Experiment: R2A with bismuth(III) chloride 0.03125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02205 and LRK54_RS02210 overlap by 4 nucleotides LRK54_RS02210 and LRK54_RS02215 overlap by 4 nucleotides LRK54_RS02215 and LRK54_RS02220 are separated by 176 nucleotides
LRK54_RS02205: LRK54_RS02205 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 480,932 to 481,984
_RS02205
LRK54_RS02210: LRK54_RS02210 - type IV pilus modification protein PilV, at 481,981 to 482,508
_RS02210
LRK54_RS02215: LRK54_RS02215 - GspH/FimT family pseudopilin, at 482,505 to 483,137
_RS02215
LRK54_RS02220: LRK54_RS02220 - GGDEF domain-containing protein, at 483,314 to 485,170
_RS02220
Position (kb) 482
483
484 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 481.630 kb on + strand, within LRK54_RS02205 at 481.821 kb on + strand, within LRK54_RS02205 at 481.821 kb on + strand, within LRK54_RS02205 at 481.821 kb on + strand, within LRK54_RS02205 at 481.822 kb on - strand, within LRK54_RS02205 at 481.822 kb on - strand, within LRK54_RS02205 at 481.822 kb on - strand, within LRK54_RS02205 at 481.822 kb on - strand, within LRK54_RS02205 at 481.822 kb on - strand, within LRK54_RS02205 at 481.942 kb on + strand at 481.942 kb on + strand at 481.942 kb on + strand at 481.942 kb on + strand at 481.942 kb on + strand at 481.942 kb on + strand at 481.942 kb on + strand at 481.943 kb on - strand at 481.943 kb on - strand at 481.943 kb on - strand at 481.943 kb on - strand at 481.943 kb on - strand at 481.966 kb on + strand at 481.966 kb on + strand at 481.966 kb on + strand at 481.966 kb on + strand at 481.967 kb on - strand at 481.967 kb on - strand at 481.967 kb on - strand at 481.967 kb on - strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.984 kb on + strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 481.985 kb on - strand at 482.159 kb on + strand, within LRK54_RS02210 at 482.160 kb on - strand, within LRK54_RS02210 at 482.160 kb on - strand, within LRK54_RS02210 at 482.160 kb on - strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.270 kb on + strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.271 kb on - strand, within LRK54_RS02210 at 482.324 kb on + strand, within LRK54_RS02210 at 482.324 kb on + strand, within LRK54_RS02210 at 482.324 kb on + strand, within LRK54_RS02210 at 482.324 kb on + strand, within LRK54_RS02210 at 482.324 kb on + strand, within LRK54_RS02210 at 482.324 kb on + strand, within LRK54_RS02210 at 482.325 kb on - strand, within LRK54_RS02210 at 482.325 kb on - strand, within LRK54_RS02210 at 482.325 kb on - strand, within LRK54_RS02210 at 482.325 kb on - strand, within LRK54_RS02210 at 482.325 kb on - strand, within LRK54_RS02210 at 482.436 