Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00508

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00506 and CCNA_00507 are separated by 5 nucleotidesCCNA_00507 and CCNA_00508 are separated by 67 nucleotidesCCNA_00508 and CCNA_00509 are separated by 65 nucleotidesCCNA_00509 and CCNA_00510 overlap by 4 nucleotides CCNA_00506: CCNA_00506 - cytochrome b, at 520,955 to 522,235 _00506 CCNA_00507: CCNA_00507 - cytochrome c1, at 522,241 to 523,086 _00507 CCNA_00508: CCNA_00508 - aspartate protease, at 523,154 to 523,927 _00508 CCNA_00509: CCNA_00509 - phosphoglycerate mutase family, at 523,993 to 524,526 _00509 CCNA_00510: CCNA_00510 - carboxymethylenebutenolidase, at 524,523 to 525,200 _00510 Position (kb) 523 524Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 522.277 kb on + strandat 522.375 kb on + strand, within CCNA_00507at 522.375 kb on + strand, within CCNA_00507at 522.418 kb on + strand, within CCNA_00507at 522.519 kb on + strand, within CCNA_00507at 522.707 kb on + strand, within CCNA_00507at 522.717 kb on + strand, within CCNA_00507at 522.740 kb on + strand, within CCNA_00507at 522.770 kb on + strand, within CCNA_00507at 522.783 kb on - strand, within CCNA_00507at 522.933 kb on + strand, within CCNA_00507at 522.933 kb on + strand, within CCNA_00507at 522.977 kb on + strand, within CCNA_00507at 522.977 kb on + strand, within CCNA_00507at 522.985 kb on - strand, within CCNA_00507at 523.019 kb on + strandat 523.041 kb on + strandat 523.049 kb on - strandat 523.172 kb on + strandat 523.252 kb on + strand, within CCNA_00508at 523.252 kb on + strand, within CCNA_00508at 523.252 kb on + strand, within CCNA_00508at 523.260 kb on - strand, within CCNA_00508at 523.260 kb on - strand, within CCNA_00508at 523.278 kb on + strand, within CCNA_00508at 523.369 kb on - strand, within CCNA_00508at 523.491 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.518 kb on + strand, within CCNA_00508at 523.523 kb on + strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.526 kb on - strand, within CCNA_00508at 523.586 kb on - strand, within CCNA_00508at 523.735 kb on + strand, within CCNA_00508at 523.748 kb on - strand, within CCNA_00508at 523.748 kb on - strand, within CCNA_00508at 523.755 kb on - strand, within CCNA_00508at 523.778 kb on - strand, within CCNA_00508at 523.814 kb on + strand, within CCNA_00508at 523.836 kb on + strand, within CCNA_00508at 523.844 kb on - strand, within CCNA_00508at 523.878 kb on + strandat 523.883 kb on + strandat 523.886 kb on - strandat 523.886 kb on - strandat 523.891 kb on - strandat 523.931 kb on - strandat 523.935 kb on + strandat 523.945 kb on + strandat 523.945 kb on + strandat 523.945 kb on + strandat 523.953 kb on - strandat 523.953 kb on - strandat 523.953 kb on - strandat 523.970 kb on + strandat 523.988 kb on - strandat 524.008 kb on + strandat 524.008 kb on + strandat 524.008 kb on + strandat 524.024 kb on + strandat 524.058 kb on + strand, within CCNA_00509at 524.117 kb on + strand, within CCNA_00509at 524.125 kb on - strand, within CCNA_00509at 524.150 kb on + strand, within CCNA_00509at 524.150 kb on + strand, within CCNA_00509at 524.150 kb on + strand, within