Experiment: R2A with bismuth(III) chloride 0.03125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS11465 and LRK54_RS11470 are separated by 28 nucleotides LRK54_RS11470 and LRK54_RS11475 overlap by 4 nucleotides LRK54_RS11475 and LRK54_RS11480 overlap by 14 nucleotides
LRK54_RS11465: LRK54_RS11465 - serine hydrolase, at 2,543,340 to 2,544,389
_RS11465
LRK54_RS11470: LRK54_RS11470 - M15 family metallopeptidase, at 2,544,418 to 2,545,152
_RS11470
LRK54_RS11475: LRK54_RS11475 - serine hydrolase, at 2,545,149 to 2,546,828
_RS11475
LRK54_RS11480: LRK54_RS11480 - dipeptidase, at 2,546,815 to 2,548,026
_RS11480
Position (kb)
2545
2546
2547 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2544.169 kb on + strand, within LRK54_RS11465 at 2544.220 kb on + strand, within LRK54_RS11465 at 2544.220 kb on + strand, within LRK54_RS11465 at 2544.220 kb on + strand, within LRK54_RS11465 at 2544.220 kb on + strand, within LRK54_RS11465 at 2544.221 kb on - strand, within LRK54_RS11465 at 2544.221 kb on - strand, within LRK54_RS11465 at 2544.221 kb on - strand, within LRK54_RS11465 at 2544.221 kb on - strand, within LRK54_RS11465 at 2544.221 kb on - strand, within LRK54_RS11465 at 2544.290 kb on - strand at 2544.290 kb on - strand at 2544.290 kb on - strand at 2544.437 kb on + strand at 2544.437 kb on + strand at 2544.438 kb on - strand at 2544.438 kb on - strand at 2544.438 kb on - strand at 2544.438 kb on - strand at 2544.438 kb on - strand at 2544.438 kb on - strand at 2544.473 kb on + strand at 2544.473 kb on + strand at 2544.473 kb on + strand at 2544.474 kb on - strand at 2544.474 kb on - strand at 2544.474 kb on - strand at 2544.474 kb on - strand at 2544.474 kb on - strand at 2544.474 kb on - strand at 2544.474 kb on - strand at 2544.495 kb on - strand, within LRK54_RS11470 at 2544.713 kb on + strand, within LRK54_RS11470 at 2544.713 kb on + strand, within LRK54_RS11470 at 2544.713 kb on + strand, within LRK54_RS11470 at 2544.713 kb on + strand, within LRK54_RS11470 at 2544.714 kb on - strand, within LRK54_RS11470 at 2544.714 kb on - strand, within LRK54_RS11470 at 2544.714 kb on - strand, within LRK54_RS11470 at 2544.714 kb on - strand, within LRK54_RS11470 at 2544.714 kb on - strand, within LRK54_RS11470 at 2544.750 kb on - strand, within LRK54_RS11470 at 2544.751 kb on - strand, within LRK54_RS11470 at 2544.933 kb on - strand, within LRK54_RS11470 at 2544.974 kb on + strand, within LRK54_RS11470 at 2544.974 kb on + strand, within LRK54_RS11470 at 2544.974 kb on + strand, within LRK54_RS11470 at 2544.974 kb on + strand, within LRK54_RS11470 at 2544.975 kb on - strand, within LRK54_RS11470 at 2544.975 kb on - strand, within LRK54_RS11470 at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.146 kb on + strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.147 kb on - strand at 2545.192 kb on + strand at 2545.192 kb on + strand