Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00448

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00445 and CCNA_00446 are separated by 3 nucleotidesCCNA_00446 and CCNA_00447 overlap by 4 nucleotidesCCNA_00447 and CCNA_00448 are separated by 3 nucleotidesCCNA_00448 and CCNA_00449 are separated by 7 nucleotidesCCNA_00449 and CCNA_00450 overlap by 4 nucleotides CCNA_00445: CCNA_00445 - receiver domain-glutamate methylesterase cheBI, at 451,877 to 452,911 _00445 CCNA_00446: CCNA_00446 - chemotaxis receiver domain protein cheYII, at 452,915 to 453,304 _00446 CCNA_00447: CCNA_00447 - chemotaxis protein cheD, at 453,301 to 453,864 _00447 CCNA_00448: CCNA_00448 - cheU protein, at 453,868 to 454,251 _00448 CCNA_00449: CCNA_00449 - cheYIII, at 454,259 to 454,861 _00449 CCNA_00450: CCNA_00450 - cheE protein, at 454,858 to 455,328 _00450 Position (kb) 453 454 455Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 452.888 kb on + strandat 452.913 kb on - strandat 452.917 kb on + strandat 452.945 kb on - strandat 452.967 kb on - strand, within CCNA_00446at 453.013 kb on + strand, within CCNA_00446at 453.013 kb on + strand, within CCNA_00446at 453.023 kb on - strand, within CCNA_00446at 453.044 kb on + strand, within CCNA_00446at 453.052 kb on - strand, within CCNA_00446at 453.058 kb on + strand, within CCNA_00446at 453.060 kb on - strand, within CCNA_00446at 453.111 kb on + strand, within CCNA_00446at 453.116 kb on + strand, within CCNA_00446at 453.151 kb on + strand, within CCNA_00446at 453.195 kb on + strand, within CCNA_00446at 453.212 kb on + strand, within CCNA_00446at 453.212 kb on + strand, within CCNA_00446at 453.220 kb on - strand, within CCNA_00446at 453.232 kb on + strand, within CCNA_00446at 453.237 kb on + strand, within CCNA_00446at 453.244 kb on + strand, within CCNA_00446at 453.245 kb on - strand, within CCNA_00446at 453.247 kb on - strand, within CCNA_00446at 453.252 kb on - strand, within CCNA_00446at 453.252 kb on - strand, within CCNA_00446at 453.254 kb on + strand, within CCNA_00446at 453.262 kb on - strand, within CCNA_00446at 453.293 kb on - strandat 453.295 kb on + strandat 453.306 kb on - strandat 453.311 kb on - strandat 453.337 kb on + strandat 453.337 kb on + strandat 453.337 kb on + strandat 453.337 kb on + strandat 453.343 kb on + strandat 453.357 kb on + strandat 453.399 kb on - strand, within CCNA_00447at 453.413 kb on - strand, within CCNA_00447at 453.439 kb on + strand, within CCNA_00447at 453.439 kb on + strand, within CCNA_00447at 453.439 kb on + strand, within CCNA_00447at 453.463 kb on + strand, within CCNA_00447at 453.512 kb on - strand, within CCNA_00447at 453.516 kb on + strand, within CCNA_00447at 453.591 kb on + strand, within CCNA_00447at 453.592 kb on + strand, within CCNA_00447at 453.608 kb on + strand, within CCNA_00447at 453.618 kb on - strand, within CCNA_00447at 453.630 kb on + strand, within CCNA_00447at 453.637 kb on + strand, within CCNA_00447at 453.679 kb on + strand, within CCNA_00447at 453.702 kb on + strand, within CCNA_00447at 453.709 kb on - strand, within CCNA_00447at 453.719 kb on + strand, within CCNA_00447at 453.738 kb on + strand, within CCNA_00447at 453.738 kb on + strand, within CCNA_00447at 453.807 kb on + strand, within CCNA_00447at 453.809 kb on - strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.858 kb on + strandat 453.864 kb on + strandat 453.866 kb on - strandat 453.866 kb on - strandat 453.866 kb on - strandat 453.866 kb on - strandat 453.883 kb on + strandat 453.907 kb on + strand, within CCNA_00448at 453.918 kb on + strand, within CCNA_00448at 453.923 kb on - strand, within CCNA_00448at 453.979 kb on + strand, within CCNA_00448at 453.996 