Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00343 and CCNA_00344 are separated by 59 nucleotides CCNA_00344 and CCNA_00345 are separated by 7 nucleotides
CCNA_00343: CCNA_00343 - dihydrolipoamide succinyltransferase, at 357,720 to 358,928
_00343
CCNA_00344: CCNA_00344 - hypothetical protein, at 358,988 to 359,335
_00344
CCNA_00345: CCNA_00345 - alpha/beta family hydrolase, at 359,343 to 360,176
_00345
Position (kb) 358
359
360 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 358.933 kb on + strand at 358.980 kb on + strand at 358.988 kb on - strand at 358.990 kb on + strand at 358.990 kb on + strand at 358.990 kb on + strand at 358.990 kb on + strand at 358.990 kb on + strand at 358.990 kb on + strand at 358.997 kb on + strand at 358.998 kb on - strand at 358.998 kb on - strand at 358.998 kb on - strand at 358.998 kb on - strand at 358.998 kb on - strand at 359.001 kb on + strand at 359.015 kb on - strand at 359.042 kb on + strand, within CCNA_00344 at 359.049 kb on - strand, within CCNA_00344 at 359.050 kb on - strand, within CCNA_00344 at 359.050 kb on - strand, within CCNA_00344 at 359.050 kb on - strand, within CCNA_00344 at 359.050 kb on - strand, within CCNA_00344 at 359.099 kb on - strand, within CCNA_00344 at 359.127 kb on - strand, within CCNA_00344 at 359.133 kb on + strand, within CCNA_00344 at 359.163 kb on - strand, within CCNA_00344 at 359.167 kb on + strand, within CCNA_00344 at 359.175 kb on - strand, within CCNA_00344 at 359.175 kb on - strand, within CCNA_00344 at 359.192 kb on + strand, within CCNA_00344 at 359.205 kb on - strand, within CCNA_00344 at 359.277 kb on - strand, within CCNA_00344 at 359.324 kb on + strand at 359.330 kb on + strand at 359.361 kb on + strand at 359.388 kb on - strand at 359.407 kb on + strand at 359.407 kb on + strand at 359.415 kb on - strand at 359.434 kb on + strand, within CCNA_00345 at 359.445 kb on + strand, within CCNA_00345 at 359.475 kb on - strand, within CCNA_00345 at 359.487 kb on - strand, within CCNA_00345 at 359.497 kb on + strand, within CCNA_00345 at 359.499 kb on + strand, within CCNA_00345 at 359.505 kb on - strand, within CCNA_00345 at 359.507 kb on - strand, within CCNA_00345 at 359.530 kb on + strand, within CCNA_00345 at 359.538 kb on - strand, within CCNA_00345 at 359.542 kb on - strand, within CCNA_00345 at 359.558 kb on - strand, within CCNA_00345 at 359.608 kb on + strand, within CCNA_00345 at 359.612 kb on + strand at 359.618 kb on + strand, within CCNA_00345 at 359.618 kb on + strand, within CCNA_00345 at 359.626 kb on - strand, within CCNA_00345 at 359.689 kb on - strand, within CCNA_00345 at 359.697 kb on + strand, within CCNA_00345 at 359.718 kb on + strand, within CCNA_00345 at 359.741 kb on - strand, within CCNA_00345 at 359.752 kb on + strand, within CCNA_00345 at 359.754 kb on - strand, within CCNA_00345 