Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2451 and BT2452 are separated by 5 nucleotides BT2452 and BT2453 are separated by 8 nucleotides BT2453 and BT2454 are separated by 135 nucleotides BT2454 and BT2455 are separated by 99 nucleotides
BT2451: BT2451 - putative pyrogenic exotoxin B (NCBI ptt file), at 3,068,967 to 3,070,247
BT2451
BT2452: BT2452 - hypothetical protein (NCBI ptt file), at 3,070,253 to 3,070,648
BT2452
BT2453: BT2453 - hypothetical protein (NCBI ptt file), at 3,070,657 to 3,070,863
BT2453
BT2454: BT2454 - hypothetical protein (NCBI ptt file), at 3,070,999 to 3,071,256
BT2454
BT2455: BT2455 - reverse transcriptase/maturase family protein (NCBI ptt file), at 3,071,356 to 3,072,045
BT2455
Position (kb)
3070
3071 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3069.697 kb on - strand, within BT2451 at 3069.697 kb on - strand, within BT2451 at 3069.698 kb on + strand, within BT2451 at 3069.699 kb on - strand, within BT2451 at 3069.699 kb on - strand, within BT2451 at 3069.699 kb on - strand, within BT2451 at 3069.848 kb on + strand, within BT2451 at 3069.892 kb on + strand, within BT2451 at 3069.892 kb on + strand, within BT2451 at 3069.900 kb on - strand, within BT2451 at 3069.903 kb on - strand, within BT2451 at 3069.925 kb on + strand, within BT2451 at 3069.926 kb on - strand, within BT2451 at 3069.962 kb on + strand, within BT2451 at 3069.962 kb on + strand, within BT2451 at 3070.034 kb on + strand, within BT2451 at 3070.084 kb on - strand, within BT2451 at 3070.094 kb on - strand, within BT2451 at 3070.118 kb on + strand, within BT2451 at 3070.118 kb on + strand, within BT2451 at 3070.125 kb on + strand at 3070.129 kb on - strand at 3070.163 kb on + strand at 3070.196 kb on + strand at 3070.207 kb on - strand at 3070.229 kb on + strand at 3070.229 kb on + strand at 3070.230 kb on - strand at 3070.237 kb on + strand at 3070.245 kb on + strand at 3070.269 kb on - strand at 3070.293 kb on + strand, within BT2452 at 3070.342 kb on + strand, within BT2452 at 3070.342 kb on + strand, within BT2452 at 3070.347 kb on + strand, within BT2452 at 3070.348 kb on - strand, within BT2452 at 3070.402 kb on - strand, within BT2452 at 3070.447 kb on + strand, within BT2452 at 3070.459 kb on - strand, within BT2452 at 3070.460 kb on + strand, within BT2452 at 3070.461 kb on - strand, within BT2452 at 3070.462 kb on + strand, within BT2452 at 3070.462 kb on + strand, within BT2452 at 3070.463 kb on - strand, within BT2452 at 3070.463 kb on - strand, within BT2452 at 3070.508 kb on + strand, within BT2452 at 3070.508 kb on + strand, within BT2452 at 3070.582 kb on + strand, within BT2452 at 3070.582 kb on + strand, within BT2452 at 3070.592 kb on - strand, within BT2452 at 3070.592 kb on - strand, within BT2452 at 3070.592 kb on - strand, within BT2452 at 3070.594 kb on - strand, within BT2452 at 3070.611 kb on - strand at 3070.612 kb on + strand at 3070.613 kb on - strand at 3070.613 kb on - strand at 3070.616 kb on - strand at 3070.621 kb on + strand at 3070.622 kb on - strand at 3070.622 kb on - strand at 3070.624 kb on + strand at 3070.624 kb on + strand at 3070.627 kb on - strand at 3070.630 kb on - strand at 3070.698 kb on + strand, within BT2453 at 3070.698 kb on + strand, within BT2453 at 3070.705 kb