Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt p5482_18 and p5482_19 are separated by 4 nucleotides p5482_19 and p5482_20 are separated by 18 nucleotides p5482_20 and p5482_21 are separated by 556 nucleotides p5482_21 and p5482_22 are separated by 82 nucleotides
p5482_18: p5482_18 - hypothetical protein (NCBI), at 16,898 to 17,605
_18
p5482_19: p5482_19 - hypothetical protein (NCBI), at 17,610 to 18,116
_19
p5482_20: p5482_20 - hypothetical protein (NCBI), at 18,135 to 18,590
_20
p5482_21: p5482_21 - hypothetical protein (NCBI), at 19,147 to 19,419
_21
p5482_22: p5482_22 - hypothetical protein (NCBI), at 19,502 to 19,945
_22
Position (kb) 18
19 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 17.145 kb on - strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.308 kb on - strand, within p5482_18 at 17.308 kb on - strand, within p5482_18 at 17.308 kb on - strand, within p5482_18 at 17.313 kb on + strand, within p5482_18 at 17.313 kb on + strand, within p5482_18 at 17.313 kb on + strand, within p5482_18 at 17.314 kb on - strand, within p5482_18 at 17.314 kb on - strand, within p5482_18 at 17.314 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.367 kb on - strand, within p5482_18 at 17.438 kb on + strand, within p5482_18 at 17.440 kb on + strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.583 kb on + strand at 17.583 kb on + strand at 17.583 kb on + strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.590 kb on - strand at 17.590 kb on - strand at 17.596 kb on - strand at 17.610 kb on - strand at 17.629 kb on + strand at 17.629 kb on + strand at 17.670 kb on + strand, within p5482_19 at 17.670 kb on + strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.685 kb on + strand, within p5482_19 at 17.685 kb on + strand, within p5482_19 at 17.686 kb on - strand, within p5482_19 at 17.687 kb on + strand, within p5482_19 at 17.687 kb on + strand, within p5482_19 at 17.688 kb on - strand, within p5482_19 at 17.706 kb on + strand, within p5482_19 at 17.707 kb on - strand, within p5482_19 at 17.709 kb on - strand, within p5482_19 at 17.709 kb on - strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.758 kb on - strand, within p5482_19 at 17.786 kb on + strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.853 kb on + strand, within p5482_19 at 17.854 kb on - strand, within p5482_19 at 17.856 kb on + strand, within p5482_19 at 17.856 kb on + strand, within p5482_19 at 17.856 kb on + strand, within p5482_19 at 17.857 kb on - strand, within p5482_19 at 17.946 kb on + strand, within p5482_19 at 17.947 kb on - strand, within p5482_19 at 17.947 kb on - strand, within p5482_19 at 17.947 kb on - strand at 17.979 kb on + strand, within p5482_19 at 17.979 kb on + strand, within p5482_19 at 17.980 kb on - strand, within p5482_19 at 18.011 kb on - strand, within p5482_19 at 18.059 kb on + strand, within p5482_19 at 18.059 kb on + strand, within p5482_19 at 18.060 kb on - strand, within p5482_19 at 18.077 kb on + strand at 18.077 kb on + strand at 18.081 kb on + strand at 18.109 kb on - strand at 18.112 kb on + strand at 18.113 kb on - strand at 18.113 kb on - strand at 18.115 kb on - strand at 18.121 kb on - strand at 18.124 kb on + strand at 18.134 kb on + strand at 18.135 kb on - strand at 18.144 kb on + strand at 18.145 kb on - strand at 18.145 kb on - strand at 18.150 kb on + strand at 18.151 kb on - strand at 18.151 kb on - strand at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.245 kb on - strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.282 kb on - strand, within p5482_20 at 18.295 kb on + strand, within p5482_20 at 18.308 kb on + strand, within