Experiment: Maltitol 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18660 and CA265_RS18665 are separated by 187 nucleotides CA265_RS18665 and CA265_RS18670 are separated by 94 nucleotides CA265_RS18670 and CA265_RS18675 are separated by 208 nucleotides CA265_RS18675 and CA265_RS18680 are separated by 173 nucleotides
CA265_RS18660: CA265_RS18660 - SAM-dependent methyltransferase, at 4,641,551 to 4,642,252
_RS18660
CA265_RS18665: CA265_RS18665 - hypothetical protein, at 4,642,440 to 4,642,619
_RS18665
CA265_RS18670: CA265_RS18670 - damage-inducible protein DinB, at 4,642,714 to 4,643,181
_RS18670
CA265_RS18675: CA265_RS18675 - hypothetical protein, at 4,643,390 to 4,643,734
_RS18675
CA265_RS18680: CA265_RS18680 - hypothetical protein, at 4,643,908 to 4,644,786
_RS18680
Position (kb)
4642
4643
4644 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4641.754 kb on + strand, within CA265_RS18660 at 4641.769 kb on - strand, within CA265_RS18660 at 4641.814 kb on - strand, within CA265_RS18660 at 4641.878 kb on - strand, within CA265_RS18660 at 4641.878 kb on - strand, within CA265_RS18660 at 4641.898 kb on - strand, within CA265_RS18660 at 4641.904 kb on - strand, within CA265_RS18660 at 4641.904 kb on - strand, within CA265_RS18660 at 4641.904 kb on - strand, within CA265_RS18660 at 4641.982 kb on - strand, within CA265_RS18660 at 4641.992 kb on - strand, within CA265_RS18660 at 4642.127 kb on - strand, within CA265_RS18660 at 4642.174 kb on + strand, within CA265_RS18660 at 4642.212 kb on + strand at 4642.231 kb on + strand at 4642.231 kb on + strand at 4642.236 kb on + strand at 4642.247 kb on + strand at 4642.312 kb on - strand at 4642.313 kb on + strand at 4642.314 kb on - strand at 4642.315 kb on + strand at 4642.315 kb on + strand at 4642.389 kb on + strand at 4642.399 kb on + strand at 4642.399 kb on + strand at 4642.400 kb on - strand at 4642.423 kb on + strand at 4642.543 kb on + strand, within CA265_RS18665 at 4642.590 kb on - strand, within CA265_RS18665 at 4642.602 kb on + strand at 4642.633 kb on - strand at 4642.704 kb on - strand at 4642.731 kb on + strand at 4642.734 kb on - strand at 4642.740 kb on + strand at 4642.742 kb on + strand at 4642.742 kb on + strand at 4642.742 kb on + strand at 4642.765 kb on + strand, within CA265_RS18670 at 4642.765 kb on + strand, within CA265_RS18670 at 4642.772 kb on - strand, within CA265_RS18670 at 4642.776 kb on + strand, within CA265_RS18670 at 4642.808 kb on + strand, within CA265_RS18670 at 4642.903 kb on - strand, within CA265_RS18670 at 4642.911 kb on + strand, within CA265_RS18670 at 4642.912 kb on - strand, within CA265_RS18670 at 4642.925 kb on + strand, within CA265_RS18670 at 4642.925 kb on + strand, within CA265_RS18670 at 4642.926 kb on - strand, within CA265_RS18670 at 4643.030 kb on + strand, within CA265_RS18670 at 4643.030 kb on + strand, within CA265_RS18670 