Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02558 and CCNA_02559 overlap by 4 nucleotides CCNA_02559 and CCNA_02560 are separated by 123 nucleotides CCNA_02560 and CCNA_02561 overlap by 4 nucleotides
CCNA_02558: CCNA_02558 - enantioselective peptide amidase, at 2,704,653 to 2,706,239
_02558
CCNA_02559: CCNA_02559 - NTF2 superfamily protein, at 2,706,236 to 2,706,730
_02559
CCNA_02560: CCNA_02560 - stalk cross-band protein StpC, at 2,706,854 to 2,707,492
_02560
CCNA_02561: CCNA_02561 - stalk cross-band protein StpB, at 2,707,489 to 2,708,913
_02561
Position (kb)
2706
2707
2708 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2705.856 kb on - strand, within CCNA_02558 at 2705.867 kb on - strand, within CCNA_02558 at 2705.874 kb on + strand at 2705.875 kb on - strand, within CCNA_02558 at 2705.875 kb on - strand, within CCNA_02558 at 2705.882 kb on - strand, within CCNA_02558 at 2705.902 kb on - strand, within CCNA_02558 at 2705.902 kb on - strand, within CCNA_02558 at 2705.902 kb on - strand, within CCNA_02558 at 2705.906 kb on + strand, within CCNA_02558 at 2705.909 kb on - strand, within CCNA_02558 at 2705.919 kb on + strand, within CCNA_02558 at 2705.937 kb on - strand, within CCNA_02558 at 2705.937 kb on - strand, within CCNA_02558 at 2705.937 kb on - strand, within CCNA_02558 at 2705.999 kb on + strand, within CCNA_02558 at 2705.999 kb on + strand, within CCNA_02558 at 2706.007 kb on + strand, within CCNA_02558 at 2706.007 kb on + strand, within CCNA_02558 at 2706.078 kb on + strand, within CCNA_02558 at 2706.103 kb on + strand at 2706.103 kb on + strand at 2706.111 kb on - strand at 2706.111 kb on - strand at 2706.143 kb on - strand at 2706.148 kb on - strand at 2706.178 kb on - strand at 2706.192 kb on + strand at 2706.215 kb on - strand at 2706.223 kb on - strand at 2706.296 kb on + strand, within CCNA_02559 at 2706.296 kb on + strand, within CCNA_02559 at 2706.297 kb on + strand, within CCNA_02559 at 2706.297 kb on + strand, within CCNA_02559 at 2706.297 kb on + strand, within CCNA_02559 at 2706.304 kb on - strand, within CCNA_02559 at 2706.305 kb on - strand, within CCNA_02559 at 2706.305 kb on - strand, within CCNA_02559 at 2706.329 kb on + strand, within CCNA_02559 at 2706.337 kb on - strand, within CCNA_02559 at 2706.337 kb on - strand, within CCNA_02559 at 2706.345 kb on + strand, within CCNA_02559 at 2706.365 kb on - strand, within CCNA_02559 at 2706.406 kb on - strand, within CCNA_02559 at 2706.406 kb on - strand, within CCNA_02559 at 2706.441 kb on + strand, within CCNA_02559 at 2706.484 kb on - strand, within CCNA_02559 at 2706.484 kb on - strand, within CCNA_02559 at 2706.511 kb on - strand, within CCNA_02559 at 2706.511 kb on - strand, within CCNA_02559 at 2706.522 kb on - strand, within CCNA_02559 at 2706.562 kb on - strand, within CCNA_02559 at 2706.623 kb on + strand, within CCNA_02559 at 2706.623 kb on + strand, within CCNA_02559 at 2706.631 kb on - strand, within CCNA_02559 at 2706.631 kb on - strand, within CCNA_02559 at 2706.696 kb on + strand at 2706.698 kb on + strand at 2706.698 kb on + strand at 2706.706 kb on - strand at 2706.709 kb on - strand at 2706.711 kb on - strand