at 2545.193 kb on - strand at 2545.315 kb on + strand at 2545.316 kb on - strand at 2545.316 kb on - strand at 2545.430 kb on - strand, within LRK54_RS11475 at 2545.430 kb on - strand, within LRK54_RS11475 at 2545.485 kb on - strand, within LRK54_RS11475 at 2545.485 kb on - strand, within LRK54_RS11475 at 2545.543 kb on + strand, within LRK54_RS11475 at 2545.543 kb on + strand, within LRK54_RS11475 at 2545.543 kb on + strand, within LRK54_RS11475 at 2545.543 kb on + strand, within LRK54_RS11475 at 2545.544 kb on - strand, within LRK54_RS11475 at 2545.544 kb on - strand, within LRK54_RS11475 at 2545.544 kb on - strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.687 kb on + strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.688 kb on - strand, within LRK54_RS11475 at 2545.762 kb on + strand, within LRK54_RS11475 at 2545.763 kb on - strand, within LRK54_RS11475 at 2546.161 kb on + strand, within LRK54_RS11475 at 2546.161 kb on + strand, within LRK54_RS11475 at 2546.161 kb on + strand, within LRK54_RS11475 at 2546.161 kb on + strand, within LRK54_RS11475 at 2546.161 kb on + strand, within LRK54_RS11475 at 2546.161 kb on + strand, within LRK54_RS11475 at 2546.161 kb on + strand, within LRK54_RS11475 at 2546.162 kb on - strand, within LRK54_RS11475 at 2546.162 kb on - strand, within LRK54_RS11475 at 2546.177 kb on - strand, within LRK54_RS11475 at 2546.177 kb on - strand, within LRK54_RS11475 at 2546.177 kb on - strand, within LRK54_RS11475 at 2546.182 kb on + strand, within LRK54_RS11475 at 2546.182 kb on + strand, within LRK54_RS11475 at 2546.183 kb on - strand, within LRK54_RS11475 at 2546.183 kb on - strand, within LRK54_RS11475 at 2546.183 kb on - strand, within LRK54_RS11475 at 2546.183 kb on - strand, within LRK54_RS11475 at 2546.183 kb on - strand, within LRK54_RS11475 at 2546.200 kb on + strand, within LRK54_RS11475 at 2546.377 kb on + strand, within LRK54_RS11475 at 2546.377 kb on + strand, within LRK54_RS11475 at 2546.377 kb on + strand, within LRK54_RS11475 at 2546.377 kb on + strand, within LRK54_RS11475 at 2546.377 kb on + strand, within LRK54_RS11475 at 2546.378 kb on - strand, within LRK54_RS11475 at 2546.378 kb on - strand, within LRK54_RS11475 at 2546.378 kb on - strand, within LRK54_RS11475 at 2546.378 kb on - strand, within LRK54_RS11475 at 2546.536 kb on + strand, within LRK54_RS11475 at 2546.537 kb on - strand, within LRK54_RS11475 at 2546.537 kb on - strand, within LRK54_RS11475 at 2546.537 kb on - strand, within LRK54_RS11475 at 2546.537 kb on - strand, within LRK54_RS11475 at 2546.537 kb on - strand, within LRK54_RS11475 at 2546.587 kb on + strand, within LRK54_RS11475 at 2546.709 kb on + strand at 2546.709 kb on + strand at 2546.709 kb on + strand at 2546.709 kb on + strand at 2546.709 kb on + strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.710 kb on - strand at 2546.790 kb on + strand at 