kb on + strand, within LRK54_RS02210 at 482.437 kb on - strand, within LRK54_RS02210 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.629 kb on + strand, within LRK54_RS02215 at 482.630 kb on - strand, within LRK54_RS02215 at 482.630 kb on - strand, within LRK54_RS02215 at 482.630 kb on - strand, within LRK54_RS02215 at 482.630 kb on - strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.773 kb on + strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 482.774 kb on - strand, within LRK54_RS02215 at 483.169 kb on + strand at 483.169 kb on + strand at 483.169 kb on + strand at 483.169 kb on + strand at 483.169 kb on + strand at 483.170 kb on - strand at 483.170 kb on - strand at 483.170 kb on - strand at 483.170 kb on - strand at 483.170 kb on - strand at 483.170 kb on - strand at 483.170 kb on - strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.360 kb on + strand at 483.361 kb on - strand at 483.361 kb on - strand at 483.361 kb on - strand at 483.361 kb on - strand at 483.361 kb on - strand at 483.361 kb on - strand at 483.361 kb on - strand at 483.361 kb on - strand at 483.763 kb on + strand, within LRK54_RS02220 at 483.763 kb on + strand, within LRK54_RS02220 at 483.764 kb on - strand, within LRK54_RS02220 at 483.764 kb on - strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.005 kb on + strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.006 kb on - strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220 at 484.137 kb on + strand, within LRK54_RS02220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with bismuth(III) chloride 0.03125 mM remove 481,630 + LRK54_RS02205 0.66 +1.3 481,821 + LRK54_RS02205 0.84 -1.9 481,821 + LRK54_RS02205 0.84 +0.8 481,821 + LRK54_RS02205 0.84 -0.3 481,822 - LRK54_RS02205 0.85 +0.8 481,822 - LRK54_RS02205 0.85 +3.1 481,822 - LRK54_RS02205 0.85 +0.5 481,822 - LRK54_RS02205 0.85 +1.2 481,822 - LRK54_RS02205 0.85 -0.5 481,942 + +2.1 481,942 + -1.4 481,942 + +0.4 481,942 + +0.9 481,942 + +1.2 481,942 + -0.6 481,942 + +1.9 481,943 - -2.8 481,943 - -0.9 481,943 - -0.1 481,943 - -0.5 481,943 - -0.8 481,966 + -0.8 481,966 + -0.5 481,966 + +1.3 481,966 + +2.6 481,967 - +2.7 481,967 - -0.5 481,967 - -0.1 481,967 - +2.3 481,984 + +0.6 481,984 + +0.2 481,984 + +0.2 481,984 + -0.3 481,984 + -0.9 481,984 + +0.8 481,984 + -0.1 481,984 + -0.9 481,984 + +0.4 481,984 + -2.3 481,984 + -1.1 481,984 + -1.1 481,984 + -1.2 481,984 + +1.2 481,984 + -0.9 481,984 + -1.6 481,984 + -1.3 481,984 + -1.2 481,984 + +0.9 481,984 + -0.1 481,984 + -0.5 481,984 + -0.9 481,984 + -3.7 481,984 + -0.3 481,984 + +1.4 481,984 + +2.1 481,984 + -0.1 481,984 + -0.6 481,984 + +1.4 481,984 + -2.0 481,984 + -1.5 481,984 + -0.0 481,984 + -1.6 