Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4467 and tRNA-Trp are separated by 99 nucleotides tRNA-Trp and BT4468 are separated by 65 nucleotides BT4468 and BT4469 are separated by 54 nucleotides BT4469 and BT4470 are separated by 226 nucleotides
BT4467: BT4467 - hypothetical protein (NCBI ptt file), at 5,877,262 to 5,877,696
BT4467
BT_t68: tRNA-Trp - tRNA-Trp (RefSeq), at 5,877,796 to 5,877,871
tRNA-Trp
BT4468: BT4468 - conserved hypothetical protein (NCBI ptt file), at 5,877,937 to 5,878,290
BT4468
BT4469: BT4469 - conserved hypothetical protein (NCBI ptt file), at 5,878,345 to 5,878,827
BT4469
BT4470: BT4470 - outer membrane protein (NCBI ptt file), at 5,879,054 to 5,882,161
BT4470
Position (kb)
5878
5879 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5877.357 kb on + strand, within BT4467 at 5877.501 kb on + strand, within BT4467 at 5877.511 kb on + strand, within BT4467 at 5877.579 kb on + strand, within BT4467 at 5877.580 kb on + strand, within BT4467 at 5877.594 kb on + strand, within BT4467 at 5877.600 kb on + strand, within BT4467 at 5877.728 kb on + strand at 5877.751 kb on - strand at 5877.751 kb on - strand at 5877.754 kb on + strand at 5877.781 kb on + strand at 5877.782 kb on - strand at 5878.096 kb on - strand, within BT4468 at 5878.096 kb on - strand, within BT4468 at 5878.130 kb on - strand, within BT4468 at 5878.162 kb on + strand, within BT4468 at 5878.254 kb on + strand, within BT4468 at 5878.255 kb on - strand at 5878.294 kb on - strand at 5878.315 kb on - strand at 5878.325 kb on + strand at 5878.325 kb on + strand at 5878.332 kb on + strand at 5878.375 kb on - strand at 5878.415 kb on + strand, within BT4469 at 5878.585 kb on + strand, within BT4469 at 5878.586 kb on - strand, within BT4469 at 5878.657 kb on - strand, within BT4469 at 5878.795 kb on - strand at 5879.104 kb on - strand at 5879.204 kb on - strand at 5879.204 kb on - strand at 5879.208 kb on - strand at 5879.366 kb on - strand, within BT4470 at 5879.383 kb on - strand, within BT4470 at 5879.424 kb on + strand, within BT4470 at 5879.466 kb on - strand, within BT4470 at 5879.466 kb on - strand, within BT4470 at 5879.645 kb on + strand, within BT4470 at 5879.645 kb on + strand, within BT4470 at 5879.652 kb on + strand, within BT4470 at 5879.653 kb on - strand, within BT4470
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 5,877,357 + BT4467 0.22 -0.1 5,877,501 + BT4467 0.55 +0.1 5,877,511 + BT4467 0.57 -2.2 5,877,579 + BT4467 0.73 +0.0 5,877,580 + BT4467 0.73 +0.9 5,877,594 + BT4467 0.76 -0.2 5,877,600 + BT4467 0.78 -2.6 5,877,728 + +1.0 5,877,751 - -0.2 5,877,751 - -3.5 5,877,754 + -0.2 5,877,781 + -2.9 5,877,782 - +0.4 5,878,096 - BT4468 0.45 -2.6 5,878,096 - BT4468 0.45 +0.8 5,878,130 - BT4468 0.55 +0.2 5,878,162 + BT4468 0.64 -2.7 5,878,254 + BT4468 0.90 +1.5 5,878,255 - -2.2 5,878,294 - -0.5 5,878,315 - -0.3 5,878,325 + +2.1 5,878,325 + -0.8 5,878,332 + +2.5 5,878,375 - +0.0 5,878,415 + BT4469 0.14 -1.3 5,878,585 + BT4469 0.50 -2.4 5,878,586 - BT4469 0.50 -2.5 5,878,657 - BT4469 0.65 -2.2 5,878,795 - -2.0 5,879,104 - +2.5 5,879,204 - -1.8 5,879,204 - -0.5 5,879,208 - -1.1 5,879,366 - BT4470 0.10 -2.2 5,879,383 - BT4470 0.11 -2.2 5,879,424 + BT4470 0.12 -1.5 5,879,466 - BT4470 0.13 -0.2 5,879,466 - BT4470 0.13 +0.3 5,879,645 + BT4470 0.19 +0.5 5,879,645 + BT4470 0.19 -0.8 5,879,652 + BT4470 0.19 -0.1 5,879,653 - BT4470 0.19 -0.1
Or see this region's nucleotide sequence