p5482_20 at 18.362 kb on - strand, within p5482_20 at 18.362 kb on - strand, within p5482_20 at 18.397 kb on + strand, within p5482_20 at 18.556 kb on + strand at 18.556 kb on + strand at 18.556 kb on + strand at 18.556 kb on + strand at 18.557 kb on - strand at 18.557 kb on - strand at 18.557 kb on - strand at 18.560 kb on + strand at 18.560 kb on + strand at 18.560 kb on + strand at 18.572 kb on + strand at 18.579 kb on + strand at 18.582 kb on + strand at 18.583 kb on - strand at 18.583 kb on - strand at 18.583 kb on - strand at 18.584 kb on + strand at 18.585 kb on - strand at 18.585 kb on - strand at 18.672 kb on - strand at 18.672 kb on - strand at 18.706 kb on + strand at 18.707 kb on - strand at 18.707 kb on - strand at 18.841 kb on - strand at 18.847 kb on + strand at 18.847 kb on + strand at 18.875 kb on + strand at 18.875 kb on + strand at 18.875 kb on + strand at 18.876 kb on - strand at 18.876 kb on - strand at 18.876 kb on - strand at 18.879 kb on + strand at 18.879 kb on + strand at 18.879 kb on + strand at 18.880 kb on - strand at 18.928 kb on + strand at 18.929 kb on - strand at 18.941 kb on + strand at 18.942 kb on - strand at 18.968 kb on + strand at 18.969 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.990 kb on + strand at 18.990 kb on + strand at 18.991 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 19.043 kb on + strand at 19.044 kb on - strand at 19.086 kb on + strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.091 kb on + strand at 19.095 kb on + strand at 19.104 kb on - strand at 19.124 kb on + strand at 19.124 kb on + strand at 19.127 kb on + strand at 19.128 kb on - strand at 19.136 kb on + strand at 19.136 kb on + strand at 19.146 kb on + strand at 19.158 kb on + strand at 19.158 kb on + strand at 19.159 kb on - strand at 19.159 kb on - strand at 19.222 kb on + strand, within p5482_21 at 19.222 kb on + strand, within p5482_21 at 19.224 kb on + strand, within p5482_21 at 19.224 kb on + strand, within p5482_21 at 19.225 kb on - strand, within p5482_21 at 19.238 kb on + strand, within p5482_21 at 19.342 kb on + strand, within p5482_21 at 19.342 kb on + strand, within p5482_21 at 19.343 kb on - strand, within p5482_21 at 19.356 kb on + strand, within p5482_21 at 19.357 kb on - strand, within p5482_21 at 19.450 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.531 kb on - strand at 19.558 kb on + strand, within p5482_22 at 19.558 kb on + strand, within p5482_22
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 17,145 - p5482_18 0.35 +1.0 17,231 + p5482_18 0.47 -2.3 17,231 + p5482_18 0.47 +1.0 17,231 + p5482_18 0.47 +0.7 17,231 + p5482_18 0.47 +0.4 17,231 + p5482_18 0.47 -1.9 17,232 - p5482_18 0.47 -2.7 17,232 - p5482_18 0.47 +1.2 17,232 - p5482_18 0.47 +0.3 17,232 - +0.4 17,232 - p5482_18 0.47 +0.6 17,232 - p5482_18 0.47 -2.0 17,232 - p5482_18 0.47 -4.6 17,232 - p5482_18 0.47 +0.9 17,232 - p5482_18 0.47 +1.4 17,308 - p5482_18 0.58 +4.6 17,308 - p5482_18 0.58 +1.7 17,308 - p5482_18 0.58 +0.8 17,313 + p5482_18 0.59 -2.1 17,313 + p5482_18 0.59 -3.9 17,313 + p5482_18 0.59 +0.6 17,314 - p5482_18 0.59 -0.2 17,314 - p5482_18 0.59 +0.2 17,314 - p5482_18 0.59 +0.9 17,339 - p5482_18 0.62 +0.8 17,339 - p5482_18 0.62 -2.7 17,339 - p5482_18 0.62 +0.7 17,339 - p5482_18 0.62 -2.1 17,367 - p5482_18 0.66 -1.1 17,438 + p5482_18 0.76 -3.5 17,440 + p5482_18 0.77 -0.6 17,441 - p5482_18 0.77 -0.7 17,441 - p5482_18 0.77 +1.0 17,441 - p5482_18 0.77 -1.9 17,441 - p5482_18 0.77 +0.2 17,583 + -1.2 17,583 + +0.6 17,583 + -2.6 17,584 - -1.0 17,584 - +1.0 17,584 - +0.5 17,584 - +0.4 17,584 - +0.5 17,584 - -1.6 17,590 - +0.4 17,590 - -4.6 17,596 - +1.0 17,610 - +1.1 