Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00725

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00723 and CCNA_00724 are separated by 18 nucleotidesCCNA_00724 and CCNA_00725 are separated by 200 nucleotidesCCNA_00725 and CCNA_00726 are separated by 50 nucleotides CCNA_00723: CCNA_00723 - glyoxalase family protein, at 780,108 to 780,302 _00723 CCNA_00724: CCNA_00724 - hypothetical protein, at 780,321 to 780,749 _00724 CCNA_00725: CCNA_00725 - O6-methylguanine-DNA methyltransferase, at 780,950 to 781,612 _00725 CCNA_00726: CCNA_00726 - acyl-CoA dehydrogenase, at 781,663 to 782,970 _00726 Position (kb) 780 781 782Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 779.990 kb on - strandat 780.040 kb on - strandat 780.075 kb on + strandat 780.090 kb on + strandat 780.090 kb on + strandat 780.090 kb on + strandat 780.090 kb on + strandat 780.090 kb on + strandat 780.090 kb on + strandat 780.090 kb on + strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.098 kb on - strandat 780.114 kb on + strandat 780.116 kb on + strandat 780.122 kb on - strandat 780.124 kb on - strandat 780.132 kb on - strand, within CCNA_00723at 780.141 kb on + strand, within CCNA_00723at 780.141 kb on + strand, within CCNA_00723at 780.202 kb on + strand, within CCNA_00723at 780.233 kb on + strand, within CCNA_00723at 780.233 kb on + strand, within CCNA_00723at 780.235 kb on + strand, within CCNA_00723at 780.241 kb on - strand, within CCNA_00723at 780.241 kb on - strand, within CCNA_00723at 780.241 kb on - strand, within CCNA_00723at 780.286 kb on + strandat 780.316 kb on - strandat 780.344 kb on + strandat 780.391 kb on - strand, within CCNA_00724at 780.416 kb on + strand, within CCNA_00724at 780.416 kb on + strand, within CCNA_00724at 780.416 kb on + strand, within CCNA_00724at 780.422 kb on - strand, within CCNA_00724at 780.424 kb on + strand, within CCNA_00724at 780.424 kb on - strand, within CCNA_00724at 780.424 kb on - strand, within CCNA_00724at 780.434 kb on + strand, within CCNA_00724at 780.434 kb on + strand, within CCNA_00724at 780.448 kb on + strand, within CCNA_00724at 780.456 kb on - strand, within CCNA_00724at 780.456 kb on - strand, within CCNA_00724at 780.456 kb on - strand, within CCNA_00724at 780.456 kb on - strand, within CCNA_00724at 780.485 kb on + strand, within CCNA_00724at 780.485 kb on + strand, within CCNA_00724at 780.519 kb on - strand, within CCNA_00724at 780.521 kb on + strand, within CCNA_00724at 780.530 kb on - strand, within CCNA_00724at 780.534 kb on - strand, within CCNA_00724at 780.555 kb on + strand, within CCNA_00724at 780.561 kb on + strand, within CCNA_00724at 780.561 kb on + strand, within CCNA_00724at 780.561 kb on + strand, within CCNA_00724at 780.561 kb on + strand, within CCNA_00724at 780.561 kb on + strand, within CCNA_00724at 780.562 kb on + strand, within CCNA_00724at 