Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02174 and CCNA_02175 are separated by 10 nucleotides CCNA_02175 and CCNA_02176 are separated by 14 nucleotides CCNA_02176 and CCNA_02177 are separated by 78 nucleotides CCNA_02177 and CCNA_02178 are separated by 63 nucleotides
CCNA_02174: CCNA_02174 - multidrug resistance efflux pump, at 2,326,862 to 2,327,866
_02174
CCNA_02175: CCNA_02175 - hypothetical protein, at 2,327,877 to 2,328,401
_02175
CCNA_02176: CCNA_02176 - hypothetical protein, at 2,328,416 to 2,328,604
_02176
CCNA_02177: CCNA_02177 - hypothetical protein, at 2,328,683 to 2,328,937
_02177
CCNA_02178: CCNA_02178 - short-chain alcohol dehydrogenase, at 2,329,001 to 2,329,906
_02178
Position (kb)
2328
2329 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 2327.691 kb on + strand, within CCNA_02174 at 2327.691 kb on + strand, within CCNA_02174 at 2327.699 kb on - strand, within CCNA_02174 at 2327.699 kb on - strand, within CCNA_02174 at 2327.699 kb on - strand, within CCNA_02174 at 2327.734 kb on - strand, within CCNA_02174 at 2327.734 kb on - strand, within CCNA_02174 at 2327.862 kb on + strand at 2327.868 kb on - strand at 2327.889 kb on + strand at 2327.889 kb on + strand at 2327.889 kb on + strand at 2327.897 kb on - strand at 2327.900 kb on - strand at 2327.929 kb on - strand at 2327.936 kb on + strand, within CCNA_02175 at 2327.936 kb on + strand, within CCNA_02175 at 2327.944 kb on - strand, within CCNA_02175 at 2327.949 kb on + strand, within CCNA_02175 at 2327.950 kb on + strand, within CCNA_02175 at 2327.954 kb on + strand, within CCNA_02175 at 2328.003 kb on - strand, within CCNA_02175 at 2328.015 kb on - strand, within CCNA_02175 at 2328.167 kb on + strand, within CCNA_02175 at 2328.189 kb on + strand, within CCNA_02175 at 2328.189 kb on + strand, within CCNA_02175 at 2328.189 kb on + strand, within CCNA_02175 at 2328.197 kb on - strand, within CCNA_02175 at 2328.197 kb on - strand, within CCNA_02175 at 2328.197 kb on - strand, within CCNA_02175 at 2328.238 kb on + strand, within CCNA_02175 at 2328.255 kb on - strand, within CCNA_02175 at 2328.328 kb on - strand, within CCNA_02175 at 2328.379 kb on - strand at 2328.402 kb on - strand at 2328.519 kb on + strand, within CCNA_02176 at 2328.541 kb on - strand, within CCNA_02176 at 2328.685 kb on - strand at 2328.685 kb on - strand at 2328.701 kb on - strand at 2328.754 kb on + strand, within CCNA_02177 at 2328.887 kb on - strand, within CCNA_02177 at 2328.931 kb on + strand at 2328.939 kb on - strand at 2328.941 kb on + strand at 2328.962 kb on - strand at 2329.045 kb on + strand at 2329.070 kb on - strand at 2329.074 kb on + strand at 2329.082 kb on - strand at 2329.123 kb on - strand, within CCNA_02178 at 2329.165 kb on + strand, within CCNA_02178 at 2329.168 kb on - strand, within CCNA_02178 at 2329.180 kb on + strand, within CCNA_02178 at 2329.244 kb on + strand, within CCNA_02178 at 2329.269 kb on - strand, within CCNA_02178 at 2329.280 kb on - strand, within CCNA_02178 at 2329.280 kb on - strand, within CCNA_02178 at 2329.280 kb on - strand, within CCNA_02178 at 2329.311 kb on - strand, within CCNA_02178 at 2329.330 kb on + strand, within CCNA_02178 at 2329.360 kb on + strand, within CCNA_02178 at 2329.360 kb on + strand, within CCNA_02178 at 2329.368 kb on - strand, within CCNA_02178 at 2329.377 kb on - strand, within CCNA_02178 at 2329.378 kb on + strand, within CCNA_02178 at 2329.386 kb on + strand, within CCNA_02178 at 2329.386 kb on + strand, within CCNA_02178 at 2329.396 kb on + strand, within CCNA_02178 at 2329.396 kb on + strand, within CCNA_02178 at 2329.396 kb on + strand, within CCNA_02178 at 2329.396 kb on + strand, within CCNA_02178 at 