Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2207 and BT2208 overlap by 4 nucleotides BT2208 and BT2209 are separated by 75 nucleotides BT2209 and BT2210 overlap by 17 nucleotides BT2210 and BT2211 overlap by 6 nucleotides BT2211 and BT2212 are separated by 59 nucleotides
BT2207: BT2207 - putative ATPase/GTPase (NCBI ptt file), at 2,777,429 to 2,777,842
BT2207
BT2208: BT2208 - hypothetical protein (NCBI ptt file), at 2,777,839 to 2,778,063
BT2208
BT2209: BT2209 - putative nucleotidyltransferase (NCBI ptt file), at 2,778,139 to 2,778,432
BT2209
BT2210: BT2210 - conserved hypothetical protein (NCBI ptt file), at 2,778,416 to 2,778,793
BT2210
BT2211: BT2211 - conserved hypothetical protein (NCBI ptt file), at 2,778,788 to 2,780,098
BT2211
BT2212: BT2212 - magnesium chelatase, subunit I, putative ATPase (NCBI ptt file), at 2,780,158 to 2,781,132
BT2212
Position (kb)
2778
2779
2780
2781 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2777.838 kb on + strand at 2777.858 kb on + strand at 2778.009 kb on + strand, within BT2208 at 2778.010 kb on - strand, within BT2208 at 2778.026 kb on + strand, within BT2208 at 2778.039 kb on - strand, within BT2208 at 2778.039 kb on - strand, within BT2208 at 2778.065 kb on - strand at 2778.065 kb on - strand at 2778.134 kb on + strand at 2778.223 kb on - strand, within BT2209 at 2778.340 kb on - strand, within BT2209 at 2778.424 kb on + strand at 2778.425 kb on - strand at 2778.448 kb on + strand at 2778.479 kb on - strand, within BT2210 at 2778.502 kb on + strand, within BT2210 at 2778.502 kb on + strand, within BT2210 at 2778.505 kb on - strand, within BT2210 at 2778.595 kb on + strand, within BT2210 at 2778.596 kb on - strand, within BT2210 at 2778.720 kb on + strand, within BT2210 at 2778.726 kb on + strand, within BT2210 at 2778.775 kb on + strand at 2778.786 kb on + strand at 2778.821 kb on + strand at 2778.823 kb on + strand at 2778.823 kb on + strand at 2778.839 kb on - strand at 2778.892 kb on + strand at 2778.949 kb on - strand, within BT2211 at 2779.096 kb on - strand, within BT2211 at 2779.096 kb on - strand, within BT2211 at 2779.174 kb on + strand, within BT2211 at 2779.188 kb on + strand, within BT2211 at 2779.213 kb on + strand, within BT2211 at 2779.214 kb on - strand, within BT2211 at 2779.328 kb on - strand, within BT2211 at 2779.389 kb on - strand, within BT2211 at 2779.433 kb on + strand, within BT2211 at 2779.433 kb on + strand, within BT2211 at 2779.496 kb on + strand, within BT2211 at 2779.501 kb on - strand, within BT2211 at 2779.547 kb on - strand, within BT2211 at 2779.573 kb on + strand, within BT2211 at 2779.574 kb on - strand, within BT2211 at 2779.623 kb on + strand, within BT2211 at 2779.746 kb on + strand, within BT2211 at 2779.746 kb on + strand, within BT2211 at 2779.813 kb on + strand, within BT2211 at 2779.821 kb on - strand, within BT2211 at 2779.957 kb on + strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.959 kb on + strand, within BT2211 at 2779.960 kb on - strand, within