CCNA_00509at 524.150 kb on + strand, within CCNA_00509at 524.156 kb on - strand, within CCNA_00509at 524.158 kb on - strand, within CCNA_00509at 524.178 kb on - strand, within CCNA_00509at 524.185 kb on + strand, within CCNA_00509at 524.185 kb on - strand, within CCNA_00509at 524.193 kb on - strand, within CCNA_00509at 524.193 kb on - strand, within CCNA_00509at 524.220 kb on + strand, within CCNA_00509at 524.223 kb on - strand, within CCNA_00509at 524.228 kb on - strand, within CCNA_00509at 524.282 kb on + strand, within CCNA_00509at 524.287 kb on + strand, within CCNA_00509at 524.304 kb on - strand, within CCNA_00509at 524.317 kb on - strand, within CCNA_00509at 524.331 kb on - strand, within CCNA_00509at 524.341 kb on - strand, within CCNA_00509at 524.363 kb on + strand, within CCNA_00509at 524.369 kb on + strand, within CCNA_00509at 524.409 kb on - strand, within CCNA_00509at 524.417 kb on + strand, within CCNA_00509at 524.417 kb on + strand, within CCNA_00509at 524.493 kb on + strandat 524.494 kb on + strandat 524.494 kb on + strandat 524.494 kb on + strandat 524.494 kb on + strandat 524.502 kb on - strandat 524.561 kb on + strandat 524.561 kb on + strandat 524.569 kb on - strandat 524.569 kb on - strandat 524.577 kb on - strandat 524.620 kb on + strand, within CCNA_00510at 524.625 kb on + strand, within CCNA_00510at 524.625 kb on + strand, within CCNA_00510at 524.625 kb on + strand, within CCNA_00510at 524.638 kb on - strand, within CCNA_00510at 524.643 kb on - strand, within CCNA_00510at 524.648 kb on - strand, within CCNA_00510at 524.713 kb on + strand, within CCNA_00510at 524.724 kb on + strand, within CCNA_00510at 524.724 kb on + strand, within CCNA_00510at 524.724 kb on + strand, within CCNA_00510at 524.724 kb on + strand, within CCNA_00510at 524.732 kb on - strand, within CCNA_00510at 524.748 kb on - strand, within CCNA_00510at 524.795 kb on + strand, within CCNA_00510at 524.805 kb on - strand, within CCNA_00510at 524.855 kb on + strand, within CCNA_00510at 524.863 kb on + strand, within CCNA_00510at 524.863 kb on + strand, within CCNA_00510at 524.870 kb on - strand, within CCNA_00510at 524.871 kb on - strand, within CCNA_00510at 524.895 kb on + strand, within CCNA_00510at 524.895 kb on + strand, within CCNA_00510at 524.907 kb on + strand, within CCNA_00510at 524.922 kb on + strand, within CCNA_00510

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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522,277 + +3.3
522,375 + CCNA_00507 0.16 +0.2
522,375 + CCNA_00507 0.16 +1.4
522,418 + CCNA_00507 0.21 +2.1
522,519 + CCNA_00507 0.33 +2.4
522,707 + CCNA_00507 0.55 +1.5
522,717 + CCNA_00507 0.56 +2.3
522,740 + CCNA_00507 0.59 +2.6
522,770 + CCNA_00507 0.63 +1.1
522,783 - CCNA_00507 0.64 -0.3
522,933 + CCNA_00507 0.82 +1.2
522,933 + CCNA_00507 0.82 +2.2
522,977 + CCNA_00507 0.87 +3.1
522,977 + CCNA_00507 0.87 +0.1
522,985 - CCNA_00507 0.88 +0.4
523,019 + +3.1
523,041 + +3.6
523,049 - +1.1
523,172 + +1.8
523,252 + CCNA_00508 0.13 +1.2
523,252 + CCNA_00508 0.13 +2.7
523,252 + CCNA_00508 0.13 +1.8
523,260 - CCNA_00508 0.14 +0.7
523,260 - CCNA_00508 0.14 -1.2
523,278 + CCNA_00508 0.16 +2.1
523,369 - CCNA_00508 0.28 +0.3
