Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3196 and BT3197 are separated by 423 nucleotides BT3197 and BT3198 overlap by 13 nucleotides
BT3196: BT3196 - hypothetical protein (NCBI ptt file), at 4,086,246 to 4,086,956
BT3196
BT3197: BT3197 - hypothetical protein (NCBI ptt file), at 4,087,380 to 4,087,700
BT3197
BT3198: BT3198 - conserved hypothetical protein (NCBI ptt file), at 4,087,688 to 4,088,596
BT3198
Position (kb)
4087
4088 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4086.386 kb on - strand, within BT3196 at 4086.434 kb on - strand, within BT3196 at 4086.449 kb on + strand, within BT3196 at 4086.468 kb on - strand, within BT3196 at 4086.533 kb on - strand, within BT3196 at 4086.580 kb on - strand, within BT3196 at 4086.585 kb on - strand, within BT3196 at 4086.585 kb on - strand, within BT3196 at 4086.587 kb on - strand, within BT3196 at 4086.641 kb on - strand, within BT3196 at 4086.641 kb on - strand, within BT3196 at 4086.641 kb on - strand, within BT3196 at 4086.730 kb on - strand, within BT3196 at 4087.252 kb on - strand at 4087.252 kb on - strand at 4087.252 kb on - strand at 4087.256 kb on - strand at 4087.274 kb on - strand at 4087.276 kb on - strand at 4087.285 kb on - strand at 4087.296 kb on - strand at 4087.307 kb on - strand at 4087.455 kb on - strand, within BT3197 at 4087.650 kb on - strand, within BT3197 at 4087.683 kb on - strand at 4087.683 kb on - strand at 4087.738 kb on + strand at 4087.766 kb on - strand at 4087.839 kb on - strand, within BT3198 at 4087.841 kb on + strand, within BT3198 at 4087.841 kb on + strand, within BT3198 at 4087.842 kb on - strand, within BT3198 at 4087.843 kb on + strand, within BT3198 at 4087.894 kb on + strand, within BT3198 at 4087.895 kb on - strand, within BT3198 at 4087.919 kb on - strand, within BT3198 at 4087.919 kb on - strand, within BT3198 at 4088.009 kb on + strand, within BT3198 at 4088.090 kb on + strand, within BT3198 at 4088.091 kb on - strand, within BT3198 at 4088.188 kb on + strand, within BT3198 at 4088.275 kb on - strand, within BT3198 at 4088.290 kb on - strand, within BT3198 at 4088.372 kb on - strand, within BT3198 at 4088.469 kb on - strand, within BT3198 at 4088.528 kb on - strand at 4088.528 kb on - strand at 4088.648 kb on - strand at 4088.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 4,086,386 - BT3196 0.20 +1.7 4,086,434 - BT3196 0.26 -0.6 4,086,449 + BT3196 0.29 +0.6 4,086,468 - BT3196 0.31 +0.6 4,086,533 - BT3196 0.40 +1.7 4,086,580 - BT3196 0.47 -0.2 4,086,585 - BT3196 0.48 -0.3 4,086,585 - BT3196 0.48 -1.0 4,086,587 - BT3196 0.48 -0.0 4,086,641 - BT3196 0.56 +1.1 4,086,641 - BT3196 0.56 -3.8 4,086,641 - BT3196 0.56 +0.6 4,086,730 - BT3196 0.68 +0.7 4,087,252 - +0.7 4,087,252 - -2.1 4,087,252 - +0.4 4,087,256 - +0.1 4,087,274 - +1.3 4,087,276 - -0.1 4,087,285 - -0.5 4,087,296 - +1.7 4,087,307 - -3.4 4,087,455 - BT3197 0.23 -3.3 4,087,650 - BT3197 0.84 -2.6 4,087,683 - -0.3 4,087,683 - -0.3 4,087,738 + -2.2 4,087,766 - -2.3 4,087,839 - BT3198 0.17 +0.1 4,087,841 + BT3198 0.17 -0.1 4,087,841 + BT3198 0.17 +1.7 4,087,842 - BT3198 0.17 +0.9 4,087,843 + BT3198 0.17 -3.2 4,087,894 + BT3198 0.23 -0.1 4,087,895 - BT3198 0.23 +1.7 4,087,919 - BT3198 0.25 -2.0 4,087,919 - BT3198 0.25 +1.4 4,088,009 + BT3198 0.35 -0.5 4,088,090 + BT3198 0.44 -0.6 4,088,091 - BT3198 0.44 +1.2 4,088,188 + BT3198 0.55 -1.3 4,088,275 - BT3198 0.65 +0.7 4,088,290 - BT3198 0.66 +0.6 4,088,372 - BT3198 0.75 -1.7 4,088,469 - BT3198 0.86 -1.0 4,088,528 - -1.0 4,088,528 - -1.6 4,088,648 - -1.2 4,088,665 - -2.5
Or see this region's nucleotide sequence