Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2279 and BT2280 are separated by 1 nucleotides BT2280 and BT2281 are separated by 186 nucleotides BT2281 and BT2282 are separated by 757 nucleotides
BT2279: BT2279 - putative ribose phosphate pyrophosphokinase (NCBI ptt file), at 2,859,673 to 2,860,593
BT2279
BT2280: BT2280 - hypothetical protein (NCBI ptt file), at 2,860,595 to 2,860,969
BT2280
BT2281: BT2281 - hypothetical protein (NCBI ptt file), at 2,861,156 to 2,861,428
BT2281
BT2282: BT2282 - conserved hypothetical protein (NCBI ptt file), at 2,862,186 to 2,862,758
BT2282
Position (kb)
2861
2862 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2860.156 kb on - strand, within BT2279 at 2860.161 kb on + strand, within BT2279 at 2860.466 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.668 kb on + strand, within BT2280 at 2860.668 kb on + strand, within BT2280 at 2860.669 kb on - strand, within BT2280 at 2860.810 kb on - strand, within BT2280 at 2860.856 kb on - strand, within BT2280 at 2860.882 kb on - strand, within BT2280 at 2860.912 kb on + strand, within BT2280 at 2860.979 kb on + strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.982 kb on + strand at 2860.982 kb on + strand at 2861.036 kb on + strand at 2861.059 kb on + strand at 2861.093 kb on + strand at 2861.093 kb on + strand at 2861.094 kb on - strand at 2861.094 kb on - strand at 2861.142 kb on - strand at 2861.142 kb on - strand at 2861.182 kb on + strand at 2861.182 kb on + strand at 2861.186 kb on + strand, within BT2281 at 2861.197 kb on - strand, within BT2281 at 2861.304 kb on - strand, within BT2281 at 2861.342 kb on - strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.428 kb on + strand at 2861.440 kb on + strand at 2861.441 kb on - strand at 2861.444 kb on - strand at 2861.448 kb on + strand at 2861.521 kb on + strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.561 kb on + strand at 2861.617 kb on - strand at 2861.617 kb on - strand at 2861.668 kb on - strand at 2861.668 kb on - strand at 2861.671 kb on + strand at 2861.683 kb on - strand at 2861.715 kb on + strand at 2861.952 kb on - strand at 2862.136 kb on - strand at 2862.257 kb on - strand, within BT2282 at 2862.270 kb on - strand, within BT2282 at 2862.346 kb on - strand, within BT2282 at 2862.385 kb on - strand, within BT2282 at 2862.385 kb on - strand, within BT2282
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 2,860,156 - BT2279 0.52 +1.5 2,860,161 + BT2279 0.53 +1.5 2,860,466 + BT2279 0.86 -1.5 2,860,479 + BT2279 0.88 +1.5 2,860,479 + BT2279 0.88 +0.4 2,860,668 + BT2280 0.19 -2.5 2,860,668 + BT2280 0.19 -0.5 2,860,669 - BT2280 0.20 +3.1 2,860,810 - BT2280 0.57 +1.5 2,860,856 - BT2280 0.70 -2.0 2,860,882 - BT2280 0.77 +1.5 2,860,912 + BT2280 0.85 -0.0 2,860,979 + +0.2 2,860,980 - -2.3 2,860,980 - -1.8 2,860,980 - -2.7 2,860,982 + +0.5 2,860,982 + +0.4 2,861,036 + -0.4 2,861,059 + -1.6 2,861,093 + +3.9 2,861,093 + +1.0 2,861,094 - -0.8 2,861,094 - +0.3 2,861,142 - +0.8 2,861,142 - +0.5 2,861,182 + +0.2 2,861,182 + -0.5 2,861,186 + BT2281 0.11 -2.7 2,861,197 - BT2281 0.15 -1.3 2,861,304 - BT2281 0.54 -1.8 2,861,342 - BT2281 0.68 -1.6 2,861,367 + BT2281 0.77 -2.4 2,861,367 + BT2281 0.77 -1.5 2,861,428 + -0.7 2,861,440 + +1.7 2,861,441 - -0.8 2,861,444 - +1.9 2,861,448 + -0.9 2,861,521 + -0.8 2,861,536 - +0.2 2,861,536 - -2.3 2,861,536 - -0.8 2,861,536 - +0.8 2,861,536 - +1.5 2,861,561 + -2.5 2,861,617 - -0.1 2,861,617 - -2.6 2,861,668 - -3.2 2,861,668 - -0.7 2,861,671 + -0.5 2,861,683 - -2.2 2,861,715 + +0.7 2,861,952 - +1.1 2,862,136 - +2.3 2,862,257 - BT2282 0.12 -2.9 2,862,270 - BT2282 0.15 -0.5 2,862,346 - BT2282 0.28 -1.3 2,862,385 - BT2282 0.35 -1.5 2,862,385 - BT2282 0.35 +1.5
Or see this region's nucleotide sequence