at 359.760 kb on - strand, within CCNA_00345 at 359.760 kb on - strand, within CCNA_00345 at 359.760 kb on - strand, within CCNA_00345 at 359.760 kb on - strand, within CCNA_00345 at 359.796 kb on + strand, within CCNA_00345 at 359.801 kb on + strand, within CCNA_00345 at 359.804 kb on - strand, within CCNA_00345 at 359.820 kb on + strand, within CCNA_00345 at 359.827 kb on - strand, within CCNA_00345 at 359.833 kb on + strand, within CCNA_00345 at 359.833 kb on + strand, within CCNA_00345 at 359.841 kb on - strand, within CCNA_00345 at 359.841 kb on - strand, within CCNA_00345 at 359.841 kb on - strand, within CCNA_00345 at 359.841 kb on - strand, within CCNA_00345 at 359.841 kb on - strand, within CCNA_00345 at 359.844 kb on + strand, within CCNA_00345 at 359.877 kb on - strand, within CCNA_00345 at 359.901 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.902 kb on + strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.910 kb on - strand, within CCNA_00345 at 359.922 kb on - strand, within CCNA_00345 at 359.981 kb on + strand at 359.981 kb on + strand, within CCNA_00345 at 359.984 kb on - strand, within CCNA_00345 at 359.984 kb on - strand, within CCNA_00345 at 359.991 kb on + strand, within CCNA_00345 at 359.999 kb on - strand, within CCNA_00345 at 359.999 kb on - strand, within CCNA_00345 at 359.999 kb on - strand, within CCNA_00345 at 360.037 kb on + strand, within CCNA_00345 at 360.063 kb on + strand, within CCNA_00345 at 360.063 kb on - strand, within CCNA_00345 at 360.063 kb on - strand, within CCNA_00345 at 360.071 kb on - strand, within CCNA_00345 at 360.123 kb on - strand at 360.148 kb on + strand at 360.161 kb on - strand at 360.161 kb on - strand at 360.161 kb on - strand at 360.161 kb on - strand at 360.166 kb on + strand at 360.166 kb on - strand at 360.178 kb on + strand at 360.178 kb on + strand at 360.185 kb on + strand at 360.198 kb on - strand at 360.206 kb on - strand at 360.207 kb on + strand at 360.207 kb on + strand at 360.215 kb on - strand at 360.226 kb on + strand at 360.231 kb on + strand at 360.239 kb on - strand at 360.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 358,933 + +3.3 358,980 + +1.6 358,988 - +1.2 358,990 + +1.8 358,990 + +0.9 358,990 + +0.8 358,990 + +2.7 358,990 + +1.2 358,990 + +1.8 358,997 + +3.4 358,998 - +1.7 358,998 - -1.0 358,998 - -2.5 358,998 - -0.5 358,998 - +0.8 359,001 + +2.2 359,015 - -1.0 359,042 + CCNA_00344 0.16 +1.7 359,049 - CCNA_00344 0.18 -2.3 359,050 - CCNA_00344 0.18 -1.2 359,050 - CCNA_00344 0.18 +0.8 359,050 - CCNA_00344 0.18 -0.6 359,050 - CCNA_00344 0.18 -0.7 359,099 - CCNA_00344 0.32 +1.4 359,127 - CCNA_00344 0.40 -1.8 359,133 + CCNA_00344 0.42 +0.3 359,163 - CCNA_00344 0.50 -1.3 359,167 + CCNA_00344 0.51 +1.0 359,175 - CCNA_00344 0.54 +0.4 359,175 - CCNA_00344 0.54 -0.2 359,192 + CCNA_00344 0.59 +1.0 