Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0462 and BT0463 are separated by 174 nucleotides BT0463 and BT0464 are separated by 30 nucleotides BT0464 and BT0465 are separated by 3 nucleotides
BT0462: BT0462 - putative transcriptional regulator (NCBI ptt file), at 573,167 to 573,721
BT0462
BT0463: BT0463 - glucose-1-phosphate thymidylyltransferase (NCBI ptt file), at 573,896 to 574,783
BT0463
BT0464: BT0464 - dTDP-4-dehydrorhamnose 3,5-epimerase (NCBI ptt file), at 574,814 to 575,383
BT0464
BT0465: BT0465 - dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 575,387 to 576,241
BT0465
Position (kb) 573
574
575 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 572.948 kb on + strand at 572.978 kb on - strand at 573.039 kb on + strand at 573.039 kb on + strand at 573.125 kb on + strand at 573.216 kb on - strand at 573.239 kb on - strand, within BT0462 at 573.239 kb on - strand, within BT0462 at 573.285 kb on - strand, within BT0462 at 573.405 kb on + strand, within BT0462 at 573.424 kb on + strand, within BT0462 at 573.430 kb on - strand, within BT0462 at 573.633 kb on - strand at 573.645 kb on - strand, within BT0462 at 573.658 kb on + strand, within BT0462 at 573.659 kb on - strand, within BT0462 at 573.670 kb on + strand at 573.880 kb on + strand at 573.977 kb on - strand at 573.999 kb on - strand, within BT0463 at 574.031 kb on - strand, within BT0463 at 574.062 kb on - strand, within BT0463 at 574.288 kb on + strand, within BT0463 at 574.352 kb on - strand, within BT0463 at 574.430 kb on + strand, within BT0463 at 574.435 kb on + strand, within BT0463 at 574.462 kb on + strand, within BT0463 at 574.549 kb on - strand, within BT0463 at 574.549 kb on - strand, within BT0463 at 574.664 kb on - strand, within BT0463 at 574.691 kb on - strand, within BT0463 at 574.720 kb on + strand at 574.746 kb on - strand at 574.791 kb on - strand at 574.823 kb on - strand at 574.840 kb on - strand at 574.948 kb on - strand, within BT0464 at 574.964 kb on - strand, within BT0464 at 574.964 kb on - strand, within BT0464 at 574.992 kb on + strand, within BT0464 at 574.992 kb on + strand, within BT0464 at 575.041 kb on + strand, within BT0464 at 575.107 kb on - strand, within BT0464 at 575.107 kb on - strand, within BT0464 at 575.107 kb on - strand, within BT0464 at 575.128 kb on + strand, within BT0464 at 575.376 kb on + strand at 575.410 kb on + strand at 575.461 kb on + strand at 575.470 kb on - strand at 575.527 kb on - strand, within BT0465 at 575.547 kb on + strand, within BT0465 at 575.582 kb on - strand, within BT0465 at 575.624 kb on - strand, within BT0465 at 575.680 kb on + strand, within BT0465 at 575.710 kb on + strand, within BT0465 at 575.716 kb on + strand, within BT0465 at 575.744 kb on - strand, within BT0465
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 572,948 + -1.6 572,978 - +0.5 573,039 + -0.4 573,039 + +1.0 573,125 + -2.6 573,216 - +1.4 573,239 - BT0462 0.13 +1.2 573,239 - BT0462 0.13 -2.1 573,285 - BT0462 0.21 -1.5 573,405 + BT0462 0.43 -0.9 573,424 + BT0462 0.46 +0.8 573,430 - BT0462 0.47 -0.5 573,633 - +1.5 573,645 - BT0462 0.86 -3.1 573,658 + BT0462 0.88 -1.3 573,659 - BT0462 0.89 +0.5 573,670 + +3.8 573,880 + +1.7 573,977 - -0.5 573,999 - BT0463 0.12 -1.8 574,031 - BT0463 0.15 -1.9 574,062 - BT0463 0.19 -2.5 574,288 + BT0463 0.44 +1.5 574,352 - BT0463 0.51 -0.8 574,430 + BT0463 0.60 -2.9 574,435 + BT0463 0.61 +0.2 574,462 + BT0463 0.64 -0.8 574,549 - BT0463 0.74 -2.5 574,549 - BT0463 0.74 -3.5 574,664 - BT0463 0.86 -1.5 574,691 - BT0463 0.90 +3.5 574,720 + +1.1 574,746 - -1.8 574,791 - -0.3 574,823 - +1.4 574,840 - +0.9 574,948 - BT0464 0.24 -0.8 574,964 - BT0464 0.26 -1.6 574,964 - BT0464 0.26 +1.2 574,992 + BT0464 0.31 +1.2 574,992 + BT0464 0.31 +0.5 575,041 + BT0464 0.40 +0.2 575,107 - BT0464 0.51 +1.4 575,107 - BT0464 0.51 -1.1 575,107 - BT0464 0.51 -3.6 575,128 + BT0464 0.55 -1.3 575,376 + -1.5 575,410 + +2.5 575,461 + -2.4 575,470 - +1.0 575,527 - BT0465 0.16 -2.7 575,547 + BT0465 0.19 -0.8 575,582 - BT0465 0.23 -1.7 575,624 - BT0465 0.28 +0.4 575,680 + BT0465 0.34 -0.3 575,710 + BT0465 0.38 -2.4 575,716 + BT0465 0.38 -0.1 575,744 - BT0465 0.42 -2.4
Or see this region's nucleotide sequence