Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00450

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00447 and CCNA_00448 are separated by 3 nucleotidesCCNA_00448 and CCNA_00449 are separated by 7 nucleotidesCCNA_00449 and CCNA_00450 overlap by 4 nucleotidesCCNA_00450 and CCNA_00451 are separated by 269 nucleotides CCNA_00447: CCNA_00447 - chemotaxis protein cheD, at 453,301 to 453,864 _00447 CCNA_00448: CCNA_00448 - cheU protein, at 453,868 to 454,251 _00448 CCNA_00449: CCNA_00449 - cheYIII, at 454,259 to 454,861 _00449 CCNA_00450: CCNA_00450 - cheE protein, at 454,858 to 455,328 _00450 CCNA_00451: CCNA_00451 - TonB-dependent receptor, at 455,598 to 458,639 _00451 Position (kb) 454 455 456Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.864 kb on + strandat 453.866 kb on - strandat 453.866 kb on - strandat 453.866 kb on - strandat 453.866 kb on - strandat 453.883 kb on + strandat 453.907 kb on + strand, within CCNA_00448at 453.918 kb on + strand, within CCNA_00448at 453.923 kb on - strand, within CCNA_00448at 453.979 kb on + strand, within CCNA_00448at 453.996 kb on + strand, within CCNA_00448at 453.996 kb on + strand, within CCNA_00448at 454.020 kb on - strand, within CCNA_00448at 454.046 kb on + strand, within CCNA_00448at 454.054 kb on - strand, within CCNA_00448at 454.056 kb on - strand, within CCNA_00448at 454.064 kb on + strand, within CCNA_00448at 454.121 kb on + strand, within CCNA_00448at 454.121 kb on + strand, within CCNA_00448at 454.121 kb on + strand, within CCNA_00448at 454.129 kb on + strand, within CCNA_00448at 454.129 kb on - strand, within CCNA_00448at 454.129 kb on - strand, within CCNA_00448at 454.163 kb on + strand, within CCNA_00448at 454.163 kb on + strand, within CCNA_00448at 454.163 kb on + strand, within CCNA_00448at 454.230 kb on - strandat 454.273 kb on + strandat 454.303 kb on + strandat 454.351 kb on + strand, within CCNA_00449at 454.355 kb on - strand, within CCNA_00449at 454.382 kb on + strand, within CCNA_00449at 454.390 kb on - strand, within CCNA_00449at 454.417 kb on + strand, within CCNA_00449at 454.417 kb on + strand, within CCNA_00449at 454.425 kb on - strand, within CCNA_00449at 454.429 kb on + strand, within CCNA_00449at 454.429 kb on + strand, within CCNA_00449at 454.437 kb on + strand, within CCNA_00449at 454.500 kb on - strand, within CCNA_00449at 454.509 kb on - strand, within CCNA_00449at 454.530 kb on + strand, within CCNA_00449at 454.561 kb on + strand, within CCNA_00449at 454.561 kb on + strand, within CCNA_00449at 454.569 kb on - strand, within CCNA_00449at 454.605 kb on + strand, within CCNA_00449at 454.613 kb on - strand, within CCNA_00449at 454.613 kb on - strand, within CCNA_00449at 454.662 kb on - strand, within CCNA_00449at 454.662 kb on - strand, within CCNA_00449at 454.673 kb on + strand, within CCNA_00449at 454.741 kb on + strand, within CCNA_00449at 454.751 kb on + strand, within CCNA_00449at 454.773 kb on - strand, within CCNA_00449at 454.800 kb on + strand, within CCNA_00449at 454.850 kb on + strandat 454.854 kb on + strandat 454.863 kb on - strandat 454.989 kb on - strand, within CCNA_00450at 454.997 kb on + strand, within CCNA_00450at 455.005 kb on - strand, within CCNA_00450at 455.071 kb on + strand, within CCNA_00450at 455.071 kb on + strand, within CCNA_00450at 455.076 kb on - strand, within CCNA_00450at 455.084 kb on - strand, within CCNA_00450at 455.123 kb on - strand, within CCNA_00450at 455.125 kb on + strand, within CCNA_00450at 455.125 kb on + strand, within