780.563 kb on - strand, within CCNA_00724at 780.569 kb on - strand, within CCNA_00724at 780.570 kb on - strand, within CCNA_00724at 780.570 kb on - strand, within CCNA_00724at 780.575 kb on - strand, within CCNA_00724at 780.583 kb on + strand, within CCNA_00724at 780.657 kb on + strand, within CCNA_00724at 780.670 kb on - strand, within CCNA_00724at 780.677 kb on - strand, within CCNA_00724at 780.682 kb on - strand, within CCNA_00724at 780.703 kb on - strand, within CCNA_00724at 780.737 kb on + strandat 780.738 kb on + strandat 780.743 kb on + strandat 780.743 kb on + strandat 780.751 kb on - strandat 780.751 kb on - strandat 780.860 kb on + strandat 780.882 kb on + strandat 780.948 kb on + strandat 780.956 kb on - strandat 780.989 kb on + strandat 781.079 kb on - strand, within CCNA_00725at 781.124 kb on + strand, within CCNA_00725at 781.129 kb on + strand, within CCNA_00725at 781.129 kb on + strand, within CCNA_00725at 781.137 kb on - strand, within CCNA_00725at 781.137 kb on - strand, within CCNA_00725at 781.137 kb on - strand, within CCNA_00725at 781.148 kb on - strand, within CCNA_00725at 781.166 kb on + strand, within CCNA_00725at 781.174 kb on - strand, within CCNA_00725at 781.220 kb on - strand, within CCNA_00725at 781.220 kb on - strand, within CCNA_00725at 781.225 kb on - strand, within CCNA_00725at 781.317 kb on - strand, within CCNA_00725at 781.356 kb on - strand, within CCNA_00725at 781.394 kb on - strand, within CCNA_00725at 781.409 kb on - strand, within CCNA_00725at 781.418 kb on - strand, within CCNA_00725at 781.450 kb on - strand, within CCNA_00725at 781.481 kb on - strand, within CCNA_00725at 781.513 kb on + strand, within CCNA_00725at 781.613 kb on - strandat 781.689 kb on - strandat 781.689 kb on - strandat 781.717 kb on + strandat 781.718 kb on - strandat 781.736 kb on + strandat 781.762 kb on - strandat 781.770 kb on - strandat 781.772 kb on - strandat 781.783 kb on - strandat 781.797 kb on + strand, within CCNA_00726at 781.817 kb on + strand, within CCNA_00726at 781.817 kb on + strand, within CCNA_00726at 781.825 kb on - strand, within CCNA_00726at 781.825 kb on - strand, within CCNA_00726at 781.825 kb on - strand, within CCNA_00726at 781.827 kb on + strand, within CCNA_00726at 781.827 kb on + strand, within CCNA_00726at 781.835 kb on - strand, within CCNA_00726at 781.835 kb on - strand, within CCNA_00726at 781.835 kb on - strand, within CCNA_00726at 781.848 kb on - strand, within CCNA_00726at 781.861 kb on + strand, within CCNA_00726at 781.897 kb on + strand, within CCNA_00726at 781.910 kb on + strand, within CCNA_00726at 781.918 kb on - strand, within CCNA_00726at 781.923 kb on - strand, within CCNA_00726at 781.934 kb on + strand, within CCNA_00726at 781.981 kb on - strand, within CCNA_00726at 782.022 kb on - strand, within CCNA_00726at 782.036 kb on - strand, within CCNA_00726at 