kb on + strand, within CCNA_00448at 453.996 kb on + strand, within CCNA_00448at 454.020 kb on - strand, within CCNA_00448at 454.046 kb on + strand, within CCNA_00448at 454.054 kb on - strand, within CCNA_00448at 454.056 kb on - strand, within CCNA_00448at 454.064 kb on + strand, within CCNA_00448at 454.121 kb on + strand, within CCNA_00448at 454.121 kb on + strand, within CCNA_00448at 454.121 kb on + strand, within CCNA_00448at 454.129 kb on + strand, within CCNA_00448at 454.129 kb on - strand, within CCNA_00448at 454.129 kb on - strand, within CCNA_00448at 454.163 kb on + strand, within CCNA_00448at 454.163 kb on + strand, within CCNA_00448at 454.163 kb on + strand, within CCNA_00448at 454.230 kb on - strandat 454.273 kb on + strandat 454.303 kb on + strandat 454.351 kb on + strand, within CCNA_00449at 454.355 kb on - strand, within CCNA_00449at 454.382 kb on + strand, within CCNA_00449at 454.390 kb on - strand, within CCNA_00449at 454.417 kb on + strand, within CCNA_00449at 454.417 kb on + strand, within CCNA_00449at 454.425 kb on - strand, within CCNA_00449at 454.429 kb on + strand, within CCNA_00449at 454.429 kb on + strand, within CCNA_00449at 454.437 kb on + strand, within CCNA_00449at 454.500 kb on - strand, within CCNA_00449at 454.509 kb on - strand, within CCNA_00449at 454.530 kb on + strand, within CCNA_00449at 454.561 kb on + strand, within CCNA_00449at 454.561 kb on + strand, within CCNA_00449at 454.569 kb on - strand, within CCNA_00449at 454.605 kb on + strand, within CCNA_00449at 454.613 kb on - strand, within CCNA_00449at 454.613 kb on - strand, within CCNA_00449at 454.662 kb on - strand, within CCNA_00449at 454.662 kb on - strand, within CCNA_00449at 454.673 kb on + strand, within CCNA_00449at 454.741 kb on + strand, within CCNA_00449at 454.751 kb on + strand, within CCNA_00449at 454.773 kb on - strand, within CCNA_00449at 454.800 kb on + strand, within CCNA_00449at 454.850 kb on + strandat 454.854 kb on + strandat 454.863 kb on - strandat 454.989 kb on - strand, within CCNA_00450at 454.997 kb on + strand, within CCNA_00450at 455.005 kb on - strand, within CCNA_00450at 455.071 kb on + strand, within CCNA_00450at 455.071 kb on + strand, within CCNA_00450at 455.076 kb on - strand, within CCNA_00450at 455.084 kb on - strand, within CCNA_00450at 455.123 kb on - strand, within CCNA_00450at 455.125 kb on + strand, within CCNA_00450at 455.125 kb on + strand, within CCNA_00450at 455.125 kb on + strand, within CCNA_00450at 455.126 kb on + strand, within CCNA_00450at 455.133 kb on - strand, within CCNA_00450at 455.171 kb on + strand, within CCNA_00450at 455.171 kb on + strand, within CCNA_00450at 455.174 kb on - strand, within CCNA_00450at 455.214 kb on + strand, within CCNA_00450at 455.214 kb on + strand, within CCNA_00450at 455.222 kb on + strand, within CCNA_00450at 455.230 kb on - strand, within CCNA_00450at 455.230 kb on - strand, within CCNA_00450at 455.240 kb on + strand, within CCNA_00450at 455.240 kb on + strand, within CCNA_00450at 455.240 kb on + strand, within CCNA_00450at 455.242 kb on + strand, within CCNA_00450

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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452,888 + +3.2
452,913 - +1.0
452,917 + +3.2
452,945 - +0.2
452,967 - CCNA_00446 0.13 +0.2
453,013 + CCNA_00446 0.25 +2.9
453,013 + CCNA_00446 0.25 +2.7
453,023 - CCNA_00446 0.28 -0.5
453,044 + CCNA_00446 0.33 +1.9
453,052 - CCNA_00446 0.35 -0.9
453,058 + CCNA_00446 0.37 +2.7
453,060 - CCNA_00446 0.37 -0.6
453,111 + CCNA_00446 0.50 +2.5
453,116 + CCNA_00446 0.52 +2.1
453,151 + CCNA_00446 0.61 +2.3
453,195 + CCNA_00446 0.72 +1.9
453,212 + CCNA_00446 0.76 +2.8
453,212 + CCNA_00446 0.76 +2.6