17,629 + +0.9 17,629 + +2.6 17,670 + p5482_19 0.12 +0.8 17,670 + p5482_19 0.12 +0.7 17,671 - p5482_19 0.12 +0.4 17,671 - p5482_19 0.12 -3.7 17,671 - p5482_19 0.12 -2.1 17,671 - p5482_19 0.12 -2.6 17,671 - p5482_19 0.12 +0.6 17,685 + p5482_19 0.15 +2.6 17,685 + p5482_19 0.15 +0.7 17,686 - p5482_19 0.15 +2.0 17,687 + p5482_19 0.15 +1.2 17,687 + p5482_19 0.15 -2.3 17,688 - p5482_19 0.15 -2.4 17,706 + p5482_19 0.19 -0.4 17,707 - p5482_19 0.19 +1.2 17,709 - p5482_19 0.20 -3.7 17,709 - p5482_19 0.20 -3.5 17,757 + p5482_19 0.29 +0.8 17,757 + p5482_19 0.29 -1.7 17,757 + p5482_19 0.29 +0.6 17,757 + p5482_19 0.29 +1.3 17,758 - p5482_19 0.29 -1.6 17,786 + p5482_19 0.35 +0.7 17,787 - p5482_19 0.35 +0.6 17,787 - p5482_19 0.35 -2.5 17,787 - p5482_19 0.35 +0.5 17,787 - p5482_19 0.35 -0.1 17,787 - p5482_19 0.35 +0.5 17,853 + p5482_19 0.48 +1.2 17,854 - p5482_19 0.48 -2.4 17,856 + p5482_19 0.49 -2.2 17,856 + p5482_19 0.49 +1.9 17,856 + p5482_19 0.49 -3.1 17,857 - p5482_19 0.49 +0.4 17,946 + p5482_19 0.66 +1.0 17,947 - p5482_19 0.66 +0.4 17,947 - p5482_19 0.66 -1.8 17,947 - +0.4 17,979 + p5482_19 0.73 +0.9 17,979 + p5482_19 0.73 -1.6 17,980 - p5482_19 0.73 -1.1 18,011 - p5482_19 0.79 +2.4 18,059 + p5482_19 0.89 +1.3 18,059 + p5482_19 0.89 -0.6 18,060 - p5482_19 0.89 +0.2 18,077 + +0.7 18,077 + -3.4 18,081 + -3.4 18,109 - -0.9 18,112 + +0.9 18,113 - +1.1 18,113 - -0.6 18,115 - -0.6 18,121 - +0.4 18,124 + -2.2 18,134 + +1.6 18,135 - +1.2 18,144 + +0.4 18,145 - -0.6 18,145 - +2.0 18,150 + -1.9 18,151 - +0.8 18,151 - +0.1 18,237 + p5482_20 0.22 +0.4 18,237 + p5482_20 0.22 +0.4 18,237 + p5482_20 0.22 -1.4 18,237 + p5482_20 0.22 -2.4 18,237 + p5482_20 0.22 +0.4 18,237 + p5482_20 0.22 -0.6 18,238 - p5482_20 0.23 -2.3 18,238 - p5482_20 0.23 -0.8 18,238 - p5482_20 0.23 -2.1 18,238 - p5482_20 0.23 +1.4 18,245 - p5482_20 0.24 -3.5 18,281 + p5482_20 0.32 -2.7 18,281 + p5482_20 0.32 +0.7 18,281 + p5482_20 0.32 +0.7 18,281 + p5482_20 0.32 +0.8 18,282 - p5482_20 0.32 -2.4 18,295 + p5482_20 0.35 -2.4 18,308 + p5482_20 0.38 +0.9 18,362 - p5482_20 0.50 -2.0 18,362 - p5482_20 0.50 -1.8 18,397 + p5482_20 0.57 -1.6 18,556 + -1.2 18,556 + +2.1 18,556 + +0.4 18,556 + -2.3 18,557 - -3.7 18,557 - +1.1 18,557 - +1.0 18,560 + +2.1 18,560 + -1.6 18,560 + +0.9 18,572 + +1.2 18,579 + -0.8 18,582 + +1.0 18,583 - -1.1 18,583 - +0.9 18,583 - -0.7 18,584 + -2.4 18,585 - +1.4 18,585 - -4.1 18,672 - -2.6 18,672 - +1.4 18,706 + -2.2 18,707 - +0.3 18,707 - -2.7 18,841 - -4.0 18,847 + -2.2 18,847 + +0.6 18,875 + +1.3 18,875 + +0.4 18,875 + +1.0 18,876 - +0.8 18,876 - -0.6 18,876 - -4.3 18,879 + -0.0 18,879 + -2.6 18,879 + -0.3 18,880 - +0.1 18,928 + +1.8 18,929 - -0.9 18,941 + +1.1 18,942 - -0.7 18,968 + -0.9 18,969 - -3.3 18,977 - +0.4 18,977 - -0.6 18,977 - -1.2 18,990 + -2.2 18,990 + -1.7 18,991 - +1.5 18,995 - -2.4 18,995 - -2.7 18,995 - +1.1 18,995 - +1.9 19,043 + -2.4 19,044 - -0.9 19,086 + -2.7 19,087 - -0.2 19,087 - -0.6 19,087 - +0.4 19,087 - +0.8 19,091 + -1.1 19,095 + +1.7 19,104 - +0.5 19,124 + -4.4 19,124 + -2.5 19,127 + -2.4 19,128 - +1.1 19,136 + +1.4 19,136 + +0.9 19,146 + +2.7 19,158 + -4.6 19,158 + +2.0 19,159 - -1.5 19,159 - +1.2 19,222 + p5482_21 0.27 +1.1 19,222 + p5482_21 0.27 +1.3 19,224 + p5482_21 0.28 +0.4 19,224 + p5482_21 0.28 +1.3 19,225 - p5482_21 0.29 +0.4 19,238 + p5482_21 0.33 +1.2 19,342 + p5482_21 0.71 -3.7 19,342 + p5482_21 0.71 -1.0 19,343 - p5482_21 0.72 -1.7 19,356 + p5482_21 0.77 -1.0 19,357 - p5482_21 0.77 +2.6 19,450 + +1.1 19,530 + -1.4 19,530 + -2.6 19,530 + -2.9 19,530 + -3.1 19,530 + +1.3 19,531 - -1.2 19,558 + p5482_22 0.13 +0.7 19,558 + p5482_22 0.13 +1.1
Or see this region's nucleotide sequence