481,984 + -1.4 481,984 + -1.8 481,984 + -0.6 481,984 + -1.1 481,984 + +1.5 481,984 + +0.5 481,984 + +0.3 481,985 - +1.3 481,985 - -1.6 481,985 - +0.3 481,985 - +1.8 481,985 - +0.9 481,985 - +1.7 481,985 - +0.1 481,985 - +2.5 481,985 - -1.9 481,985 - -0.9 481,985 - +2.9 481,985 - -0.6 481,985 - -1.3 481,985 - +2.2 481,985 - -0.7 481,985 - +0.8 481,985 - -1.3 481,985 - +1.9 481,985 - +2.4 481,985 - +0.7 481,985 - -2.6 481,985 - +2.3 481,985 - -1.7 481,985 - +4.3 481,985 - +0.2 481,985 - +1.5 481,985 - +3.0 481,985 - -1.6 481,985 - -2.1 481,985 - +2.1 481,985 - -2.5 481,985 - -1.7 481,985 - +0.9 482,159 + LRK54_RS02210 0.34 +0.2 482,160 - LRK54_RS02210 0.34 +0.4 482,160 - LRK54_RS02210 0.34 -2.0 482,160 - LRK54_RS02210 0.34 +0.0 482,270 + LRK54_RS02210 0.55 +1.1 482,270 + LRK54_RS02210 0.55 +1.9 482,270 + LRK54_RS02210 0.55 +1.2 482,270 + LRK54_RS02210 0.55 -0.3 482,270 + LRK54_RS02210 0.55 -0.1 482,270 + LRK54_RS02210 0.55 +2.8 482,270 + LRK54_RS02210 0.55 +1.2 482,270 + LRK54_RS02210 0.55 +2.8 482,270 + LRK54_RS02210 0.55 -1.9 482,270 + LRK54_RS02210 0.55 +0.4 482,270 + LRK54_RS02210 0.55 +1.4 482,270 + LRK54_RS02210 0.55 +1.1 482,270 + LRK54_RS02210 0.55 -0.1 482,271 - LRK54_RS02210 0.55 +0.4 482,271 - LRK54_RS02210 0.55 +0.5 482,271 - LRK54_RS02210 0.55 +0.9 482,271 - LRK54_RS02210 0.55 +2.6 482,271 - LRK54_RS02210 0.55 +1.8 482,271 - LRK54_RS02210 0.55 -1.7 482,271 - LRK54_RS02210 0.55 +2.0 482,271 - LRK54_RS02210 0.55 +0.7 482,324 + LRK54_RS02210 0.65 -0.9 482,324 + LRK54_RS02210 0.65 +0.1 482,324 + LRK54_RS02210 0.65 +0.5 482,324 + LRK54_RS02210 0.65 -0.6 482,324 + LRK54_RS02210 0.65 +1.6 482,324 + LRK54_RS02210 0.65 +0.9 482,325 - LRK54_RS02210 0.65 -1.2 482,325 - LRK54_RS02210 0.65 +2.0 482,325 - LRK54_RS02210 0.65 +1.5 482,325 - LRK54_RS02210 0.65 +2.2 482,325 - LRK54_RS02210 0.65 +1.3 482,436 + LRK54_RS02210 0.86 -0.5 482,437 - LRK54_RS02210 0.86 +1.9 482,629 + LRK54_RS02215 0.20 -2.4 482,629 + LRK54_RS02215 0.20 +1.1 482,629 + LRK54_RS02215 0.20 -1.4 482,629 + LRK54_RS02215 0.20 +3.7 482,629 + LRK54_RS02215 0.20 +1.8 482,629 + LRK54_RS02215 0.20 -0.6 482,629 + LRK54_RS02215 0.20 +2.2 482,629 + LRK54_RS02215 0.20 +3.5 482,629 + LRK54_RS02215 0.20 +2.5 482,630 - LRK54_RS02215 0.20 -0.6 482,630 - LRK54_RS02215 0.20 -1.7 482,630 - LRK54_RS02215 0.20 +1.3 482,630 - LRK54_RS02215 0.20 +2.7 482,773 + LRK54_RS02215 0.42 -0.0 482,773 + LRK54_RS02215 0.42 +2.3 482,773 + LRK54_RS02215 0.42 +0.2 482,773 + LRK54_RS02215 0.42 +2.5 482,773 + LRK54_RS02215 0.42 -1.6 482,773 + LRK54_RS02215 0.42 -0.5 482,773 + LRK54_RS02215 0.42 +2.2 482,773 + LRK54_RS02215 0.42 -0.8 482,773 + LRK54_RS02215 0.42 -0.9 482,773 + LRK54_RS02215 0.42 +2.5 482,773 + LRK54_RS02215 0.42 -2.7 