453,220 - CCNA_00446 0.78 -1.4
453,232 + CCNA_00446 0.81 +1.9
453,237 + CCNA_00446 0.83 +2.2
453,244 + CCNA_00446 0.84 +3.0
453,245 - CCNA_00446 0.85 -2.0
453,247 - CCNA_00446 0.85 -0.8
453,252 - CCNA_00446 0.86 -1.4
453,252 - CCNA_00446 0.86 -0.5
453,254 + CCNA_00446 0.87 +2.2
453,262 - CCNA_00446 0.89 -1.6
453,293 - +0.2
453,295 + +2.4
453,306 - -1.2
453,311 - +0.1
453,337 + +2.5
453,337 + +2.5
453,337 + +2.1
453,337 + +2.9
453,343 + +2.9
453,357 + +2.5
453,399 - CCNA_00447 0.17 -0.5
453,413 - CCNA_00447 0.20 -1.4
453,439 + CCNA_00447 0.24 +3.2
453,439 + CCNA_00447 0.24 +2.1
453,439 + CCNA_00447 0.24 +2.7
453,463 + CCNA_00447 0.29 +2.4
453,512 - CCNA_00447 0.37 -0.5
453,516 + CCNA_00447 0.38 +1.4
453,591 + CCNA_00447 0.51 +2.6
453,592 + CCNA_00447 0.52 +3.0
453,608 + CCNA_00447 0.54 +2.4
453,618 - CCNA_00447 0.56 -0.0
453,630 + CCNA_00447 0.58 +1.5
453,637 + CCNA_00447 0.60 +2.4
453,679 + CCNA_00447 0.67 +6.1
453,702 + CCNA_00447 0.71 +2.8
453,709 - CCNA_00447 0.72 +1.5
453,719 + CCNA_00447 0.74 +2.1
453,738 + CCNA_00447 0.77 +3.2
453,738 + CCNA_00447 0.77 +3.2
453,807 + CCNA_00447 0.90 +2.5
453,809 - -0.5
453,858 + +2.4
453,858 + +1.9
453,858 + +2.0
453,858 + +2.5
453,858 + +4.1
453,858 + +2.2
453,858 + +2.6
453,858 + +2.7
453,858 + +3.0
453,864 + +2.7
453,866 - -1.6
453,866 - +1.0
453,866 - -1.0
453,866 - +1.0
453,883 + +2.3
453,907 + CCNA_00448 0.10 +3.1
453,918 + CCNA_00448 0.13 +2.2
453,923 - CCNA_00448 0.14 -1.5
453,979 + CCNA_00448 0.29 +2.3
453,996 + CCNA_00448 0.33 +2.5
453,996 + CCNA_00448 0.33 +2.4
454,020 - CCNA_00448 0.40 +1.5
454,046 + CCNA_00448 0.46 +2.2
454,054 - CCNA_00448 0.48 -0.5
454,056 - CCNA_00448 0.49 +0.2
454,064 + CCNA_00448 0.51 +2.2
454,121 + CCNA_00448 0.66 +2.5
454,121 + CCNA_00448 0.66 +2.3
454,121 + CCNA_00448 0.66 +2.1
454,129 + CCNA_00448 0.68 +2.6
454,129 - CCNA_00448 0.68 +3.1
454,129 - CCNA_00448 0.68 +0.5
454,163 + CCNA_00448 0.77 +2.7
454,163 + CCNA_00448 0.77 +2.1
454,163 + CCNA_00448 0.77 +1.9
454,230 - +0.5
454,273 + +2.5
454,303 + +1.9
454,351 + CCNA_00449 0.15 +2.3
454,355 - CCNA_00449 0.16 -0.0
454,382 + CCNA_00449 0.20 +1.5
454,390 - CCNA_00449 0.22 -0.5
454,417 + CCNA_00449 0.26 +1.5
454,417 + CCNA_00449 0.26 +1.2
454,425 - CCNA_00449 0.28 -0.6
454,429 + CCNA_00449 0.28 +2.1
454,429 + CCNA_00449 0.28 +0.9
454,437 + CCNA_00449 0.30 -0.2
454,500 - CCNA_00449 0.40 -0.3
454,509 - CCNA_00449 0.41 -2.0
454,530 + CCNA_00449 0.45 +0.2
454,561 + CCNA_00449 0.50 +1.2
454,561 + CCNA_00449 0.50 +0.4
454,569 - CCNA_00449 0.51 +1.0
454,605 + CCNA_00449 0.57 +0.9
454,613 - CCNA_00449 0.59 +0.3
454,613 - CCNA_00449 0.59 -0.3
454,662 - CCNA_00449 0.67 +2.3
454,662 - CCNA_00449 0.67 -0.5
454,673 + CCNA_00449 0.69 +1.8
454,741 + CCNA_00449 0.80 -0.8
454,751 + CCNA_00449 0.82 +1.1
454,773 - CCNA_00449 0.85 -0.5
454,800 + CCNA_00449 0.90 +1.2
454,850 + +1.6
454,854 + +2.5
454,863 - -0.5
454,989 - CCNA_00450 0.28 -0.9
454,997 + CCNA_00450 0.30 +1.3
455,005 - CCNA_00450 0.31 -2.5
455,071 + CCNA_00450 0.45 +1.8
455,071 + CCNA_00450 0.45 +2.1
455,076 - CCNA_00450 0.46 -2.7
455,084 - CCNA_00450 0.48 -3.2
455,123 - CCNA_00450 0.56 -1.7
455,125 + CCNA_00450 0.57 +0.9
455,125 + CCNA_00450 0.57 +1.0
455,125 + CCNA_00450 0.57 +1.6
455,126 + CCNA_00450 0.57 +1.8
455,133 - CCNA_00450 0.58 +1.4
455,171 + CCNA_00450 0.66 +0.2
455,171 + CCNA_00450 0.66 +0.7
455,174 - CCNA_00450 0.67 -1.5
455,214 + CCNA_00450 0.76 -0.5
455,214 + CCNA_00450 0.76 +3.6
455,222 + CCNA_00450 0.77 +1.8
455,230 - CCNA_00450 0.79 -1.0
455,230 - CCNA_00450 0.79 -0.5
455,240 + CCNA_00450 0.81 +1.1
455,240 + CCNA_00450 0.81 +1.8
455,240 + CCNA_00450 0.81 +2.0
455,242 + CCNA_00450 0.82 +0.2

Or see this region's nucleotide sequence