CCNA_00450at 455.125 kb on + strand, within CCNA_00450at 455.126 kb on + strand, within CCNA_00450at 455.133 kb on - strand, within CCNA_00450at 455.171 kb on + strand, within CCNA_00450at 455.171 kb on + strand, within CCNA_00450at 455.174 kb on - strand, within CCNA_00450at 455.214 kb on + strand, within CCNA_00450at 455.214 kb on + strand, within CCNA_00450at 455.222 kb on + strand, within CCNA_00450at 455.230 kb on - strand, within CCNA_00450at 455.230 kb on - strand, within CCNA_00450at 455.240 kb on + strand, within CCNA_00450at 455.240 kb on + strand, within CCNA_00450at 455.240 kb on + strand, within CCNA_00450at 455.242 kb on + strand, within CCNA_00450at 455.258 kb on + strand, within CCNA_00450at 455.268 kb on + strand, within CCNA_00450at 455.276 kb on - strand, within CCNA_00450at 455.292 kb on + strandat 455.292 kb on + strandat 455.292 kb on + strandat 455.300 kb on - strandat 455.322 kb on + strandat 455.322 kb on + strandat 455.322 kb on + strandat 455.379 kb on + strandat 455.381 kb on + strandat 455.381 kb on + strandat 455.510 kb on - strandat 455.546 kb on + strandat 455.546 kb on + strandat 455.546 kb on + strandat 455.546 kb on + strandat 455.554 kb on - strandat 455.554 kb on - strandat 455.554 kb on - strandat 455.590 kb on - strandat 455.598 kb on - strandat 455.606 kb on + strandat 455.618 kb on - strandat 455.631 kb on + strandat 455.653 kb on + strandat 455.716 kb on + strandat 455.820 kb on + strandat 455.820 kb on + strandat 455.845 kb on + strandat 455.845 kb on + strandat 455.845 kb on + strandat 455.853 kb on - strandat 455.853 kb on - strandat 455.918 kb on - strand, within CCNA_00451at 455.938 kb on + strand, within CCNA_00451at 455.938 kb on + strand, within CCNA_00451at 455.938 kb on + strand, within CCNA_00451at 455.943 kb on + strand, within CCNA_00451at 455.946 kb on - strand, within CCNA_00451at 455.958 kb on + strand, within CCNA_00451at 455.976 kb on + strand, within CCNA_00451at 455.976 kb on - strand, within CCNA_00451at 456.052 kb on + strand, within CCNA_00451at 456.060 kb on + strand, within CCNA_00451at 456.062 kb on + strand, within CCNA_00451at 456.070 kb on - strand, within CCNA_00451at 456.078 kb on - strand, within CCNA_00451at 456.123 kb on - strand, within CCNA_00451at 456.167 kb on + strand, within CCNA_00451at 456.227 kb on + strand, within CCNA_00451at 456.227 kb on + strand, within CCNA_00451at 456.227 kb on + strand, within CCNA_00451at 456.227 kb on + strand, within CCNA_00451at 456.227 kb on + strand, within CCNA_00451at 456.228 kb on + strand, within CCNA_00451at 456.228 kb on + strand, within CCNA_00451at 456.235 kb on - strand, within CCNA_00451at 456.235 kb on - strand, within CCNA_00451at 456.236 kb on - strand, within CCNA_00451at 456.250 kb on + strand, within CCNA_00451at 456.287 kb on + strand, within CCNA_00451at 456.287 kb on + strand, within CCNA_00451

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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453,858 + +2.4
453,858 + +1.9
453,858 + +2.0
453,858 + +2.5
453,858 + +4.1
453,858 + +2.2
453,858 + +2.6
453,858 + +2.7
453,858 + +3.0
453,864 + +2.7
453,866 - -1.6
453,866 - +1.0
453,866 - -1.0
453,866 - +1.0
453,883 + +2.3
453,907 + CCNA_00448 0.10 +3.1
453,918 + CCNA_00448 0.13 +2.2
453,923 - CCNA_00448 0.14 -1.5
453,979 + CCNA_00448 0.29 +2.3
453,996 + CCNA_00448 0.33 +2.5
453,996 + CCNA_00448 0.33 +2.4
454,020 - CCNA_00448 0.40 +1.5
454,046 + CCNA_00448 0.46 +2.2
454,054 - CCNA_00448 0.48 -0.5
454,056 - CCNA_00448 0.49 +0.2
