Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0434 and BT0435 are separated by 38 nucleotides BT0435 and BT0436 are separated by 5 nucleotides
BT0434: BT0434 - conserved hypothetical protein (NCBI ptt file), at 521,967 to 523,850
BT0434
BT0435: BT0435 - hypothetical protein (NCBI ptt file), at 523,889 to 524,989
BT0435
BT0436: BT0436 - arabinose-proton symporter (NCBI ptt file), at 524,995 to 526,401
BT0436
Position (kb)
523
524
525 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 522.927 kb on - strand, within BT0434 at 522.930 kb on - strand, within BT0434 at 522.992 kb on + strand, within BT0434 at 522.993 kb on - strand, within BT0434 at 523.029 kb on - strand, within BT0434 at 523.056 kb on - strand, within BT0434 at 523.080 kb on - strand, within BT0434 at 523.196 kb on + strand, within BT0434 at 523.295 kb on - strand, within BT0434 at 523.358 kb on - strand, within BT0434 at 523.695 kb on - strand at 523.796 kb on - strand at 523.859 kb on - strand at 523.898 kb on - strand at 524.252 kb on - strand, within BT0435 at 524.280 kb on - strand, within BT0435 at 524.316 kb on - strand, within BT0435 at 524.362 kb on - strand, within BT0435 at 524.486 kb on - strand, within BT0435 at 524.885 kb on - strand at 525.025 kb on - strand at 525.085 kb on - strand at 525.165 kb on - strand, within BT0436 at 525.259 kb on - strand, within BT0436 at 525.270 kb on - strand, within BT0436 at 525.311 kb on - strand, within BT0436 at 525.316 kb on - strand, within BT0436 at 525.391 kb on + strand, within BT0436 at 525.505 kb on - strand, within BT0436 at 525.842 kb on + strand, within BT0436 at 525.847 kb on + strand, within BT0436 at 525.848 kb on - strand, within BT0436
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 522,927 - BT0434 0.51 +0.3 522,930 - BT0434 0.51 -1.1 522,992 + BT0434 0.54 +0.9 522,993 - BT0434 0.54 -0.1 523,029 - BT0434 0.56 -1.6 523,056 - BT0434 0.58 +0.7 523,080 - BT0434 0.59 -0.3 523,196 + BT0434 0.65 -0.3 523,295 - BT0434 0.70 -1.5 523,358 - BT0434 0.74 +1.2 523,695 - -1.1 523,796 - -2.7 523,859 - +0.1 523,898 - +0.4 524,252 - BT0435 0.33 -0.8 524,280 - BT0435 0.36 -0.2 524,316 - BT0435 0.39 -0.1 524,362 - BT0435 0.43 +1.6 524,486 - BT0435 0.54 +2.8 524,885 - +0.1 525,025 - -0.1 525,085 - -0.5 525,165 - BT0436 0.12 -0.6 525,259 - BT0436 0.19 +0.4 525,270 - BT0436 0.20 -3.3 525,311 - BT0436 0.22 +0.8 525,316 - BT0436 0.23 -0.5 525,391 + BT0436 0.28 -1.8 525,505 - BT0436 0.36 +3.5 525,842 + BT0436 0.60 +1.6 525,847 + BT0436 0.61 -2.2 525,848 - BT0436 0.61 -0.8
Or see this region's nucleotide sequence