Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1231 and tRNA-Ser are separated by 95 nucleotides tRNA-Ser and tRNA-Glu are separated by 63 nucleotides tRNA-Glu and BT1232 are separated by 67 nucleotides BT1232 and BT1233 are separated by 169 nucleotides
BT1231: BT1231 - hypothetical protein (NCBI ptt file), at 1,533,844 to 1,534,047
BT1231
BT_t21: tRNA-Ser - tRNA-Ser (RefSeq), at 1,534,143 to 1,534,232
tRNA-Ser
BT_t22: tRNA-Glu - tRNA-Glu (RefSeq), at 1,534,296 to 1,534,367
tRNA-Glu
BT1232: BT1232 - hypothetical protein (NCBI ptt file), at 1,534,435 to 1,534,641
BT1232
BT1233: BT1233 - hypothetical protein (NCBI ptt file), at 1,534,811 to 1,535,545
BT1233
Position (kb)
1534
1535 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1533.438 kb on + strand at 1533.438 kb on + strand at 1533.438 kb on + strand at 1533.478 kb on + strand at 1533.493 kb on + strand at 1533.545 kb on + strand at 1533.563 kb on + strand at 1533.597 kb on + strand at 1533.862 kb on - strand at 1533.912 kb on + strand, within BT1231 at 1534.036 kb on + strand at 1534.339 kb on + strand, within tRNA-Glu at 1534.340 kb on - strand, within tRNA-Glu at 1534.373 kb on - strand at 1534.377 kb on + strand at 1534.494 kb on + strand, within BT1232 at 1534.593 kb on + strand, within BT1232 at 1534.619 kb on - strand, within BT1232 at 1534.745 kb on + strand at 1534.809 kb on - strand at 1534.812 kb on + strand at 1534.921 kb on - strand, within BT1233 at 1534.923 kb on - strand, within BT1233 at 1535.177 kb on - strand, within BT1233 at 1535.182 kb on + strand, within BT1233 at 1535.206 kb on - strand, within BT1233 at 1535.219 kb on - strand, within BT1233 at 1535.248 kb on + strand, within BT1233 at 1535.352 kb on - strand, within BT1233 at 1535.399 kb on + strand, within BT1233 at 1535.399 kb on + strand, within BT1233 at 1535.400 kb on - strand, within BT1233 at 1535.466 kb on + strand, within BT1233 at 1535.467 kb on - strand, within BT1233 at 1535.468 kb on + strand, within BT1233 at 1535.545 kb on + strand at 1535.546 kb on - strand at 1535.547 kb on + strand at 1535.573 kb on - strand at 1535.573 kb on - strand at 1535.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 1,533,438 + -3.6 1,533,438 + +0.9 1,533,438 + -2.2 1,533,478 + +0.7 1,533,493 + +1.7 1,533,545 + -4.1 1,533,563 + -2.3 1,533,597 + -1.8 1,533,862 - -1.7 1,533,912 + BT1231 0.33 +1.3 1,534,036 + +0.3 1,534,339 + tRNA-Glu BT_t22 0.60 -2.5 1,534,340 - tRNA-Glu BT_t22 0.61 -1.3 1,534,373 - +0.2 1,534,377 + -2.5 1,534,494 + BT1232 0.29 -1.7 1,534,593 + BT1232 0.76 -2.7 1,534,619 - BT1232 0.89 -1.8 1,534,745 + -1.3 1,534,809 - +0.4 1,534,812 + +1.5 1,534,921 - BT1233 0.15 -0.5 1,534,923 - BT1233 0.15 -2.1 1,535,177 - BT1233 0.50 +0.1 1,535,182 + BT1233 0.50 -2.3 1,535,206 - BT1233 0.54 +1.3 1,535,219 - BT1233 0.56 +0.9 1,535,248 + BT1233 0.59 -3.1 1,535,352 - BT1233 0.74 -2.6 1,535,399 + BT1233 0.80 +1.8 1,535,399 + BT1233 0.80 +2.1 1,535,400 - BT1233 0.80 -1.4 1,535,466 + BT1233 0.89 +1.5 1,535,467 - BT1233 0.89 -0.8 1,535,468 + BT1233 0.89 -1.6 1,535,545 + -0.7 1,535,546 - -2.5 1,535,547 + -1.4 1,535,573 - -2.2 1,535,573 - -0.8 1,535,614 - -0.6
Or see this region's nucleotide sequence