454,064 + CCNA_00448 0.51 +2.2
454,121 + CCNA_00448 0.66 +2.5
454,121 + CCNA_00448 0.66 +2.3
454,121 + CCNA_00448 0.66 +2.1
454,129 + CCNA_00448 0.68 +2.6
454,129 - CCNA_00448 0.68 +3.1
454,129 - CCNA_00448 0.68 +0.5
454,163 + CCNA_00448 0.77 +2.7
454,163 + CCNA_00448 0.77 +2.1
454,163 + CCNA_00448 0.77 +1.9
454,230 - +0.5
454,273 + +2.5
454,303 + +1.9
454,351 + CCNA_00449 0.15 +2.3
454,355 - CCNA_00449 0.16 -0.0
454,382 + CCNA_00449 0.20 +1.5
454,390 - CCNA_00449 0.22 -0.5
454,417 + CCNA_00449 0.26 +1.5
454,417 + CCNA_00449 0.26 +1.2
454,425 - CCNA_00449 0.28 -0.6
454,429 + CCNA_00449 0.28 +2.1
454,429 + CCNA_00449 0.28 +0.9
454,437 + CCNA_00449 0.30 -0.2
454,500 - CCNA_00449 0.40 -0.3
454,509 - CCNA_00449 0.41 -2.0
454,530 + CCNA_00449 0.45 +0.2
454,561 + CCNA_00449 0.50 +1.2
454,561 + CCNA_00449 0.50 +0.4
454,569 - CCNA_00449 0.51 +1.0
454,605 + CCNA_00449 0.57 +0.9
454,613 - CCNA_00449 0.59 +0.3
454,613 - CCNA_00449 0.59 -0.3
454,662 - CCNA_00449 0.67 +2.3
454,662 - CCNA_00449 0.67 -0.5
454,673 + CCNA_00449 0.69 +1.8
454,741 + CCNA_00449 0.80 -0.8
454,751 + CCNA_00449 0.82 +1.1
454,773 - CCNA_00449 0.85 -0.5
454,800 + CCNA_00449 0.90 +1.2
454,850 + +1.6
454,854 + +2.5
454,863 - -0.5
454,989 - CCNA_00450 0.28 -0.9
454,997 + CCNA_00450 0.30 +1.3
455,005 - CCNA_00450 0.31 -2.5
455,071 + CCNA_00450 0.45 +1.8
455,071 + CCNA_00450 0.45 +2.1
455,076 - CCNA_00450 0.46 -2.7
455,084 - CCNA_00450 0.48 -3.2
455,123 - CCNA_00450 0.56 -1.7
455,125 + CCNA_00450 0.57 +0.9
455,125 + CCNA_00450 0.57 +1.0
455,125 + CCNA_00450 0.57 +1.6
455,126 + CCNA_00450 0.57 +1.8
455,133 - CCNA_00450 0.58 +1.4
455,171 + CCNA_00450 0.66 +0.2
455,171 + CCNA_00450 0.66 +0.7
455,174 - CCNA_00450 0.67 -1.5
455,214 + CCNA_00450 0.76 -0.5
455,214 + CCNA_00450 0.76 +3.6
455,222 + CCNA_00450 0.77 +1.8
455,230 - CCNA_00450 0.79 -1.0
455,230 - CCNA_00450 0.79 -0.5
455,240 + CCNA_00450 0.81 +1.1
455,240 + CCNA_00450 0.81 +1.8
455,240 + CCNA_00450 0.81 +2.0
455,242 + CCNA_00450 0.82 +0.2
455,258 + CCNA_00450 0.85 +4.2
455,268 + CCNA_00450 0.87 +1.3
455,276 - CCNA_00450 0.89 +2.5
455,292 + +1.9
455,292 + +1.1
455,292 + +1.8
455,300 - +0.5
455,322 + +1.5
455,322 + +2.8
455,322 + +2.3
455,379 + +2.0
455,381 + +0.5
455,381 + +0.9
455,510 - -0.3
455,546 + -0.0
455,546 + -0.9
455,546 + -2.6
455,546 + +0.7
455,554 - -2.1
455,554 - +1.0
455,554 - -0.9
455,590 - -0.0
455,598 - -1.5
455,606 + -1.2
455,618 - -0.3
455,631 + -0.7
455,653 + -1.1
455,716 + -0.8
455,820 + +0.5
455,820 + +1.5
455,845 + +0.7
455,845 + -1.8
455,845 + -1.2
455,853 - -3.1
455,853 - +0.7
455,918 - CCNA_00451 0.11 -1.3
455,938 + CCNA_00451 0.11 +0.5
455,938 + CCNA_00451 0.11 +1.7
455,938 + CCNA_00451 0.11 +1.0
455,943 + CCNA_00451 0.11 -2.3
455,946 - CCNA_00451 0.11 -0.6
455,958 + CCNA_00451 0.12 -2.0
455,976 + CCNA_00451 0.12 -1.0
455,976 - CCNA_00451 0.12 -0.6
456,052 + CCNA_00451 0.15 +0.8
456,060 + CCNA_00451 0.15 -0.3
456,062 + CCNA_00451 0.15 +0.2
456,070 - CCNA_00451 0.16 -0.6
456,078 - CCNA_00451 0.16 +0.3
456,123 - CCNA_00451 0.17 -1.5
456,167 + CCNA_00451 0.19 -1.2
456,227 + CCNA_00451 0.21 -2.0
456,227 + CCNA_00451 0.21 -2.1
456,227 + CCNA_00451 0.21 -2.3
456,227 + CCNA_00451 0.21 +0.8
456,227 + CCNA_00451 0.21 +0.0
456,228 + CCNA_00451 0.21 -0.1
456,228 + CCNA_00451 0.21 -1.2
456,235 - CCNA_00451 0.21 -0.0
456,235 - CCNA_00451 0.21 +0.3
456,236 - CCNA_00451 0.21 -1.5
456,250 + CCNA_00451 0.21 -1.2
456,287 + CCNA_00451 0.23 +0.0
456,287 + CCNA_00451 0.23 +1.3

Or see this region's nucleotide sequence