Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0540 and BT0541 are separated by 5 nucleotides BT0541 and BT0542 are separated by 172 nucleotides BT0542 and BT0543 are separated by 121 nucleotides
BT0540: BT0540 - putative exopolyphosphatase (NCBI ptt file), at 664,074 to 664,991
BT0540
BT0541: BT0541 - Polyphosphate kinase (NCBI ptt file), at 664,997 to 667,114
BT0541
BT0542: BT0542 - conserved hypothetical protein (NCBI ptt file), at 667,287 to 667,865
BT0542
BT0543: BT0543 - glutamine synthetase (NCBI ptt file), at 667,987 to 670,176
BT0543
Position (kb) 664
665
666
667
668 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 664.100 kb on + strand at 664.155 kb on - strand at 664.155 kb on - strand at 664.159 kb on - strand at 664.159 kb on - strand at 664.188 kb on - strand, within BT0540 at 664.251 kb on - strand, within BT0540 at 664.251 kb on - strand, within BT0540 at 664.263 kb on - strand, within BT0540 at 664.411 kb on + strand, within BT0540 at 664.429 kb on - strand, within BT0540 at 664.572 kb on + strand, within BT0540 at 664.582 kb on + strand, within BT0540 at 664.627 kb on - strand, within BT0540 at 664.687 kb on - strand, within BT0540 at 664.756 kb on - strand, within BT0540 at 664.765 kb on + strand, within BT0540 at 664.995 kb on + strand at 665.006 kb on - strand at 665.196 kb on - strand at 665.285 kb on - strand, within BT0541 at 665.351 kb on - strand, within BT0541 at 665.368 kb on + strand, within BT0541 at 665.420 kb on + strand, within BT0541 at 665.420 kb on + strand, within BT0541 at 665.484 kb on + strand, within BT0541 at 665.489 kb on - strand, within BT0541 at 665.489 kb on - strand, within BT0541 at 665.540 kb on + strand, within BT0541 at 665.636 kb on - strand, within BT0541 at 665.708 kb on - strand, within BT0541 at 665.708 kb on - strand, within BT0541 at 665.708 kb on - strand, within BT0541 at 665.773 kb on + strand, within BT0541 at 665.773 kb on + strand, within BT0541 at 665.818 kb on + strand, within BT0541 at 666.116 kb on + strand, within BT0541 at 666.208 kb on - strand, within BT0541 at 666.272 kb on + strand, within BT0541 at 666.298 kb on - strand, within BT0541 at 666.359 kb on + strand, within BT0541 at 666.421 kb on - strand, within BT0541 at 666.502 kb on - strand, within BT0541 at 666.533 kb on - strand, within BT0541 at 666.543 kb on + strand, within BT0541 at 666.544 kb on - strand, within BT0541 at 666.635 kb on - strand, within BT0541 at 666.855 kb on - strand, within BT0541 at 666.897 kb on - strand, within BT0541 at 666.935 kb on - strand at 666.976 kb on - strand at 666.976 kb on - strand at 667.011 kb on - strand at 667.220 kb on + strand at 667.228 kb on + strand at 667.239 kb on - strand at 667.284 kb on + strand at 667.302 kb on + strand at 667.377 kb on + strand, within BT0542 at 667.575 kb on - strand, within BT0542 at 667.718 kb on + strand, within BT0542 at 667.718 kb on + strand, within BT0542 at 667.719 kb on - strand, within BT0542 at 667.719 kb on - strand, within BT0542 at 667.839 kb on - strand at 667.858 kb on + strand at 667.947 kb on + strand at 667.947 kb on + strand at 667.964 kb on - strand at 667.994 kb on - strand at 668.065 kb on - strand at 668.065 kb on - strand at 668.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 664,100 + -3.2 664,155 - -2.2 664,155 - +1.0 664,159 - -1.0 664,159 - +0.5 664,188 - BT0540 0.12 +2.1 664,251 - BT0540 0.19 +1.5 664,251 - BT0540 0.19 -1.0 664,263 - BT0540 0.21 +1.2 664,411 + BT0540 0.37 -0.3 664,429 - BT0540 0.39 +2.0 664,572 + BT0540 0.54 -0.4 664,582 + BT0540 0.55 -1.8 664,627 - BT0540 0.60 +0.7 664,687 - BT0540 0.67 +1.5 664,756 - BT0540 0.74 +1.5 664,765 + BT0540 0.75 +1.0 664,995 + -2.1 665,006 - -3.2 665,196 - -1.2 665,285 - BT0541 0.14 -2.7 665,351 - BT0541 0.17 -0.2 665,368 + BT0541 0.18 -2.5 665,420 + BT0541 0.20 -2.1 665,420 + BT0541 0.20 +0.3 665,484 + BT0541 0.23 -0.2 665,489 - BT0541 0.23 -1.1 665,489 - BT0541 0.23 -1.9 665,540 + BT0541 0.26 -2.5 665,636 - BT0541 0.30 -1.4 665,708 - BT0541 0.34 -1.6 665,708 - BT0541 0.34 +0.5 665,708 - BT0541 0.34 -0.5 665,773 + BT0541 0.37 -1.6 665,773 + BT0541 0.37 +0.5 665,818 + BT0541 0.39 -1.1 666,116 + BT0541 0.53 -0.5 666,208 - BT0541 0.57 -1.9 666,272 + BT0541 0.60 +2.5 666,298 - BT0541 0.61 -4.2 666,359 + BT0541 0.64 -1.7 666,421 - BT0541 0.67 -1.9 666,502 - BT0541 0.71 -2.5 666,533 - BT0541 0.73 -3.1 666,543 + BT0541 0.73 -1.7 666,544 - BT0541 0.73 -2.5 666,635 - BT0541 0.77 -2.2 666,855 - BT0541 0.88 +0.7 666,897 - BT0541 0.90 -1.7 666,935 - +0.8 666,976 - +0.1 666,976 - -0.9 667,011 - -2.1 667,220 + +0.2 667,228 + -0.9 667,239 - -1.7 667,284 + +1.4 667,302 + -1.4 667,377 + BT0542 0.16 -2.1 667,575 - BT0542 0.50 -0.1 667,718 + BT0542 0.74 -2.8 667,718 + BT0542 0.74 -2.5 667,719 - BT0542 0.75 -2.6 667,719 - BT0542 0.75 -0.3 667,839 - +2.4 667,858 + +1.0 667,947 + +1.6 667,947 + -3.9 667,964 - -1.5 667,994 - +0.4 668,065 - -1.4 668,065 - +1.4 668,101 - +0.3
Or see this region's nucleotide sequence