Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt p5482_14 and p5482_15 are separated by 4 nucleotides p5482_15 and p5482_16 overlap by 4 nucleotides p5482_16 and p5482_17 are separated by 536 nucleotides p5482_17 and p5482_18 are separated by 10 nucleotides
p5482_14: p5482_14 - hypothetical protein (NCBI), at 13,508 to 14,152
_14
p5482_15: p5482_15 - TraM-like protein (NCBI), at 14,157 to 15,047
_15
p5482_16: p5482_16 - TraN-like protein (NCBI), at 15,044 to 16,141
_16
p5482_17: p5482_17 - hypothetical protein (NCBI), at 16,678 to 16,887
_17
p5482_18: p5482_18 - hypothetical protein (NCBI), at 16,898 to 17,605
_18
Position (kb) 15
16
17 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 14.108 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.128 kb on - strand at 14.151 kb on - strand at 14.157 kb on - strand at 14.161 kb on - strand at 14.295 kb on - strand, within p5482_15 at 14.295 kb on - strand, within p5482_15 at 14.331 kb on - strand, within p5482_15 at 14.382 kb on + strand, within p5482_15 at 14.403 kb on - strand, within p5482_15 at 14.406 kb on + strand, within p5482_15 at 14.407 kb on - strand, within p5482_15 at 14.429 kb on - strand, within p5482_15 at 14.446 kb on + strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.560 kb on + strand, within p5482_15 at 14.561 kb on - strand, within p5482_15 at 14.657 kb on - strand, within p5482_15 at 14.705 kb on + strand, within p5482_15 at 14.706 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.761 kb on + strand, within p5482_15 at 14.796 kb on + strand, within p5482_15 at 14.797 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.844 kb on + strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.916 kb on - strand, within p5482_15 at 14.923 kb on - strand, within p5482_15 at 14.939 kb on - strand, within p5482_15 at 14.943 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 15.028 kb on - strand at 15.029 kb on + strand at 15.030 kb on - strand at 15.034 kb on + strand at 15.034 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.053 kb on - strand at 15.059 kb on - strand at 15.124 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.151 kb on - strand at 15.159 kb on + strand, within p5482_16 at 15.159 kb on + strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.323 kb on - strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.401 kb on + strand, within p5482_16 at 15.452 kb on + strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.455 kb on - strand, within p5482_16 at 15.457 kb on - strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.649 kb on - strand, within p5482_16 at 15.738 kb on + strand, within p5482_16 at 15.739 kb on - strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.743 kb on - strand at 15.815 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.878 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.884 kb on + strand, within p5482_16 at 15.885 kb on - strand, within p5482_16 at 15.887 kb on - strand, within p5482_16 at 15.897 kb on - strand, within p5482_16 at 16.025 kb on - strand, within p5482_16 at 16.139 kb on + strand at 16.140 kb on - strand at 16.140 kb on - strand at 16.140 kb on - strand at 16.234 kb on - strand at 16.281 kb on - strand at 16.324 kb on - strand at 16.424 kb on - strand at 16.487 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.543 kb on - strand at 16.610 