on - strand, within BT2453 at 3070.736 kb on + strand, within BT2453 at 3070.736 kb on + strand, within BT2453 at 3070.773 kb on + strand, within BT2453 at 3070.776 kb on - strand, within BT2453 at 3070.860 kb on - strand at 3070.883 kb on + strand at 3070.884 kb on - strand at 3070.914 kb on + strand at 3070.914 kb on + strand at 3070.914 kb on + strand at 3070.914 kb on + strand at 3070.915 kb on - strand at 3070.915 kb on - strand at 3070.915 kb on - strand at 3070.915 kb on - strand at 3070.915 kb on - strand at 3070.919 kb on - strand at 3070.919 kb on - strand at 3070.925 kb on - strand at 3070.993 kb on + strand at 3070.993 kb on + strand at 3070.993 kb on + strand at 3070.999 kb on - strand at 3071.009 kb on + strand at 3071.010 kb on - strand at 3071.016 kb on + strand at 3071.017 kb on - strand at 3071.024 kb on + strand at 3071.024 kb on + strand at 3071.025 kb on - strand, within BT2454 at 3071.072 kb on + strand, within BT2454 at 3071.073 kb on - strand, within BT2454 at 3071.125 kb on - strand, within BT2454 at 3071.138 kb on - strand, within BT2454 at 3071.141 kb on + strand, within BT2454 at 3071.141 kb on + strand, within BT2454 at 3071.156 kb on + strand, within BT2454 at 3071.193 kb on + strand, within BT2454 at 3071.194 kb on - strand, within BT2454 at 3071.194 kb on - strand, within BT2454 at 3071.228 kb on - strand, within BT2454 at 3071.229 kb on + strand, within BT2454 at 3071.230 kb on - strand, within BT2454 at 3071.230 kb on - strand, within BT2454 at 3071.244 kb on - strand at 3071.255 kb on - strand at 3071.319 kb on + strand at 3071.343 kb on + strand at 3071.364 kb on + strand at 3071.364 kb on + strand at 3071.364 kb on + strand at 3071.434 kb on - strand, within BT2455 at 3071.434 kb on - strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.456 kb on + strand, within BT2455 at 3071.513 kb on - strand, within BT2455 at 3071.539 kb on + strand, within BT2455 at 3071.540 kb on - strand, within BT2455 at 3071.548 kb on - strand, within BT2455 at 3071.548 kb on - strand, within BT2455 at 3071.571 kb on + strand, within BT2455 at 3071.574 kb on + strand, within BT2455 at 3071.574 kb on + strand, within BT2455 at 3071.574 kb on + strand, within BT2455 at 3071.583 kb on + strand, within BT2455 at 3071.592 kb on + strand, within BT2455 at 3071.599 kb on - strand, within BT2455 at 3071.599 kb on - strand, within BT2455 at 3071.678 kb on - strand, within BT2455 at 3071.690 kb on - strand, within BT2455 at 3071.690 kb on - strand, within BT2455 at 3071.722 kb on + strand, within BT2455 at 3071.722 kb on + strand, within BT2455 at 3071.746 kb on + strand, within BT2455 at 3071.838 kb on + strand, within BT2455 at 3071.839 kb on - strand, within BT2455 at 3071.858 kb on + strand, within BT2455 at 3071.858 kb on + strand, within BT2455