482,773 + LRK54_RS02215 0.42 +0.9 482,773 + LRK54_RS02215 0.42 -0.3 482,773 + LRK54_RS02215 0.42 +1.2 482,773 + LRK54_RS02215 0.42 +3.0 482,774 - LRK54_RS02215 0.42 +2.6 482,774 - LRK54_RS02215 0.42 +2.3 482,774 - LRK54_RS02215 0.42 +1.3 482,774 - LRK54_RS02215 0.42 +5.3 482,774 - LRK54_RS02215 0.42 +0.3 482,774 - LRK54_RS02215 0.42 +2.9 482,774 - LRK54_RS02215 0.42 +0.2 482,774 - LRK54_RS02215 0.42 +1.8 482,774 - LRK54_RS02215 0.42 -0.6 482,774 - LRK54_RS02215 0.42 -2.4 482,774 - LRK54_RS02215 0.42 +2.8 482,774 - LRK54_RS02215 0.42 +0.9 482,774 - LRK54_RS02215 0.42 -0.6 482,774 - LRK54_RS02215 0.42 -0.8 482,774 - LRK54_RS02215 0.42 -1.5 482,774 - LRK54_RS02215 0.42 -0.6 482,774 - LRK54_RS02215 0.42 -1.6 482,774 - LRK54_RS02215 0.42 -0.3 482,774 - LRK54_RS02215 0.42 +3.7 482,774 - LRK54_RS02215 0.42 +0.5 482,774 - LRK54_RS02215 0.42 +0.5 483,169 + +0.5 483,169 + -0.6 483,169 + +2.0 483,169 + +1.0 483,169 + +2.1 483,170 - +0.2 483,170 - +1.6 483,170 - -0.9 483,170 - +1.7 483,170 - -0.3 483,170 - -1.5 483,170 - +1.0 483,360 + -0.0 483,360 + +0.8 483,360 + -0.8 483,360 + -0.5 483,360 + -3.3 483,360 + -0.1 483,360 + -0.3 483,360 + -2.1 483,360 + -2.7 483,360 + -2.4 483,360 + +0.6 483,361 - -0.6 483,361 - -0.7 483,361 - +0.5 483,361 - -0.8 483,361 - +0.2 483,361 - +3.8 483,361 - -1.0 483,361 - +4.1 483,763 + LRK54_RS02220 0.24 -2.4 483,763 + LRK54_RS02220 0.24 +0.5 483,764 - LRK54_RS02220 0.24 +1.5 483,764 - LRK54_RS02220 0.24 -1.2 484,005 + LRK54_RS02220 0.37 +1.5 484,005 + LRK54_RS02220 0.37 -0.6 484,005 + LRK54_RS02220 0.37 -0.0 484,005 + LRK54_RS02220 0.37 +0.2 484,005 + LRK54_RS02220 0.37 -2.2 484,005 + LRK54_RS02220 0.37 -1.5 484,005 + LRK54_RS02220 0.37 -0.5 484,005 + LRK54_RS02220 0.37 -1.6 484,006 - LRK54_RS02220 0.37 +2.7 484,006 - LRK54_RS02220 0.37 -2.3 484,006 - LRK54_RS02220 0.37 -1.2 484,006 - LRK54_RS02220 0.37 +0.6 484,006 - LRK54_RS02220 0.37 +0.5 484,006 - LRK54_RS02220 0.37 -0.2 484,006 - LRK54_RS02220 0.37 -0.5 484,006 - LRK54_RS02220 0.37 -1.6 484,137 + LRK54_RS02220 0.44 -0.0 484,137 + LRK54_RS02220 0.44 +1.9 484,137 + LRK54_RS02220 0.44 +0.7 484,137 + LRK54_RS02220 0.44 -2.0 484,137 + LRK54_RS02220 0.44 -0.2 484,137 + LRK54_RS02220 0.44 +1.5 484,137 + LRK54_RS02220 0.44 -1.9 484,137 + LRK54_RS02220 0.44 +1.7 484,137 + LRK54_RS02220 0.44 -1.9 484,137 + LRK54_RS02220 0.44 +2.5 484,137 + LRK54_RS02220 0.44 -0.0 484,137 + LRK54_RS02220 0.44 +0.5 484,137 + LRK54_RS02220 0.44 -4.2 484,137 + LRK54_RS02220 0.44 +2.6 484,137 + LRK54_RS02220 0.44 -0.9 484,137 + LRK54_RS02220 0.44 +0.9 484,137 + LRK54_RS02220 0.44 -1.9 484,137 + LRK54_RS02220 0.44 +2.8 484,137 + LRK54_RS02220 0.44 -1.4 484,137 + LRK54_RS02220 0.44 -0.5
Or see this region's nucleotide sequence