at 2706.845 kb on + strand at 2706.853 kb on - strand at 2706.890 kb on + strand at 2706.925 kb on - strand, within CCNA_02560 at 2706.930 kb on - strand, within CCNA_02560 at 2706.962 kb on + strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.971 kb on - strand, within CCNA_02560 at 2707.041 kb on + strand, within CCNA_02560 at 2707.054 kb on - strand, within CCNA_02560 at 2707.070 kb on + strand, within CCNA_02560 at 2707.078 kb on - strand, within CCNA_02560 at 2707.078 kb on - strand, within CCNA_02560 at 2707.082 kb on + strand, within CCNA_02560 at 2707.103 kb on + strand, within CCNA_02560 at 2707.143 kb on + strand, within CCNA_02560 at 2707.151 kb on - strand, within CCNA_02560 at 2707.151 kb on - strand, within CCNA_02560 at 2707.171 kb on - strand, within CCNA_02560 at 2707.174 kb on + strand, within CCNA_02560 at 2707.174 kb on + strand, within CCNA_02560 at 2707.182 kb on - strand, within CCNA_02560 at 2707.184 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.192 kb on - strand, within CCNA_02560 at 2707.192 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.199 kb on + strand, within CCNA_02560 at 2707.199 kb on + strand, within CCNA_02560 at 2707.199 kb on + strand, within CCNA_02560 at 2707.240 kb on - strand, within CCNA_02560 at 2707.317 kb on + strand, within CCNA_02560 at 2707.318 kb on - strand, within CCNA_02560 at 2707.318 kb on - strand, within CCNA_02560 at 2707.318 kb on - strand, within CCNA_02560 at 2707.358 kb on + strand, within CCNA_02560 at 2707.361 kb on - strand, within CCNA_02560 at 2707.361 kb on - strand, within CCNA_02560 at 2707.361 kb on - strand, within CCNA_02560 at 2707.405 kb on + strand, within CCNA_02560 at 2707.407 kb on + strand, within CCNA_02560 at 2707.407 kb on + strand, within CCNA_02560 at 2707.408 kb on - strand, within CCNA_02560 at 2707.408 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.428 kb on + strand, within CCNA_02560 at 2707.430 kb on + strand at 2707.430 kb on + strand at 2707.436 kb on - strand at 2707.456 kb on + strand at 2707.480 kb on - strand at 2707.480 kb on - strand at 2707.482 kb on + strand at 2707.512 kb on - strand at 2707.512 kb on - strand at 2707.563 kb on + strand at 2707.601 kb on + strand at 2707.601 kb on + strand at 2707.609 kb on - strand at 2707.633 kb on + strand, within CCNA_02561 at 2707.651 kb on - strand, within CCNA_02561 at 2707.699 kb on - strand, within CCNA_02561 at 2707.703 kb on + strand, within CCNA_02561 at 2707.703 kb on + strand, within CCNA_02561 at 2707.711 kb on - strand, within CCNA_02561 at 2707.724 kb on + strand, within CCNA_02561 at 2707.724 kb on + strand, within CCNA_02561 at 2707.725 kb on + strand, within CCNA_02561 at 2707.747 kb on + strand, within CCNA_02561 at 2707.755 kb on - strand, within CCNA_02561 at 2707.755 kb on - strand, within CCNA_02561 at 2707.765 kb on - strand, within CCNA_02561 at 2707.777 kb on - strand, within CCNA_02561 at 2707.847 kb on + strand, within CCNA_02561 at 2707.868 kb on + strand, within CCNA_02561 at 2707.868 kb on + strand, within CCNA_02561 at 2707.876 kb on - strand, within CCNA_02561 at 2707.880 kb on + strand, within CCNA_02561 at 