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 3,069,697 - BT2451 0.57 +1.0 3,069,697 - BT2451 0.57 -0.0 3,069,698 + BT2451 0.57 -0.0 3,069,699 - BT2451 0.57 -2.5 3,069,699 - BT2451 0.57 -2.7 3,069,699 - BT2451 0.57 -1.8 3,069,848 + BT2451 0.69 +0.6 3,069,892 + BT2451 0.72 -1.0 3,069,892 + BT2451 0.72 -0.3 3,069,900 - BT2451 0.73 +1.8 3,069,903 - BT2451 0.73 -0.4 3,069,925 + BT2451 0.75 -1.4 3,069,926 - BT2451 0.75 -3.0 3,069,962 + BT2451 0.78 +0.2 3,069,962 + BT2451 0.78 -0.2 3,070,034 + BT2451 0.83 +1.0 3,070,084 - BT2451 0.87 -3.5 3,070,094 - BT2451 0.88 -0.2 3,070,118 + BT2451 0.90 -0.8 3,070,118 + BT2451 0.90 -0.3 3,070,125 + -0.0 3,070,129 - -0.2 3,070,163 + -1.9 3,070,196 + +2.8 3,070,207 - -3.1 3,070,229 + +2.0 3,070,229 + -1.0 3,070,230 - -0.1 3,070,237 + -0.3 3,070,245 + +1.6 3,070,269 - +0.3 3,070,293 + BT2452 0.10 -0.8 3,070,342 + BT2452 0.22 -1.0 3,070,342 + BT2452 0.22 +1.2 3,070,347 + BT2452 0.24 -0.8 3,070,348 - BT2452 0.24 -0.5 3,070,402 - BT2452 0.38 +1.6 3,070,447 + BT2452 0.49 -1.4 3,070,459 - BT2452 0.52 -0.4 3,070,460 + BT2452 0.52 +0.2 3,070,461 - BT2452 0.53 +0.1 3,070,462 + BT2452 0.53 +1.3 3,070,462 + BT2452 0.53 -0.5 3,070,463 - BT2452 0.53 +0.5 3,070,463 - BT2452 0.53 -1.9 3,070,508 + BT2452 0.64 +0.9 3,070,508 + BT2452 0.64 -3.0 3,070,582 + BT2452 0.83 -2.0 3,070,582 + BT2452 0.83 -0.2 3,070,592 - BT2452 0.86 -3.1 3,070,592 - BT2452 0.86 +0.9 3,070,592 - BT2452 0.86 -0.7 3,070,594 - BT2452 0.86 +2.4 3,070,611 - -4.3 3,070,612 + -1.0 3,070,613 - -0.8 3,070,613 - -1.7 3,070,616 - -1.6 3,070,621 + -0.2 3,070,622 - +1.3 3,070,622 - -2.9 3,070,624 + -3.8 3,070,624 + -0.5 3,070,627 - +1.0 3,070,630 - -1.8 3,070,698 + BT2453 0.20 -0.1 3,070,698 + BT2453 0.20 -3.8 3,070,705 - BT2453 0.23 -2.8 3,070,736 + BT2453 0.38 -0.2 3,070,736 + BT2453 0.38 +0.3 3,070,773 + BT2453 0.56 -3.1 3,070,776 - BT2453 0.57 -2.8 3,070,860 - -0.6 3,070,883 + +0.5 3,070,884 - +0.1 3,070,914 + +0.2 3,070,914 + -2.3 3,070,914 + +1.6 3,070,914 + +0.2 3,070,915 - -0.4 3,070,915 - -0.1 3,070,915 - +0.2 3,070,915 - -1.5 3,070,915 - +0.4 3,070,919 - +0.6 3,070,919 - -2.7 3,070,925 - -1.8 3,070,993 + +2.2 3,070,993 + -2.4 3,070,993 + -0.8 3,070,999 - +0.5 3,071,009 + +0.9 3,071,010 - +0.6 3,071,016 + +2.2 3,071,017 - -0.1 3,071,024 + +3.2 3,071,024 + +1.0 3,071,025 - BT2454 0.10 -0.4 3,071,072 + BT2454 0.28 -3.6 3,071,073 - BT2454 0.29 -1.8 3,071,125 - BT2454 0.49 +1.3 3,071,138 - BT2454 0.54 +0.9 3,071,141 + BT2454 0.55 -0.8 3,071,141 + BT2454 0.55 -0.6 3,071,156 + BT2454 0.61 +0.6 3,071,193 + BT2454 0.75 -3.2 3,071,194 - BT2454 0.76 -2.8 3,071,194 - BT2454 0.76 +0.8 3,071,228 - BT2454 0.89 +0.8 3,071,229 + BT2454 0.89 +1.6 3,071,230 - BT2454 0.90 +0.5 3,071,230 - BT2454 0.90 -1.9 3,071,244 - -0.5 3,071,255 - -1.0 3,071,319 + +0.6 3,071,343 + -1.0 3,071,364 + +1.2 3,071,364 + +0.2 3,071,364 + +0.2 3,071,434 - BT2455 0.11 -0.9 3,071,434 - BT2455 0.11 +0.6 3,071,439 + BT2455 0.12 -0.4 3,071,439 + BT2455 0.12 -0.1 3,071,439 + BT2455 0.12 -2.7 3,071,439 + BT2455 0.12 -2.9 3,071,456 + BT2455 0.14 +1.6 3,071,513 - BT2455 0.23 +1.1 3,071,539 + BT2455 0.27 -1.0 3,071,540 - BT2455 0.27 +0.6 3,071,548 - BT2455 0.28 +0.3 3,071,548 - BT2455 0.28 +1.6 3,071,571 + BT2455 0.31 -0.5 3,071,574 + BT2455 0.32 -0.8 3,071,574 + BT2455 0.32 +0.2 3,071,574 + BT2455 0.32 +1.0 3,071,583 + BT2455 0.33 +1.0 3,071,592 + BT2455 0.34 -4.0 3,071,599 - BT2455 0.35 -2.2 3,071,599 - BT2455 0.35 +1.6 3,071,678 - BT2455 0.47 +1.2 3,071,690 - BT2455 0.48 -3.0 3,071,690 - BT2455 0.48 +0.6 3,071,722 + BT2455 0.53 -1.5 3,071,722 + BT2455 0.53 -2.2 3,071,746 + BT2455 0.57 +0.3 3,071,838 + BT2455 0.70 +0.2 3,071,839 - BT2455 0.70 -0.7 3,071,858 + BT2455 0.73 +0.8 3,071,858 + BT2455 0.73 -2.3
Or see this region's nucleotide sequence