Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2012 and BT2013 are separated by 152 nucleotides BT2013 and BT2014 are separated by 113 nucleotides BT2014 and BT2015 are separated by 4 nucleotides
BT2012: BT2012 - conserved hypothetical protein, putative ATPase (NCBI ptt file), at 2,526,658 to 2,527,947
BT2012
BT2013: BT2013 - putative phosphohydrolases (NCBI ptt file), at 2,528,100 to 2,529,155
BT2013
BT2014: BT2014 - 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI ptt file), at 2,529,269 to 2,529,832
BT2014
BT2015: BT2015 - putative menaquinone biosynthesis protein (NCBI ptt file), at 2,529,837 to 2,530,166
BT2015
Position (kb)
2528
2529
2530 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2527.109 kb on + strand at 2527.109 kb on + strand, within BT2012 at 2527.114 kb on - strand, within BT2012 at 2527.192 kb on - strand, within BT2012 at 2527.296 kb on - strand, within BT2012 at 2527.296 kb on - strand, within BT2012 at 2527.350 kb on + strand, within BT2012 at 2527.388 kb on + strand, within BT2012 at 2527.388 kb on + strand, within BT2012 at 2527.463 kb on - strand, within BT2012 at 2527.522 kb on + strand, within BT2012 at 2527.566 kb on + strand, within BT2012 at 2527.567 kb on - strand, within BT2012 at 2527.634 kb on + strand, within BT2012 at 2527.687 kb on + strand, within BT2012 at 2527.699 kb on - strand, within BT2012 at 2527.702 kb on + strand, within BT2012 at 2527.703 kb on - strand, within BT2012 at 2527.706 kb on - strand, within BT2012 at 2527.806 kb on - strand, within BT2012 at 2527.922 kb on - strand at 2527.951 kb on + strand at 2527.973 kb on + strand at 2528.286 kb on + strand, within BT2013 at 2528.286 kb on + strand, within BT2013 at 2528.303 kb on + strand, within BT2013 at 2528.380 kb on + strand, within BT2013 at 2528.484 kb on + strand, within BT2013 at 2528.485 kb on - strand, within BT2013 at 2528.489 kb on + strand, within BT2013 at 2528.563 kb on + strand, within BT2013 at 2528.564 kb on - strand, within BT2013 at 2528.571 kb on - strand, within BT2013 at 2528.638 kb on - strand, within BT2013 at 2528.638 kb on - strand, within BT2013 at 2528.638 kb on - strand, within BT2013 at 2528.641 kb on + strand, within BT2013 at 2528.750 kb on - strand, within BT2013 at 2528.838 kb on + strand, within BT2013 at 2528.847 kb on - strand, within BT2013 at 2528.848 kb on + strand, within BT2013 at 2528.848 kb on + strand, within BT2013 at 2528.906 kb on + strand, within BT2013 at 2529.106 kb on - strand at 2529.106 kb on - strand at 2529.106 kb on - strand at 2529.139 kb on + strand at 2529.181 kb on + strand at 2529.182 kb on - strand at 2529.182 kb on - strand at 2529.182 kb on - strand at 2529.184 kb on - strand at 2529.184 kb on - strand at 2529.256 kb on + strand at 2529.256 kb on + strand at 2529.258 kb on + strand at 2529.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 2,527,109 + -0.1 2,527,109 + BT2012 0.35 +0.9 2,527,114 - BT2012 0.35 +1.5 2,527,192 - BT2012 0.41 +2.1 2,527,296 - BT2012 0.49 -3.4 2,527,296 - BT2012 0.49 -2.9 2,527,350 + BT2012 0.54 -1.7 2,527,388 + BT2012 0.57 +1.4 2,527,388 + BT2012 0.57 +1.5 2,527,463 - BT2012 0.62 -0.9 2,527,522 + BT2012 0.67 +0.2 2,527,566 + BT2012 0.70 -0.2 2,527,567 - BT2012 0.70 +0.5 2,527,634 + BT2012 0.76 -1.3 2,527,687 + BT2012 0.80 +0.1 2,527,699 - BT2012 0.81 +2.1 2,527,702 + BT2012 0.81 +0.4 2,527,703 - BT2012 0.81 -3.5 2,527,706 - BT2012 0.81 -0.9 2,527,806 - BT2012 0.89 -2.4 2,527,922 - +0.4 2,527,951 + -0.4 2,527,973 + -0.3 2,528,286 + BT2013 0.18 -1.1 2,528,286 + BT2013 0.18 -2.0 2,528,303 + BT2013 0.19 -0.1 2,528,380 + BT2013 0.27 +2.2 2,528,484 + BT2013 0.36 +1.1 2,528,485 - BT2013 0.36 +0.1 2,528,489 + BT2013 0.37 -1.0 2,528,563 + BT2013 0.44 +0.9 2,528,564 - BT2013 0.44 -3.0 2,528,571 - BT2013 0.45 -0.4 2,528,638 - BT2013 0.51 -0.4 2,528,638 - BT2013 0.51 -2.3 2,528,638 - BT2013 0.51 -2.0 2,528,641 + BT2013 0.51 +0.1 2,528,750 - BT2013 0.62 +2.5 2,528,838 + BT2013 0.70 -3.0 2,528,847 - BT2013 0.71 -0.5 2,528,848 + BT2013 0.71 -3.7 2,528,848 + BT2013 0.71 -3.6 2,528,906 + BT2013 0.76 -1.5 2,529,106 - -2.1 2,529,106 - -1.1 2,529,106 - +0.0 2,529,139 + -0.9 2,529,181 + +0.9 2,529,182 - -1.6 2,529,182 - +0.4 2,529,182 - -0.3 2,529,184 - +0.5 2,529,184 - -0.5 2,529,256 + -1.1 2,529,256 + -0.1 2,529,258 + +1.2 2,529,263 + -0.5
Or see this region's nucleotide sequence