2546.790 kb on + strand at 2546.790 kb on + strand at 2546.790 kb on + strand at 2546.790 kb on + strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2546.791 kb on - strand at 2547.044 kb on + strand, within LRK54_RS11480 at 2547.044 kb on + strand, within LRK54_RS11480 at 2547.045 kb on - strand, within LRK54_RS11480 at 2547.045 kb on - strand, within LRK54_RS11480 at 2547.045 kb on - strand, within LRK54_RS11480 at 2547.045 kb on - strand, within LRK54_RS11480 at 2547.045 kb on - strand, within LRK54_RS11480 at 2547.114 kb on - strand, within LRK54_RS11480 at 2547.134 kb on + strand, within LRK54_RS11480 at 2547.135 kb on - strand, within LRK54_RS11480 at 2547.135 kb on - strand, within LRK54_RS11480 at 2547.135 kb on - strand, within LRK54_RS11480 at 2547.224 kb on + strand, within LRK54_RS11480 at 2547.224 kb on + strand, within LRK54_RS11480 at 2547.225 kb on - strand, within LRK54_RS11480 at 2547.611 kb on + strand, within LRK54_RS11480 at 2547.612 kb on - strand, within LRK54_RS11480 at 2547.612 kb on - strand, within LRK54_RS11480 at 2547.707 kb on + strand, within LRK54_RS11480 at 2547.707 kb on + strand, within LRK54_RS11480 at 2547.707 kb on + strand, within LRK54_RS11480 at 2547.707 kb on + strand, within LRK54_RS11480 at 2547.707 kb on + strand, within LRK54_RS11480 at 2547.707 kb on + strand, within LRK54_RS11480 at 2547.708 kb on - strand, within LRK54_RS11480 at 2547.708 kb on - strand, within LRK54_RS11480 at 2547.708 kb on - strand, within LRK54_RS11480 at 2547.708 kb on - strand, within LRK54_RS11480 at 2547.708 kb on - strand, within LRK54_RS11480 at 2547.708 kb on - strand, within LRK54_RS11480 at 2547.708 kb on - strand, within LRK54_RS11480 at 2547.737 kb on + strand, within LRK54_RS11480 at 2547.737 kb on + strand, within LRK54_RS11480 at 2547.738 kb on - strand, within LRK54_RS11480 at 2547.738 kb on - strand, within LRK54_RS11480 at 2547.771 kb on - strand, within LRK54_RS11480
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with bismuth(III) chloride 0.03125 mM remove 2,544,169 + LRK54_RS11465 0.79 +2.0 2,544,220 + LRK54_RS11465 0.84 +0.5 2,544,220 + LRK54_RS11465 0.84 +0.4 2,544,220 + LRK54_RS11465 0.84 -1.7 2,544,220 + LRK54_RS11465 0.84 -0.2 2,544,221 - LRK54_RS11465 0.84 +1.4 2,544,221 - LRK54_RS11465 0.84 -0.2 2,544,221 - LRK54_RS11465 0.84 +1.8 2,544,221 - LRK54_RS11465 0.84 +0.3 2,544,221 - LRK54_RS11465 0.84 -1.7 2,544,290 - +1.1 2,544,290 - -2.9 2,544,290 - -0.8 2,544,437 + -2.7 2,544,437 + +0.8 2,544,438 - +0.2 2,544,438 - -3.1 2,544,438 - +2.0 2,544,438 - +3.4 2,544,438 - +0.4 2,544,438 - -1.6 2,544,473 + +0.8 2,544,473 + +0.9 2,544,473 + -1.3 2,544,474 - +3.5 2,544,474 - +0.8 2,544,474 - -2.3 2,544,474 - -1.6 2,544,474 - +1.7 2,544,474 - +0.1 2,544,474 - +0.3 2,544,495 - LRK54_RS11470 0.10 +0.8 2,544,713 + LRK54_RS11470 0.40 -0.8 2,544,713 + LRK54_RS11470 0.40 +0.4 2,544,713 + LRK54_RS11470 0.40 -1.6 2,544,713 + LRK54_RS11470 0.40 +3.9 2,544,714 - LRK54_RS11470 0.40 +2.4 2,544,714 - LRK54_RS11470 0.40 +0.8 2,544,714 - LRK54_RS11470 0.40 -2.3 2,544,714 - LRK54_RS11470 0.40 -3.0 2,544,714 - LRK54_RS11470 0.40 +0.4 2,544,750 - LRK54_RS11470 0.45 -1.9 2,544,751 - LRK54_RS11470 0.45 +0.4 2,544,933 - LRK54_RS11470 0.70 -0.8 2,544,974 + LRK54_RS11470 0.76 -1.7 2,544,974 + LRK54_RS11470 0.76 -2.0 2,544,974 + LRK54_RS11470 0.76 -0.9 2,544,974 + LRK54_RS11470 0.76 +1.6 2,544,975 - LRK54_RS11470 0.76 -0.7 2,544,975 - LRK54_RS11470 0.76 +0.4 2,545,146 + -1.3 2,545,146 + -0.1 2,545,146 + -1.5 2,545,146 + +0.1 2,545,146 + -2.3 2,545,146 + -0.2 2,545,146 + +1.9 2,545,146 + -0.8 2,545,146 + +1.5 2,545,147 - -0.2 2,545,147 - -0.4 2,545,147 - -2.7 2,545,147 - -1.2 2,545,147 - -1.8 2,545,147 - -1.4 2,545,147 - +0.4 2,545,147 - -1.1 2,545,147 - +0.8 2,545,147 - -2.8 2,545,192 + +0.5 2,545,192 + -1.1 2,545,193 - +0.9 2,545,315 + +1.6 2,545,316 - -2.7 2,545,316 - -2.5 2,545,430 - LRK54_RS11475 0.17 -0.2 2,545,430 - LRK54_RS11475 0.17 -2.4 2,545,485 - LRK54_RS11475 0.20 +2.4 2,545,485 - LRK54_RS11475 0.20 +0.1 2,545,543 + LRK54_RS11475 0.23 +0.4 2,545,543 + LRK54_RS11475 0.23 +1.6 2,545,543 + LRK54_RS11475 0.23 +2.2 2,545,543 + LRK54_RS11475 0.23 -0.6 2,545,544 - LRK54_RS11475 0.24 +0.4 2,545,544 - LRK54_RS11475 0.24 +0.4 2,545,544 - LRK54_RS11475 0.24 -2.8 2,545,687 + LRK54_RS11475 0.32 -1.4 2,545,687 + LRK54_RS11475 0.32 +2.1 2,545,687 + LRK54_RS11475 0.32 -1.4 2,545,687 + LRK54_RS11475 0.32 -0.6 2,545,687 + LRK54_RS11475 0.32 +0.4 2,545,687 + LRK54_RS11475 0.32 +1.8 2,545,687 + LRK54_RS11475 0.32 +0.2 2,545,687 + LRK54_RS11475 0.32 -0.8 2,545,687 + LRK54_RS11475 0.32 -0.2 2,545,687 + LRK54_RS11475 0.32 +0.8 2,545,687 + LRK54_RS11475 0.32 +0.1 2,545,687 + LRK54_RS11475 0.32 +0.3 2,545,687 + LRK54_RS11475 0.32 +0.1 2,545,687 + LRK54_RS11475 0.32 +2.8 2,545,687 + LRK54_RS11475 0.32 -2.3 2,545,687 + LRK54_RS11475 0.32 -1.4 2,545,687 + LRK54_RS11475 0.32 -0.4 2,545,688 - LRK54_RS11475 0.32 +1.5 2,545,688 - LRK54_RS11475 0.32 +1.1 2,545,688 - LRK54_RS11475 0.32 -0.9 2,545,688 - LRK54_RS11475 0.32 +0.1 2,545,688 - LRK54_RS11475 0.32 -0.2 2,545,688 - LRK54_RS11475 0.32 -0.2 2,545,688 - LRK54_RS11475 0.32 +2.7 2,545,688 - LRK54_RS11475 0.32 -1.9 2,545,688 - LRK54_RS11475 0.32 +0.2 2,545,688 - LRK54_RS11475 0.32 -0.6 2,545,688 - LRK54_RS11475 0.32 -1.4 2,545,688 - LRK54_RS11475 0.32 -0.8 2,545,688 - LRK54_RS11475 0.32 +2.6 2,545,688 - LRK54_RS11475 0.32 -0.5 2,545,688 - LRK54_RS11475 0.32 -2.2 2,545,688 - LRK54_RS11475 0.32 -1.1 2,545,762 + LRK54_RS11475 0.36 -0.2 2,545,763 - LRK54_RS11475 0.37 -1.4 2,546,161 + LRK54_RS11475 0.60 -0.9 2,546,161 + LRK54_RS11475 0.60 -2.7 2,546,161 + LRK54_RS11475 0.60 -2.6 2,546,161 + LRK54_RS11475 0.60 +0.1 2,546,161 + LRK54_RS11475 0.60 +2.6 2,546,161 + LRK54_RS11475 0.60 -2.7 2,546,161 + LRK54_RS11475 