + Dshi_3981 0.16 -0.8 39,962 + Dshi_3981 0.16 +0.5 39,962 + Dshi_3981 0.16 -0.2 39,970 - Dshi_3981 0.17 +0.2 39,973 - Dshi_3981 0.17 -0.2 39,981 - Dshi_3981 0.18 -1.9 40,000 - Dshi_3981 0.21 -1.2 40,072 - Dshi_3981 0.31 -0.4 40,073 + Dshi_3981 0.32 -1.5 40,118 + Dshi_3981 0.38 -0.4 40,161 - Dshi_3981 0.44 +0.5 40,268 - Dshi_3981 0.60 +1.2 40,322 - Dshi_3981 0.67 -0.9 40,802 + Dshi_3982 0.19 -0.8 40,802 + Dshi_3982 0.19 -0.1 40,822 + Dshi_3982 0.20 -1.2 40,843 + Dshi_3982 0.22 +1.2 40,883 + Dshi_3982 0.24 -0.9
Or see this region's nucleotide sequence