BT2211 at 2779.960 kb on - strand, within BT2211 at 2780.048 kb on + strand at 2780.076 kb on + strand at 2780.077 kb on - strand at 2780.379 kb on - strand, within BT2212 at 2780.380 kb on + strand, within BT2212 at 2780.424 kb on - strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.443 kb on + strand, within BT2212 at 2780.524 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.582 kb on + strand, within BT2212 at 2780.676 kb on + strand, within BT2212 at 2780.681 kb on + strand, within BT2212 at 2780.682 kb on - strand, within BT2212 at 2780.698 kb on - strand, within BT2212 at 2780.719 kb on + strand, within BT2212 at 2780.720 kb on - strand, within BT2212 at 2780.795 kb on - strand, within BT2212 at 2780.848 kb on - strand, within BT2212 at 2780.987 kb on - strand, within BT2212
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 2,777,838 + -1.3 2,777,858 + -1.9 2,778,009 + BT2208 0.76 +0.5 2,778,010 - BT2208 0.76 -0.5 2,778,026 + BT2208 0.83 -2.1 2,778,039 - BT2208 0.89 -2.1 2,778,039 - BT2208 0.89 -2.2 2,778,065 - -0.6 2,778,065 - +0.1 2,778,134 + +0.7 2,778,223 - BT2209 0.29 -3.0 2,778,340 - BT2209 0.68 -1.8 2,778,424 + -0.7 2,778,425 - -0.8 2,778,448 + +1.1 2,778,479 - BT2210 0.17 +0.7 2,778,502 + BT2210 0.23 -1.1 2,778,502 + BT2210 0.23 -2.3 2,778,505 - BT2210 0.24 -1.1 2,778,595 + BT2210 0.47 -1.3 2,778,596 - BT2210 0.48 +0.7 2,778,720 + BT2210 0.80 -0.6 2,778,726 + BT2210 0.82 -0.1 2,778,775 + -0.2 2,778,786 + -0.3 2,778,821 + +0.9 2,778,823 + -2.5 2,778,823 + -0.3 2,778,839 - +0.0 2,778,892 + -0.1 2,778,949 - BT2211 0.12 -3.4 2,779,096 - BT2211 0.23 -1.1 2,779,096 - BT2211 0.23 +0.6 2,779,174 + BT2211 0.29 -0.5 2,779,188 + BT2211 0.31 +1.1 2,779,213 + BT2211 0.32 -2.1 2,779,214 - BT2211 0.32 -3.2 2,779,328 - BT2211 0.41 +1.5 2,779,389 - BT2211 0.46 +0.5 2,779,433 + BT2211 0.49 +0.7 2,779,433 + BT2211 0.49 -0.3 2,779,496 + BT2211 0.54 -2.2 2,779,501 - BT2211 0.54 -1.5 2,779,547 - BT2211 0.58 +0.1 2,779,573 + BT2211 0.60 -0.5 2,779,574 - BT2211 0.60 -0.2 2,779,623 + BT2211 0.64 -0.2 2,779,746 + BT2211 0.73 -2.2 2,779,746 + BT2211 0.73 -0.1 2,779,813 + BT2211 0.78 -2.6 2,779,821 - BT2211 0.79 -0.8 2,779,957 + BT2211 0.89 -1.1 2,779,958 - BT2211 0.89 -3.7 2,779,958 - BT2211 0.89 -0.6 2,779,958 - BT2211 0.89 -0.2 2,779,959 + BT2211 0.89 -1.6 2,779,960 - BT2211 0.89 -0.5 2,779,960 - BT2211 0.89 -1.6 2,780,048 + -0.1 2,780,076 + +0.5 2,780,077 - +0.5 2,780,379 - BT2212 0.23 -0.1 2,780,380 + BT2212 0.23 +0.5 2,780,424 - BT2212 0.27 -3.0 2,780,436 + BT2212 0.29 -1.4 2,780,436 + BT2212 0.29 +2.4 2,780,443 + BT2212 0.29 -2.1 2,780,524 + BT2212 0.38 -0.1 2,780,538 + BT2212 0.39 -0.1 2,780,538 + BT2212 0.39 -1.3 2,780,582 + BT2212 0.43 +1.5 2,780,676 + BT2212 0.53 +1.5 2,780,681 + BT2212 0.54 +0.7 2,780,682 - BT2212 0.54 -2.7 2,780,698 - BT2212 0.55 +0.9 2,780,719 + BT2212 0.58 +0.4 2,780,720 - BT2212 0.58 -0.3 2,780,795 - BT2212 0.65 -2.2 2,780,848 - BT2212 0.71 -1.2 2,780,987 - BT2212 0.85 -0.5
Or see this region's nucleotide sequence