0.60 -2.7 2,546,162 - LRK54_RS11475 0.60 -2.5 2,546,162 - LRK54_RS11475 0.60 +0.8 2,546,177 - LRK54_RS11475 0.61 +0.9 2,546,177 - LRK54_RS11475 0.61 -0.6 2,546,177 - LRK54_RS11475 0.61 +1.1 2,546,182 + LRK54_RS11475 0.61 +1.0 2,546,182 + LRK54_RS11475 0.61 +0.4 2,546,183 - LRK54_RS11475 0.62 -3.0 2,546,183 - LRK54_RS11475 0.62 -1.8 2,546,183 - LRK54_RS11475 0.62 +2.2 2,546,183 - LRK54_RS11475 0.62 +0.7 2,546,183 - LRK54_RS11475 0.62 -1.6 2,546,200 + LRK54_RS11475 0.63 -1.7 2,546,377 + LRK54_RS11475 0.73 +1.5 2,546,377 + LRK54_RS11475 0.73 +0.9 2,546,377 + LRK54_RS11475 0.73 -1.6 2,546,377 + LRK54_RS11475 0.73 -0.9 2,546,377 + LRK54_RS11475 0.73 -1.2 2,546,378 - LRK54_RS11475 0.73 -1.2 2,546,378 - LRK54_RS11475 0.73 +1.4 2,546,378 - LRK54_RS11475 0.73 -2.8 2,546,378 - LRK54_RS11475 0.73 -1.1 2,546,536 + LRK54_RS11475 0.83 -3.3 2,546,537 - LRK54_RS11475 0.83 -0.3 2,546,537 - LRK54_RS11475 0.83 -2.4 2,546,537 - LRK54_RS11475 0.83 -2.3 2,546,537 - LRK54_RS11475 0.83 +0.7 2,546,537 - LRK54_RS11475 0.83 -0.4 2,546,587 + LRK54_RS11475 0.86 -0.2 2,546,709 + +2.7 2,546,709 + +0.9 2,546,709 + +0.4 2,546,709 + -0.3 2,546,709 + -2.5 2,546,710 - -1.1 2,546,710 - +1.8 2,546,710 - -0.8 2,546,710 - -3.1 2,546,710 - -0.2 2,546,710 - +3.4 2,546,710 - +0.2 2,546,710 - -0.4 2,546,710 - +1.2 2,546,790 + -0.4 2,546,790 + +0.6 2,546,790 + -1.1 2,546,790 + -0.6 2,546,790 + +3.2 2,546,791 - -0.9 2,546,791 - -1.8 2,546,791 - -1.7 2,546,791 - -1.1 2,546,791 - +0.8 2,546,791 - -1.4 2,546,791 - -1.9 2,546,791 - -0.5 2,546,791 - -1.5 2,546,791 - +0.3 2,547,044 + LRK54_RS11480 0.19 -1.3 2,547,044 + LRK54_RS11480 0.19 -1.1 2,547,045 - LRK54_RS11480 0.19 +0.3 2,547,045 - LRK54_RS11480 0.19 +2.4 2,547,045 - LRK54_RS11480 0.19 -2.1 2,547,045 - LRK54_RS11480 0.19 -0.3 2,547,045 - LRK54_RS11480 0.19 -1.8 2,547,114 - LRK54_RS11480 0.25 -2.6 2,547,134 + LRK54_RS11480 0.26 +0.1 2,547,135 - LRK54_RS11480 0.26 +1.2 2,547,135 - LRK54_RS11480 0.26 -1.1 2,547,135 - LRK54_RS11480 0.26 +1.0 2,547,224 + LRK54_RS11480 0.34 -0.6 2,547,224 + LRK54_RS11480 0.34 -0.6 2,547,225 - LRK54_RS11480 0.34 +0.8 2,547,611 + LRK54_RS11480 0.66 +1.5 2,547,612 - LRK54_RS11480 0.66 -2.3 2,547,612 - LRK54_RS11480 0.66 -0.4 2,547,707 + LRK54_RS11480 0.74 +0.7 2,547,707 + LRK54_RS11480 0.74 -1.4 2,547,707 + LRK54_RS11480 0.74 +1.2 2,547,707 + LRK54_RS11480 0.74 -0.7 2,547,707 + LRK54_RS11480 0.74 -0.3 2,547,707 + LRK54_RS11480 0.74 +1.5 2,547,708 - LRK54_RS11480 0.74 -1.9 2,547,708 - LRK54_RS11480 0.74 +1.6 2,547,708 - LRK54_RS11480 0.74 -0.7 2,547,708 - LRK54_RS11480 0.74 +1.8 2,547,708 - LRK54_RS11480 0.74 +2.9 2,547,708 - LRK54_RS11480 0.74 +0.1 2,547,708 - LRK54_RS11480 0.74 -1.9 2,547,737 + LRK54_RS11480 0.76 +2.9 2,547,737 + LRK54_RS11480 0.76 +0.1 2,547,738 - LRK54_RS11480 0.76 +2.9 2,547,738 - LRK54_RS11480 0.76 +0.7 2,547,771 - LRK54_RS11480 0.79 -1.6
Or see this region's nucleotide sequence