523,491 + CCNA_00508 0.44 +0.5
523,518 + CCNA_00508 0.47 -0.4
523,518 + CCNA_00508 0.47 -1.6
523,518 + CCNA_00508 0.47 -0.8
523,518 + CCNA_00508 0.47 -2.7
523,518 + CCNA_00508 0.47 +1.7
523,518 + CCNA_00508 0.47 +1.2
523,518 + CCNA_00508 0.47 -0.2
523,518 + CCNA_00508 0.47 +1.9
523,518 + CCNA_00508 0.47 +1.7
523,518 + CCNA_00508 0.47 -1.2
523,518 + CCNA_00508 0.47 -1.3
523,523 + CCNA_00508 0.48 +0.6
523,526 - CCNA_00508 0.48 +0.1
523,526 - CCNA_00508 0.48 +1.0
523,526 - CCNA_00508 0.48 +0.2
523,526 - CCNA_00508 0.48 +1.5
523,526 - CCNA_00508 0.48 +2.0
523,526 - CCNA_00508 0.48 +0.4
523,526 - CCNA_00508 0.48 -1.1
523,526 - CCNA_00508 0.48 +1.2
523,526 - CCNA_00508 0.48 +1.0
523,526 - CCNA_00508 0.48 +2.8
523,526 - CCNA_00508 0.48 -0.0
523,586 - CCNA_00508 0.56 +1.4
523,735 + CCNA_00508 0.75 +1.6
523,748 - CCNA_00508 0.77 +1.0
523,748 - CCNA_00508 0.77 -0.5
523,755 - CCNA_00508 0.78 +0.1
523,778 - CCNA_00508 0.81 +0.4
523,814 + CCNA_00508 0.85 -0.3
523,836 + CCNA_00508 0.88 +0.9
523,844 - CCNA_00508 0.89 +1.1
523,878 + -0.9
523,883 + -2.3
523,886 - -1.4
523,886 - +2.3
523,891 - +0.6
523,931 - +0.3
523,935 + -1.1
523,945 + +0.7
523,945 + +1.5
523,945 + -0.2
523,953 - +2.3
523,953 - -0.1
523,953 - -0.3
523,970 + +1.2
523,988 - -1.1
524,008 + +0.9
524,008 + +0.7
524,008 + +1.0
524,024 + -0.9
524,058 + CCNA_00509 0.12 +0.7
524,117 + CCNA_00509 0.23 -0.8
524,125 - CCNA_00509 0.25 -2.8
524,150 + CCNA_00509 0.29 -0.8
524,150 + CCNA_00509 0.29 -1.2
524,150 + CCNA_00509 0.29 +1.5
524,150 + CCNA_00509 0.29 -1.1
524,156 - CCNA_00509 0.31 -2.6
524,158 - CCNA_00509 0.31 +2.7
524,178 - CCNA_00509 0.35 +1.1
524,185 + CCNA_00509 0.36 -0.8
524,185 - CCNA_00509 0.36 -1.5
524,193 - CCNA_00509 0.37 -0.1
524,193 - CCNA_00509 0.37 -0.3
524,220 + CCNA_00509 0.43 +0.7
524,223 - CCNA_00509 0.43 -0.1
524,228 - CCNA_00509 0.44 -0.9
524,282 + CCNA_00509 0.54 +1.5
524,287 + CCNA_00509 0.55 +0.4
524,304 - CCNA_00509 0.58 +0.7
524,317 - CCNA_00509 0.61 -0.8
524,331 - CCNA_00509 0.63 -0.1
524,341 - CCNA_00509 0.65 +2.7
524,363 + CCNA_00509 0.69 +0.7
524,369 + CCNA_00509 0.70 +1.7
524,409 - CCNA_00509 0.78 +1.1
524,417 + CCNA_00509 0.79 -0.6
524,417 + CCNA_00509 0.79 -1.3
524,493 + +0.7
524,494 + +0.7
524,494 + -1.5
524,494 + +0.7
524,494 + +0.1
524,502 - -0.9
524,561 + -0.0
524,561 + -0.6
524,569 - +3.3
524,569 - +2.9
524,577 - +1.2
524,620 + CCNA_00510 0.14 +1.7
524,625 + CCNA_00510 0.15 -1.0
524,625 + CCNA_00510 0.15 +0.7
524,625 + CCNA_00510 0.15 -0.9
524,638 - CCNA_00510 0.17 +0.0
524,643 - CCNA_00510 0.18 -1.5
524,648 - CCNA_00510 0.18 -0.5
524,713 + CCNA_00510 0.28 -0.4
524,724 + CCNA_00510 0.30 -0.5
524,724 + CCNA_00510 0.30 +2.2
524,724 + CCNA_00510 0.30 -1.1
524,724 + CCNA_00510 0.30 +0.1
524,732 - CCNA_00510 0.31 +2.4
524,748 - CCNA_00510 0.33 -1.0
524,795 + CCNA_00510 0.40 -0.6
524,805 - CCNA_00510 0.42 +1.5
524,855 + CCNA_00510 0.49 +0.0
524,863 + CCNA_00510 0.50 -0.8
524,863 + CCNA_00510 0.50 -0.6
524,870 - CCNA_00510 0.51 +0.5
524,871 - CCNA_00510 0.51 +0.2
524,895 + CCNA_00510 0.55 +0.4
524,895 + CCNA_00510 0.55 -1.4
524,907 + CCNA_00510 0.57 +1.2
524,922 + CCNA_00510 0.59 +0.4

Or see this region's nucleotide sequence