782.040 kb on - strand, within CCNA_00726at 782.063 kb on + strand, within CCNA_00726at 782.070 kb on + strand, within CCNA_00726at 782.164 kb on - strand, within CCNA_00726at 782.175 kb on - strand, within CCNA_00726at 782.175 kb on - strand, within CCNA_00726at 782.186 kb on - strand, within CCNA_00726at 782.210 kb on - strand, within CCNA_00726at 782.234 kb on + strand, within CCNA_00726at 782.254 kb on - strand, within CCNA_00726at 782.260 kb on - strand, within CCNA_00726at 782.265 kb on - strand, within CCNA_00726at 782.279 kb on - strandat 782.279 kb on - strand, within CCNA_00726at 782.349 kb on - strand, within CCNA_00726at 782.386 kb on - strand, within CCNA_00726at 782.421 kb on + strand, within CCNA_00726at 782.429 kb on - strand, within CCNA_00726at 782.429 kb on - strand, within CCNA_00726at 782.483 kb on - strand, within CCNA_00726at 782.490 kb on - strand, within CCNA_00726at 782.490 kb on - strand, within CCNA_00726at 782.507 kb on - strand, within CCNA_00726at 782.507 kb on - strand, within CCNA_00726at 782.512 kb on - strand, within CCNA_00726at 782.517 kb on - strand, within CCNA_00726at 782.525 kb on + strand, within CCNA_00726

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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779,990 - +2.2
780,040 - +4.5
780,075 + -1.8
780,090 + -0.9
780,090 + -0.8
780,090 + +0.2
780,090 + +2.3
780,090 + -0.2
780,090 + -0.3
780,090 + +0.7
780,098 - +0.7
780,098 - +0.6
780,098 - +1.0
780,098 - +1.0
780,098 - +0.8
780,098 - -1.0
780,098 - +2.5
780,098 - +0.7
780,098 - +1.3
780,098 - -1.0
780,098 - +2.6
780,098 - -0.1
780,114 + -0.1
780,116 + +2.7
780,122 - +0.3
780,124 - -1.1
780,132 - CCNA_00723 0.12 -0.5
780,141 + CCNA_00723 0.17 -1.9
780,141 + CCNA_00723 0.17 +0.6
780,202 + CCNA_00723 0.48 -2.2
780,233 + CCNA_00723 0.64 +2.2
780,233 + CCNA_00723 0.64 -0.3
780,235 + CCNA_00723 0.65 -1.7
780,241 - CCNA_00723 0.68 -0.8
780,241 - CCNA_00723 0.68 -0.2
780,241 - CCNA_00723 0.68 +0.6
780,286 + +0.0
780,316 - -0.7
780,344 + +1.3
780,391 - CCNA_00724 0.16 +0.7
780,416 + CCNA_00724 0.22 +0.7
780,416 + CCNA_00724 0.22 -1.1
780,416 + CCNA_00724 0.22 -0.0
780,422 - CCNA_00724 0.24 +0.6
780,424 + CCNA_00724 0.24 +0.8
780,424 - CCNA_00724 0.24 +0.3
780,424 - CCNA_00724 0.24 +0.9
780,434 + CCNA_00724 0.26 +0.3
780,434 + CCNA_00724 0.26 -0.9
780,448 + CCNA_00724 0.30 +1.9
780,456 - CCNA_00724 0.31 -1.0
780,456 - CCNA_00724 0.31 -0.4
780,456 - CCNA_00724 0.31 +3.9
780,456 - CCNA_00724 0.31 +1.3
780,485 + CCNA_00724 0.38 +2.8
780,485 + CCNA_00724 0.38 +0.4
780,519 - CCNA_00724 0.46 +1.3
780,521 + CCNA_00724 0.47 +0.3
780,530 - CCNA_00724 0.49 -0.4
780,534 - CCNA_00724 0.50 +1.6
780,555 + CCNA_00724 0.55 +1.0
780,561 + CCNA_00724 0.56 -0.3
780,561 + CCNA_00724 0.56 +0.8
780,561 + CCNA_00724 0.56 -0.1
780,561 + CCNA_00724 0.56 +0.0
780,561 + CCNA_00724 0.56 +0.9