at 4643.031 kb on - strand, within CA265_RS18670 at 4643.049 kb on + strand, within CA265_RS18670 at 4643.087 kb on - strand, within CA265_RS18670 at 4643.257 kb on - strand at 4643.288 kb on + strand at 4643.369 kb on + strand at 4643.369 kb on + strand at 4643.370 kb on - strand at 4643.391 kb on + strand at 4643.657 kb on + strand, within CA265_RS18675 at 4643.692 kb on + strand, within CA265_RS18675 at 4643.693 kb on - strand, within CA265_RS18675 at 4643.708 kb on - strand at 4643.708 kb on - strand at 4643.712 kb on + strand at 4643.734 kb on + strand at 4643.765 kb on - strand at 4643.950 kb on - strand at 4644.145 kb on + strand at 4644.145 kb on + strand, within CA265_RS18680 at 4644.146 kb on - strand, within CA265_RS18680 at 4644.146 kb on - strand, within CA265_RS18680
Per-strain Table
Position Strand Gene LocusTag Fraction Maltitol 20mM (C) remove 4,641,754 + CA265_RS18660 0.29 +1.9 4,641,769 - CA265_RS18660 0.31 +0.5 4,641,814 - CA265_RS18660 0.37 -1.4 4,641,878 - CA265_RS18660 0.47 +1.1 4,641,878 - CA265_RS18660 0.47 -0.9 4,641,898 - CA265_RS18660 0.49 -0.4 4,641,904 - CA265_RS18660 0.50 -0.2 4,641,904 - CA265_RS18660 0.50 -2.0 4,641,904 - CA265_RS18660 0.50 -0.1 4,641,982 - CA265_RS18660 0.61 +0.2 4,641,992 - CA265_RS18660 0.63 -0.0 4,642,127 - CA265_RS18660 0.82 +0.9 4,642,174 + CA265_RS18660 0.89 +1.6 4,642,212 + -0.4 4,642,231 + -2.3 4,642,231 + +0.7 4,642,236 + -0.5 4,642,247 + +2.6 4,642,312 - +1.3 4,642,313 + +0.5 4,642,314 - -1.9 4,642,315 + +1.0 4,642,315 + -0.8 4,642,389 + +0.1 4,642,399 + -0.0 4,642,399 + -1.1 4,642,400 - -0.3 4,642,423 + +0.1 4,642,543 + CA265_RS18665 0.57 -0.3 4,642,590 - CA265_RS18665 0.83 -1.6 4,642,602 + +0.5 4,642,633 - -1.1 4,642,704 - -1.6 4,642,731 + -1.1 4,642,734 - -0.5 4,642,740 + +0.3 4,642,742 + -0.7 4,642,742 + +1.6 4,642,742 + +1.7 4,642,765 + CA265_RS18670 0.11 +0.1 4,642,765 + CA265_RS18670 0.11 +1.1 4,642,772 - CA265_RS18670 0.12 -0.4 4,642,776 + CA265_RS18670 0.13 -1.6 4,642,808 + CA265_RS18670 0.20 -0.8 4,642,903 - CA265_RS18670 0.40 +0.2 4,642,911 + CA265_RS18670 0.42 -0.7 4,642,912 - CA265_RS18670 0.42 -0.2 4,642,925 + CA265_RS18670 0.45 -1.3 4,642,925 + CA265_RS18670 0.45 +0.3 4,642,926 - CA265_RS18670 0.45 -1.0 4,643,030 + CA265_RS18670 0.68 -1.5 4,643,030 + CA265_RS18670 0.68 -0.9 4,643,031 - CA265_RS18670 0.68 -0.1 4,643,049 + CA265_RS18670 0.72 -0.6 4,643,087 - CA265_RS18670 0.80 +0.0 4,643,257 - -3.7 4,643,288 + -0.1 4,643,369 + +0.5 4,643,369 + -0.0 4,643,370 - -1.9 4,643,391 + +0.0 4,643,657 + CA265_RS18675 0.77 -0.7 4,643,692 + CA265_RS18675 0.88 +0.6 4,643,693 - CA265_RS18675 0.88 +0.1 4,643,708 - +2.0 4,643,708 - +0.3 4,643,712 + +0.2 4,643,734 + -1.2 4,643,765 - -1.8 4,643,950 - -1.5 4,644,145 + -1.4 4,644,145 + CA265_RS18680 0.27 -2.3 4,644,146 - CA265_RS18680 0.27 -0.9 4,644,146 - CA265_RS18680 0.27 -1.5
Or see this region's nucleotide sequence