2329.404 kb on - strand, within CCNA_02178 at 2329.406 kb on + strand, within CCNA_02178 at 2329.424 kb on + strand, within CCNA_02178 at 2329.432 kb on - strand, within CCNA_02178 at 2329.432 kb on - strand, within CCNA_02178 at 2329.432 kb on - strand, within CCNA_02178 at 2329.517 kb on + strand, within CCNA_02178 at 2329.570 kb on + strand, within CCNA_02178 at 2329.578 kb on - strand, within CCNA_02178 at 2329.616 kb on - strand, within CCNA_02178 at 2329.631 kb on + strand, within CCNA_02178 at 2329.683 kb on + strand, within CCNA_02178 at 2329.702 kb on - strand, within CCNA_02178 at 2329.717 kb on - strand, within CCNA_02178 at 2329.756 kb on + strand, within CCNA_02178 at 2329.764 kb on - strand, within CCNA_02178 at 2329.772 kb on - strand, within CCNA_02178 at 2329.802 kb on - strand, within CCNA_02178 at 2329.812 kb on + strand, within CCNA_02178 at 2329.822 kb on + strand at 2329.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 2,327,691 + CCNA_02174 0.82 -5.0 2,327,691 + CCNA_02174 0.82 -2.4 2,327,699 - CCNA_02174 0.83 -1.3 2,327,699 - CCNA_02174 0.83 -0.4 2,327,699 - CCNA_02174 0.83 -1.2 2,327,734 - CCNA_02174 0.87 -3.7 2,327,734 - CCNA_02174 0.87 -1.1 2,327,862 + +2.5 2,327,868 - +0.7 2,327,889 + +0.4 2,327,889 + +2.3 2,327,889 + +2.1 2,327,897 - -0.2 2,327,900 - +0.4 2,327,929 - -0.4 2,327,936 + CCNA_02175 0.11 +1.7 2,327,936 + CCNA_02175 0.11 +0.9 2,327,944 - CCNA_02175 0.13 -1.9 2,327,949 + CCNA_02175 0.14 -0.5 2,327,950 + CCNA_02175 0.14 +1.1 2,327,954 + CCNA_02175 0.15 +1.5 2,328,003 - CCNA_02175 0.24 -1.3 2,328,015 - CCNA_02175 0.26 -1.4 2,328,167 + CCNA_02175 0.55 +2.4 2,328,189 + CCNA_02175 0.59 -1.7 2,328,189 + CCNA_02175 0.59 -1.1 2,328,189 + CCNA_02175 0.59 +0.7 2,328,197 - CCNA_02175 0.61 -0.9 2,328,197 - CCNA_02175 0.61 -1.1 2,328,197 - CCNA_02175 0.61 -1.7 2,328,238 + CCNA_02175 0.69 +0.4 2,328,255 - CCNA_02175 0.72 -0.6 2,328,328 - CCNA_02175 0.86 -0.8 2,328,379 - -0.6 2,328,402 - -0.3 2,328,519 + CCNA_02176 0.54 +1.1 2,328,541 - CCNA_02176 0.66 +0.1 2,328,685 - +1.4 2,328,685 - -0.9 2,328,701 - -1.3 2,328,754 + CCNA_02177 0.28 -1.8 2,328,887 - CCNA_02177 0.80 -2.3 2,328,931 + +1.4 2,328,939 - -0.7 2,328,941 + +3.1 2,328,962 - -0.8 2,329,045 + +0.5 2,329,070 - -1.2 2,329,074 + +0.6 2,329,082 - -2.1 2,329,123 - CCNA_02178 0.13 -1.8 2,329,165 + CCNA_02178 0.18 -0.8 2,329,168 - CCNA_02178 0.18 -1.3 2,329,180 + CCNA_02178 0.20 -0.2 2,329,244 + CCNA_02178 0.27 -2.1 2,329,269 - CCNA_02178 0.30 -0.4 2,329,280 - CCNA_02178 0.31 +0.1 2,329,280 - CCNA_02178 0.31 -1.5 2,329,280 - CCNA_02178 0.31 +0.1 2,329,311 - CCNA_02178 0.34 -0.4 2,329,330 + CCNA_02178 0.36 +1.4 2,329,360 + CCNA_02178 0.40 -0.4 2,329,360 + CCNA_02178 0.40 +0.1 2,329,368 - CCNA_02178 0.41 -1.9 2,329,377 - CCNA_02178 0.42 +0.3 2,329,378 + CCNA_02178 0.42 -0.1 2,329,386 + CCNA_02178 0.42 -0.2 2,329,386 + CCNA_02178 0.42 -2.0 2,329,396 + CCNA_02178 0.44 -0.3 2,329,396 + CCNA_02178 0.44 +0.7 2,329,396 + CCNA_02178 0.44 -0.2 2,329,396 + CCNA_02178 0.44 -0.8 2,329,404 - CCNA_02178 0.44 +0.8 2,329,406 + CCNA_02178 0.45 +0.4 2,329,424 + CCNA_02178 0.47 -0.4 2,329,432 - CCNA_02178 0.48 +0.1 2,329,432 - CCNA_02178 0.48 -0.2 2,329,432 - CCNA_02178 0.48 -1.6 2,329,517 + CCNA_02178 0.57 -0.4 2,329,570 + CCNA_02178 0.63 -0.5 2,329,578 - CCNA_02178 0.64 +0.1 2,329,616 - CCNA_02178 0.68 -1.8 2,329,631 + CCNA_02178 0.70 -0.1 2,329,683 + CCNA_02178 0.75 +0.9 2,329,702 - CCNA_02178 0.77 -1.2 2,329,717 - CCNA_02178 0.79 -0.4 2,329,756 + CCNA_02178 0.83 +2.2 2,329,764 - CCNA_02178 0.84 -0.4 2,329,772 - CCNA_02178 0.85 -2.5 2,329,802 - CCNA_02178 0.88 +0.1 2,329,812 + CCNA_02178 0.90 +3.2 2,329,822 + +5.0 2,329,825 - -0.8
Or see this region's nucleotide sequence