780,562 + CCNA_00724 0.56 +0.1
780,563 - CCNA_00724 0.56 -0.4
780,569 - CCNA_00724 0.58 -0.0
780,570 - CCNA_00724 0.58 +0.9
780,570 - CCNA_00724 0.58 -0.8
780,575 - CCNA_00724 0.59 -0.3
780,583 + CCNA_00724 0.61 +2.3
780,657 + CCNA_00724 0.78 +2.9
780,670 - CCNA_00724 0.81 +0.3
780,677 - CCNA_00724 0.83 -0.2
780,682 - CCNA_00724 0.84 +1.2
780,703 - CCNA_00724 0.89 +1.3
780,737 + +1.0
780,738 + +2.1
780,743 + +2.0
780,743 + +2.4
780,751 - -1.6
780,751 - +2.9
780,860 + +0.5
780,882 + +1.3
780,948 + -0.4
780,956 - +0.6
780,989 + +3.1
781,079 - CCNA_00725 0.19 -0.2
781,124 + CCNA_00725 0.26 +2.2
781,129 + CCNA_00725 0.27 -2.4
781,129 + CCNA_00725 0.27 -0.3
781,137 - CCNA_00725 0.28 -0.8
781,137 - CCNA_00725 0.28 -0.3
781,137 - CCNA_00725 0.28 -0.4
781,148 - CCNA_00725 0.30 +0.1
781,166 + CCNA_00725 0.33 +0.9
781,174 - CCNA_00725 0.34 +2.8
781,220 - CCNA_00725 0.41 +0.6
781,220 - CCNA_00725 0.41 -1.3
781,225 - CCNA_00725 0.41 +0.3
781,317 - CCNA_00725 0.55 -2.0
781,356 - CCNA_00725 0.61 +0.6
781,394 - CCNA_00725 0.67 -1.8
781,409 - CCNA_00725 0.69 -0.8
781,418 - CCNA_00725 0.71 -1.0
781,450 - CCNA_00725 0.75 -1.2
781,481 - CCNA_00725 0.80 +0.6
781,513 + CCNA_00725 0.85 +0.1
781,613 - +0.8
781,689 - +2.4
781,689 - +2.4
781,717 + +1.3
781,718 - +0.3
781,736 + -0.1
781,762 - +2.9
781,770 - +2.8
781,772 - +2.8
781,783 - +2.9
781,797 + CCNA_00726 0.10 +0.6
781,817 + CCNA_00726 0.12 +0.3
781,817 + CCNA_00726 0.12 +0.5
781,825 - CCNA_00726 0.12 +1.3
781,825 - CCNA_00726 0.12 +2.5
781,825 - CCNA_00726 0.12 +4.3
781,827 + CCNA_00726 0.13 -0.3
781,827 + CCNA_00726 0.13 +0.5
781,835 - CCNA_00726 0.13 +2.9
781,835 - CCNA_00726 0.13 +3.3
781,835 - CCNA_00726 0.13 +2.3
781,848 - CCNA_00726 0.14 +2.7
781,861 + CCNA_00726 0.15 +2.9
781,897 + CCNA_00726 0.18 -0.5
781,910 + CCNA_00726 0.19 -1.0
781,918 - CCNA_00726 0.19 +2.0
781,923 - CCNA_00726 0.20 +2.9
781,934 + CCNA_00726 0.21 +0.3
781,981 - CCNA_00726 0.24 +3.1
782,022 - CCNA_00726 0.27 +1.2
782,036 - CCNA_00726 0.29 +3.2
782,040 - CCNA_00726 0.29 +2.2
782,063 + CCNA_00726 0.31 -0.8
782,070 + CCNA_00726 0.31 +1.2
782,164 - CCNA_00726 0.38 +2.2
782,175 - CCNA_00726 0.39 +1.6
782,175 - CCNA_00726 0.39 +2.3
782,186 - CCNA_00726 0.40 +2.5
782,210 - CCNA_00726 0.42 +1.1
782,234 + CCNA_00726 0.44 +1.3
782,254 - CCNA_00726 0.45 +2.5
782,260 - CCNA_00726 0.46 +1.4
782,265 - CCNA_00726 0.46 +2.5
782,279 - +1.9
782,279 - CCNA_00726 0.47 +2.1
782,349 - CCNA_00726 0.52 +3.2
782,386 - CCNA_00726 0.55 +3.0
782,421 + CCNA_00726 0.58 +0.9
782,429 - CCNA_00726 0.59 +2.7
782,429 - CCNA_00726 0.59 +2.2
782,483 - CCNA_00726 0.63 +2.6
782,490 - CCNA_00726 0.63 +1.9
782,490 - CCNA_00726 0.63 +2.5
782,507 - CCNA_00726 0.65 +3.3
782,507 - CCNA_00726 0.65 +1.9
782,512 - CCNA_00726 0.65 +2.9
782,517 - CCNA_00726 0.65 +1.1
782,525 + CCNA_00726 0.66 +0.9

Or see this region's nucleotide sequence