Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0475 and BT0476 are separated by 4 nucleotides BT0476 and BT0477 are separated by 15 nucleotides BT0477 and BT0478 overlap by 4 nucleotides
BT0475: BT0475 - putative phosphoheptose isomerase (NCBI ptt file), at 586,780 to 587,373
BT0475
BT0476: BT0476 - D-mannose-1-phosphate guanyltransferase (NCBI ptt file), at 587,378 to 588,085
BT0476
BT0477: BT0477 - putative phosphatase (NCBI ptt file), at 588,101 to 588,571
BT0477
BT0478: BT0478 - conserved hypothetical protein (NCBI ptt file), at 588,568 to 589,674
BT0478
Position (kb) 588
589 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 587.188 kb on - strand, within BT0475 at 587.323 kb on - strand at 587.439 kb on + strand at 587.488 kb on - strand, within BT0476 at 587.607 kb on - strand, within BT0476 at 587.891 kb on - strand, within BT0476 at 587.893 kb on - strand, within BT0476 at 587.895 kb on - strand, within BT0476 at 587.895 kb on - strand, within BT0476 at 588.148 kb on - strand, within BT0477 at 588.337 kb on - strand, within BT0477 at 588.337 kb on - strand, within BT0477 at 588.446 kb on - strand, within BT0477 at 588.524 kb on - strand, within BT0477 at 588.536 kb on - strand at 588.695 kb on - strand, within BT0478 at 588.811 kb on - strand, within BT0478 at 588.901 kb on - strand, within BT0478 at 588.926 kb on - strand, within BT0478 at 589.005 kb on - strand, within BT0478 at 589.020 kb on + strand, within BT0478 at 589.020 kb on + strand, within BT0478 at 589.141 kb on - strand, within BT0478 at 589.256 kb on + strand, within BT0478 at 589.379 kb on - strand, within BT0478 at 589.381 kb on - strand, within BT0478 at 589.452 kb on - strand, within BT0478
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 587,188 - BT0475 0.69 -1.7 587,323 - +0.5 587,439 + -2.8 587,488 - BT0476 0.16 +1.1 587,607 - BT0476 0.32 -1.3 587,891 - BT0476 0.72 -2.7 587,893 - BT0476 0.73 +0.5 587,895 - BT0476 0.73 -2.1 587,895 - BT0476 0.73 +1.1 588,148 - BT0477 0.10 +1.2 588,337 - BT0477 0.50 -0.5 588,337 - BT0477 0.50 -1.8 588,446 - BT0477 0.73 -1.8 588,524 - BT0477 0.90 -1.7 588,536 - +0.3 588,695 - BT0478 0.11 +0.1 588,811 - BT0478 0.22 -0.7 588,901 - BT0478 0.30 +0.5 588,926 - BT0478 0.32 -2.1 589,005 - BT0478 0.39 -2.3 589,020 + BT0478 0.41 -0.5 589,020 + BT0478 0.41 -0.4 589,141 - BT0478 0.52 -0.1 589,256 + BT0478 0.62 -1.1 589,379 - BT0478 0.73 +0.3 589,381 - BT0478 0.73 -1.6 589,452 - BT0478 0.80 +0.5
Or see this region's nucleotide sequence