kb on + strand at 16.630 kb on - strand at 16.630 kb on - strand at 16.640 kb on - strand at 16.657 kb on + strand at 16.666 kb on + strand at 16.667 kb on - strand at 16.686 kb on - strand at 16.686 kb on - strand at 16.686 kb on - strand at 16.689 kb on + strand at 16.689 kb on + strand at 16.703 kb on + strand, within p5482_17 at 16.703 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.817 kb on - strand, within p5482_17 at 16.869 kb on + strand at 16.870 kb on - strand at 16.886 kb on - strand at 16.890 kb on + strand at 16.898 kb on - strand at 16.909 kb on - strand at 16.999 kb on + strand, within p5482_18 at 16.999 kb on + strand, within p5482_18 at 17.000 kb on - strand, within p5482_18 at 17.001 kb on + strand, within p5482_18 at 17.019 kb on + strand, within p5482_18 at 17.047 kb on + strand, within p5482_18 at 17.047 kb on + strand, within p5482_18 at 17.048 kb on - strand, within p5482_18 at 17.085 kb on + strand, within p5482_18 at 17.085 kb on + strand, within p5482_18 at 17.086 kb on - strand, within p5482_18 at 17.086 kb on - strand, within p5482_18
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 14,108 - +0.5 14,110 - -1.1 14,110 - +0.4 14,110 - -2.0 14,128 - -1.1 14,151 - -1.9 14,157 - -4.1 14,161 - +1.8 14,295 - p5482_15 0.15 -0.3 14,295 - p5482_15 0.15 +0.4 14,331 - p5482_15 0.20 -1.0 14,382 + p5482_15 0.25 -2.5 14,403 - p5482_15 0.28 -3.4 14,406 + p5482_15 0.28 -2.4 14,407 - p5482_15 0.28 -2.6 14,429 - p5482_15 0.31 -3.0 14,446 + p5482_15 0.32 -4.5 14,447 - p5482_15 0.33 +1.1 14,447 - p5482_15 0.33 -0.6 14,560 + p5482_15 0.45 -2.4 14,561 - p5482_15 0.45 +0.4 14,657 - p5482_15 0.56 +0.6 14,705 + p5482_15 0.62 -4.2 14,706 - p5482_15 0.62 +1.1 14,760 - p5482_15 0.68 +0.1 14,760 - p5482_15 0.68 -2.3 14,760 - p5482_15 0.68 +0.8 14,760 - p5482_15 0.68 +0.4 14,760 - p5482_15 0.68 +0.9 14,761 + p5482_15 0.68 -1.6 14,796 + p5482_15 0.72 -2.9 14,797 - p5482_15 0.72 -2.3 14,841 - p5482_15 0.77 +0.4 14,841 - p5482_15 0.77 +1.0 14,841 - p5482_15 0.77 -1.6 14,841 - p5482_15 0.77 +2.2 14,843 - p5482_15 0.77 +1.1 14,843 - p5482_15 0.77 +0.1 14,843 - p5482_15 0.77 +1.1 14,844 + p5482_15 0.77 -1.8 14,845 - p5482_15 0.77 +1.1 14,845 - p5482_15 0.77 +1.3 14,845 - p5482_15 0.77 +1.1 14,845 - p5482_15 0.77 +0.9 14,845 - p5482_15 0.77 +0.4 14,845 - p5482_15 0.77 +0.8 14,845 - p5482_15 0.77 +1.1 14,845 - p5482_15 0.77 -0.8 14,916 - p5482_15 0.85 -2.0 14,923 - p5482_15 0.86 +1.3 14,939 - p5482_15 0.88 +2.2 14,943 - p5482_15 0.88 -1.2 14,952 - p5482_15 0.89 -1.1 14,952 - p5482_15 0.89 +0.5 15,028 - +1.7 15,029 + -1.6 15,030 - -1.6 15,034 + +0.4 15,034 + -1.4 15,052 + +0.4 15,052 + -5.8 15,052 + -5.5 15,052 + -3.1 15,053 - -0.4 15,059 - +1.4 15,124 + -3.5 15,150 + +0.4 15,150 + -4.1 15,150 + -2.9 15,151 - -2.4 15,159 + p5482_16 0.10 -2.9 15,159 + p5482_16 0.10 -2.4 15,160 - p5482_16 0.11 +0.7 15,160 - p5482_16 0.11 -1.1 15,160 - p5482_16 0.11 -0.0 15,179 + p5482_16 0.12 -3.7 15,179 + p5482_16 0.12 -1.9 15,179 + p5482_16 0.12 -3.9 15,181 + p5482_16 0.12 -3.1 15,181 + p5482_16 0.12 -3.9 15,232 + p5482_16 0.17 +0.4 15,232 + p5482_16 0.17 +0.4 15,232 + p5482_16 0.17 -2.6 15,233 - p5482_16 0.17 +0.8 15,233 - p5482_16 0.17 -3.7 15,323 - p5482_16 0.25 +1.7 15,332 + p5482_16 0.26 -2.7 15,332 + p5482_16 0.26 -1.1 15,333 - p5482_16 0.26 +0.4 15,333 - p5482_16 0.26 -3.4 15,333 - p5482_16 0.26 +1.7 15,333 - p5482_16 0.26 -1.9 15,352 + p5482_16 0.28 -2.1 15,352 + p5482_16 0.28 -1.1 15,352 + p5482_16 0.28 -3.9 15,352 + p5482_16 0.28 -4.2 15,353 - p5482_16 0.28 -2.0 15,353 - p5482_16 0.28 +0.8 15,373 + p5482_16 0.30 -3.3 15,373 + p5482_16 0.30 -5.7 15,384 + p5482_16 0.31 -3.3 15,384 + p5482_16 0.31 -2.1 15,384 + p5482_16 0.31 -2.6 15,385 - p5482_16 0.31 -1.1 15,385 - p5482_16 0.31 +0.4 15,385 - p5482_16 0.31 -1.1 15,397 + p5482_16 0.32 -2.4 15,397 + p5482_16 0.32 -1.9 15,398 - p5482_16 0.32 +1.2 15,398 - p5482_16 0.32 +0.9 15,398 - p5482_16 0.32 +0.7 15,398 - p5482_16 0.32 -1.6 15,398 - p5482_16 0.32 -3.5 15,401 + p5482_16 0.33 -3.2 15,452 + p5482_16 0.37 -4.1 15,453 - p5482_16 0.37 +1.2 15,453 - p5482_16 0.37 -0.3 15,453 - p5482_16 0.37 -3.7 15,453 - p5482_16 0.37 +0.4 15,453 - p5482_16 0.37 +1.6 15,455 - p5482_16 0.37 -2.9 15,457 - p5482_16 0.38 +0.4 15,477 + p5482_16 0.39 -3.0 15,477 + p5482_16 0.39 -0.6 15,483 + p5482_16 0.40 -3.0 15,483 + p5482_16 0.40 -0.6 15,483 + p5482_16 0.40 -2.2 15,484 - p5482_16 0.40 +2.7 15,484 - p5482_16 0.40 -2.0 15,484 - p5482_16 0.40 -3.3 15,484 - p5482_16 0.40 +2.0 15,484 - p5482_16 0.40 -4.0 15,509 - p5482_16 0.42 +0.4 15,509 - p5482_16 0.42 +1.4 15,597 - p5482_16 0.50 +0.3 15,597 - p5482_16 0.50 +1.2 15,649 - p5482_16 0.55 -2.9 15,738 + p5482_16 0.63 +0.4 15,739 - p5482_16 0.63 +1.5 15,740 + p5482_16 0.63 -5.5 15,740 + p5482_16 0.63 -4.0 15,740 + p5482_16 0.63 -3.4 15,741 - p5482_16 0.63 -1.6 15,741 - p5482_16 0.63 -3.8 15,741 - p5482_16 0.63 +0.4 15,741 - p5482_16 0.63 -3.0 15,741 - p5482_16 0.63 +0.8 15,741 - p5482_16 0.63 -3.2 15,743 - -1.6 15,815 - p5482_16 0.70 +0.6 15,817 - p5482_16 0.70 -1.8 15,817 - p5482_16 0.70 +1.4 15,817 - p5482_16 0.70 -2.3 15,817 - p5482_16 0.70 -1.0 15,817 - p5482_16 0.70 -1.7 15,817 - p5482_16 0.70 -1.0 15,819 - p5482_16 0.71 +1.3 15,819 - p5482_16 0.71 +0.8 15,819 - p5482_16 0.71 +2.0 15,873 - p5482_16 0.76 +0.9 15,873 - p5482_16 0.76 -2.4 15,878 - p5482_16 0.76 -2.6 15,880 - p5482_16 0.76 +1.5 15,880 - p5482_16 0.76 -4.0 15,880 - p5482_16 0.76 +1.2 15,880 - p5482_16 0.76 +2.4 15,880 - p5482_16 0.76 +0.4 15,880 - p5482_16 0.76 -1.8 15,884 + p5482_16 0.77 -3.0 15,885 - p5482_16 0.77 -3.1 15,887 - p5482_16 0.77 -0.3 15,897 - p5482_16 0.78 +0.4 16,025 - p5482_16 0.89 +0.5 16,139 + +0.4 16,140 - +1.4 16,140 - -2.7 16,140 - +0.4 16,234 - +1.5 16,281 - +1.4 16,324 - +1.5 16,424 - -0.3 16,487 - +0.7 16,522 - -0.3 16,522 - -3.1 16,522 - -1.7 16,522 - -3.4 16,522 - -0.0 16,543 - +0.1 16,610 + -1.7 16,630 - -0.1 16,630 - -2.6 16,640 - +0.4 16,657 + -3.7 16,666 + -4.0 16,667 - +0.6 16,686 - -1.1 16,686 - +1.9 16,686 - +0.5 16,689 + -3.9 16,689 + -3.0 16,703 + p5482_17 0.12 +1.2 16,703 + p5482_17 0.12 +1.2 16,816 + p5482_17 0.66 +1.4 16,816 + p5482_17 0.66 -3.3 16,816 + p5482_17 0.66 +1.0 16,816 + p5482_17 0.66 +1.0 16,816 + p5482_17 0.66 -3.3 16,816 + p5482_17 0.66 -1.9 16,816 + p5482_17 0.66 -2.3 16,817 - p5482_17 0.66 +1.0 16,869 + +0.9 16,870 - +0.4 16,886 - -2.0 16,890 + -0.3 16,898 - +0.6 16,909 - -0.3 16,999 + p5482_18 0.14 +1.5 16,999 + p5482_18 0.14 +2.5 17,000 - p5482_18 0.14 +2.1 17,001 + p5482_18 0.15 +1.7 17,019 + p5482_18 0.17 +0.8 17,047 + p5482_18 0.21 -3.1 17,047 + p5482_18 0.21 +0.9 17,048 - p5482_18 0.21 +1.4 17,085 + p5482_18 0.26 -2.6 17,085 + p5482_18 0.26 -1.6 17,086 - p5482_18 0.27 +0.4 17,086 - p5482_18 0.27 -3.4
Or see this region's nucleotide sequence