2707.888 kb on - strand, within CCNA_02561 at 2707.888 kb on - strand, within CCNA_02561 at 2707.896 kb on + strand, within CCNA_02561 at 2707.946 kb on - strand, within CCNA_02561 at 2708.020 kb on - strand, within CCNA_02561 at 2708.026 kb on - strand, within CCNA_02561 at 2708.039 kb on - strand, within CCNA_02561 at 2708.216 kb on + strand, within CCNA_02561 at 2708.251 kb on - strand, within CCNA_02561 at 2708.256 kb on - strand, within CCNA_02561 at 2708.256 kb on - strand, within CCNA_02561 at 2708.259 kb on + strand, within CCNA_02561 at 2708.277 kb on - strand, within CCNA_02561 at 2708.364 kb on - strand, within CCNA_02561 at 2708.366 kb on + strand, within CCNA_02561 at 2708.397 kb on - strand, within CCNA_02561 at 2708.406 kb on - strand, within CCNA_02561 at 2708.406 kb on - strand, within CCNA_02561 at 2708.406 kb on - strand, within CCNA_02561 at 2708.435 kb on + strand, within CCNA_02561 at 2708.435 kb on + strand, within CCNA_02561
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 2,705,856 - CCNA_02558 0.76 -1.3 2,705,867 - CCNA_02558 0.76 -0.6 2,705,874 + +1.8 2,705,875 - CCNA_02558 0.77 +1.0 2,705,875 - CCNA_02558 0.77 -1.6 2,705,882 - CCNA_02558 0.77 -1.2 2,705,902 - CCNA_02558 0.79 -0.4 2,705,902 - CCNA_02558 0.79 +3.8 2,705,902 - CCNA_02558 0.79 +0.8 2,705,906 + CCNA_02558 0.79 -0.4 2,705,909 - CCNA_02558 0.79 -1.8 2,705,919 + CCNA_02558 0.80 -0.4 2,705,937 - CCNA_02558 0.81 -0.8 2,705,937 - CCNA_02558 0.81 -2.1 2,705,937 - CCNA_02558 0.81 -1.2 2,705,999 + CCNA_02558 0.85 +3.4 2,705,999 + CCNA_02558 0.85 -0.5 2,706,007 + CCNA_02558 0.85 +2.3 2,706,007 + CCNA_02558 0.85 +1.9 2,706,078 + CCNA_02558 0.90 +0.7 2,706,103 + +1.2 2,706,103 + +0.4 2,706,111 - -1.4 2,706,111 - +0.8 2,706,143 - -1.0 2,706,148 - -0.0 2,706,178 - -0.6 2,706,192 + -0.7 2,706,215 - +0.3 2,706,223 - +0.2 2,706,296 + CCNA_02559 0.12 +0.0 2,706,296 + CCNA_02559 0.12 +0.2 2,706,297 + CCNA_02559 0.12 -1.0 2,706,297 + CCNA_02559 0.12 -0.7 2,706,297 + CCNA_02559 0.12 -0.1 2,706,304 - CCNA_02559 0.14 -1.4 2,706,305 - CCNA_02559 0.14 +0.3 2,706,305 - CCNA_02559 0.14 -1.2 2,706,329 + CCNA_02559 0.19 +0.3 2,706,337 - CCNA_02559 0.20 -2.1 2,706,337 - CCNA_02559 0.20 -0.7 2,706,345 + CCNA_02559 0.22 +2.0 2,706,365 - CCNA_02559 0.26 +2.2 2,706,406 - CCNA_02559 0.34 -1.1 2,706,406 - CCNA_02559 0.34 -0.6 2,706,441 + CCNA_02559 0.41 -0.8 2,706,484 - CCNA_02559 0.50 -1.1 2,706,484 - CCNA_02559 0.50 -0.4 2,706,511 - CCNA_02559 0.56 -0.0 2,706,511 - CCNA_02559 0.56 +1.2 2,706,522 - CCNA_02559 0.58 +0.4 2,706,562 - CCNA_02559 0.66 +1.4 2,706,623 + CCNA_02559 0.78 +2.8 2,706,623 + CCNA_02559 0.78 +0.4 2,706,631 - CCNA_02559 0.80 -0.2 2,706,631 - CCNA_02559 0.80 -0.2 2,706,696 + -2.7 2,706,698 + +0.3 2,706,698 + -1.2 2,706,706 - +1.6 2,706,709 - +2.4 2,706,711 - +0.9 2,706,845 + +1.6 2,706,853 - +2.7 2,706,890 + +0.0 2,706,925 - CCNA_02560 0.11 +1.7 2,706,930 - CCNA_02560 0.12 +2.0 2,706,962 + CCNA_02560 0.17 +0.8 2,706,970 - CCNA_02560 0.18 +2.2 2,706,970 - CCNA_02560 0.18 +2.7 2,706,970 - CCNA_02560 0.18 +5.1 2,706,970 - CCNA_02560 0.18 +2.6 2,706,970 - CCNA_02560 0.18 +2.3 2,706,971 - CCNA_02560 0.18 +2.8 2,707,041 + CCNA_02560 0.29 -0.0 2,707,054 - CCNA_02560 0.31 +2.6 2,707,070 + CCNA_02560 0.34 -1.0 2,707,078 - CCNA_02560 0.35 +2.5 2,707,078 - CCNA_02560 0.35 +2.3 2,707,082 + CCNA_02560 0.36 +0.8 2,707,103 + CCNA_02560 0.39 -1.0 2,707,143 + CCNA_02560 0.45 -1.7 2,707,151 - CCNA_02560 0.46 +1.5 2,707,151 - CCNA_02560 0.46 +3.4 2,707,171 - CCNA_02560 0.50 +3.0 2,707,174 + CCNA_02560 0.50 +1.0 2,707,174 + CCNA_02560 0.50 -1.1 2,707,182 - CCNA_02560 0.51 +2.4 2,707,184 + CCNA_02560 0.52 +1.0 2,707,189 + CCNA_02560 0.52 -1.2 2,707,189 + CCNA_02560 0.52 +1.4 2,707,189 + CCNA_02560 0.52 +0.3 2,707,189 + CCNA_02560 0.52 -0.4 2,707,192 - CCNA_02560 0.53 +2.9 2,707,192 - CCNA_02560 0.53 +2.6 2,707,197 - CCNA_02560 0.54 +0.9 2,707,197 - CCNA_02560 0.54 +2.9 2,707,197 - CCNA_02560 0.54 +3.1 2,707,197 - CCNA_02560 0.54 +2.1 2,707,197 - CCNA_02560 0.54 +3.4 2,707,199 + CCNA_02560 0.54 -1.2 2,707,199 + CCNA_02560 0.54 -1.3 2,707,199 + CCNA_02560 0.54 +0.8 2,707,240 - CCNA_02560 0.60 +2.5 2,707,317 + CCNA_02560 0.72 -0.7 2,707,318 - CCNA_02560 0.73 +1.1 2,707,318 - CCNA_02560 0.73 +1.2 2,707,318 - CCNA_02560 0.73 +2.3 2,707,358 + CCNA_02560 0.79 +0.2 2,707,361 - CCNA_02560 0.79 +0.2 2,707,361 - CCNA_02560 0.79 +1.5 2,707,361 - CCNA_02560 0.79 +1.9 2,707,405 + CCNA_02560 0.86 -0.2 2,707,407 + CCNA_02560 0.87 +0.2 2,707,407 + CCNA_02560 0.87 -0.8 2,707,408 - CCNA_02560 0.87 +1.0 2,707,408 - CCNA_02560 0.87 +3.3 2,707,415 - CCNA_02560 0.88 +3.3 2,707,415 - CCNA_02560 0.88 +0.3 2,707,415 - CCNA_02560 0.88 +1.8 2,707,415 - CCNA_02560 0.88 +4.0 2,707,415 - CCNA_02560 0.88 +1.0 2,707,428 + CCNA_02560 0.90 +1.2 2,707,430 + +0.8 2,707,430 + -2.6 2,707,436 - +1.4 2,707,456 + +2.8 2,707,480 - +0.8 2,707,480 - +3.4 2,707,482 + -0.2 2,707,512 - +1.6 2,707,512 - +2.2 2,707,563 + -0.4 2,707,601 + +0.4 2,707,601 + -1.3 2,707,609 - +3.4 2,707,633 + CCNA_02561 0.10 +0.8 2,707,651 - CCNA_02561 0.11 +1.9 2,707,699 - CCNA_02561 0.15 +0.4 2,707,703 + CCNA_02561 0.15 -1.4 2,707,703 + CCNA_02561 0.15 -0.4 2,707,711 - CCNA_02561 0.16 +3.1 2,707,724 + CCNA_02561 0.16 -0.2 2,707,724 + CCNA_02561 0.16 +1.8 2,707,725 + CCNA_02561 0.17 -0.8 2,707,747 + CCNA_02561 0.18 +0.2 2,707,755 - CCNA_02561 0.19 +1.8 2,707,755 - CCNA_02561 0.19 +1.4 2,707,765 - CCNA_02561 0.19 +1.4 2,707,777 - CCNA_02561 0.20 +2.1 2,707,847 + CCNA_02561 0.25 +0.8 2,707,868 + CCNA_02561 0.27 -0.6 2,707,868 + CCNA_02561 0.27 +0.8 2,707,876 - CCNA_02561 0.27 -0.9 2,707,880 + CCNA_02561 0.27 -0.0 2,707,888 - CCNA_02561 0.28 +1.4 2,707,888 - CCNA_02561 0.28 +2.5 2,707,896 + CCNA_02561 0.29 +1.2 2,707,946 - CCNA_02561 0.32 +1.2 2,708,020 - CCNA_02561 0.37 +1.5 2,708,026 - CCNA_02561 0.38 +2.4 2,708,039 - CCNA_02561 0.39 +2.1 2,708,216 + CCNA_02561 0.51 +1.2 2,708,251 - CCNA_02561 0.53 +2.2 2,708,256 - CCNA_02561 0.54 +2.1 2,708,256 - CCNA_02561 0.54 +1.3 2,708,259 + CCNA_02561 0.54 -0.7 2,708,277 - CCNA_02561 0.55 +1.8 2,708,364 - CCNA_02561 0.61 +1.3 2,708,366 + CCNA_02561 0.62 +1.8 2,708,397 - CCNA_02561 0.64 +2.4 2,708,406 - CCNA_02561 0.64 +2.8 2,708,406 - CCNA_02561 0.64 +3.6 2,708,406 - CCNA_02561 0.64 +2.0 2,708,435 + CCNA_02561 0.66 +1.2 2,708,435 + CCNA_02561 0.66 +0.1
Or see this region's nucleotide sequence