359,205 - CCNA_00344 0.62 +1.0 359,277 - CCNA_00344 0.83 +0.8 359,324 + +2.1 359,330 + +2.0 359,361 + +1.2 359,388 - -1.6 359,407 + +0.4 359,407 + +1.0 359,415 - +1.1 359,434 + CCNA_00345 0.11 +0.1 359,445 + CCNA_00345 0.12 +0.3 359,475 - CCNA_00345 0.16 -1.7 359,487 - CCNA_00345 0.17 +0.7 359,497 + CCNA_00345 0.18 +1.6 359,499 + CCNA_00345 0.19 +1.2 359,505 - CCNA_00345 0.19 -0.8 359,507 - CCNA_00345 0.20 +2.1 359,530 + CCNA_00345 0.22 +0.1 359,538 - CCNA_00345 0.23 +1.4 359,542 - CCNA_00345 0.24 -1.5 359,558 - CCNA_00345 0.26 -3.3 359,608 + CCNA_00345 0.32 -0.8 359,612 + +3.4 359,618 + CCNA_00345 0.33 -1.4 359,618 + CCNA_00345 0.33 -2.0 359,626 - CCNA_00345 0.34 -0.6 359,689 - CCNA_00345 0.41 -0.3 359,697 + CCNA_00345 0.42 -2.0 359,718 + CCNA_00345 0.45 +1.8 359,741 - CCNA_00345 0.48 -1.6 359,752 + CCNA_00345 0.49 +0.1 359,754 - CCNA_00345 0.49 +0.6 359,760 - CCNA_00345 0.50 -0.4 359,760 - CCNA_00345 0.50 -0.5 359,760 - CCNA_00345 0.50 -1.0 359,760 - CCNA_00345 0.50 -2.0 359,796 + CCNA_00345 0.54 +1.0 359,801 + CCNA_00345 0.55 +0.4 359,804 - CCNA_00345 0.55 +1.6 359,820 + CCNA_00345 0.57 -2.0 359,827 - CCNA_00345 0.58 -1.8 359,833 + CCNA_00345 0.59 -0.9 359,833 + CCNA_00345 0.59 +1.9 359,841 - CCNA_00345 0.60 +0.4 359,841 - CCNA_00345 0.60 +0.4 359,841 - CCNA_00345 0.60 -0.5 359,841 - CCNA_00345 0.60 +1.4 359,841 - CCNA_00345 0.60 -1.0 359,844 + CCNA_00345 0.60 -1.6 359,877 - CCNA_00345 0.64 +1.8 359,901 + CCNA_00345 0.67 +0.4 359,902 + CCNA_00345 0.67 -1.8 359,902 + CCNA_00345 0.67 +1.3 359,902 + CCNA_00345 0.67 -0.4 359,902 + CCNA_00345 0.67 -0.6 359,902 + CCNA_00345 0.67 -1.0 359,902 + CCNA_00345 0.67 -1.4 359,902 + CCNA_00345 0.67 +0.4 359,902 + CCNA_00345 0.67 -0.2 359,902 + CCNA_00345 0.67 +0.4 359,902 + CCNA_00345 0.67 -0.6 359,902 + CCNA_00345 0.67 -0.7 359,910 - CCNA_00345 0.68 -2.0 359,910 - CCNA_00345 0.68 -1.0 359,910 - CCNA_00345 0.68 -1.0 359,910 - CCNA_00345 0.68 -1.8 359,910 - CCNA_00345 0.68 +0.4 359,910 - CCNA_00345 0.68 -0.0 359,910 - CCNA_00345 0.68 +1.1 359,910 - CCNA_00345 0.68 -1.0 359,910 - CCNA_00345 0.68 -1.0 359,910 - CCNA_00345 0.68 -0.2 359,910 - CCNA_00345 0.68 -1.0 359,910 - CCNA_00345 0.68 -1.5 359,922 - CCNA_00345 0.69 -2.2 359,981 + +3.4 359,981 + CCNA_00345 0.76 -0.2 359,984 - CCNA_00345 0.77 +0.8 359,984 - CCNA_00345 0.77 -1.0 359,991 + CCNA_00345 0.78 +1.3 359,999 - CCNA_00345 0.79 +2.4 359,999 - CCNA_00345 0.79 -0.3 359,999 - CCNA_00345 0.79 +0.4 360,037 + CCNA_00345 0.83 -2.2 360,063 + CCNA_00345 0.86 +0.0 360,063 - CCNA_00345 0.86 +1.3 360,063 - CCNA_00345 0.86 +0.1 360,071 - CCNA_00345 0.87 -1.3 360,123 - +0.4 360,148 + -0.0 360,161 - +0.4 360,161 - +2.0 360,161 - -2.0 360,161 - -0.4 360,166 + -0.2 360,166 - +0.8 360,178 + -2.0 360,178 + +2.4 360,185 + +0.1 360,198 - -0.1 360,206 - -2.2 360,207 + -1.5 360,207 + +2.4 360,215 - +0.1 360,226 + -1.2 360,231 + +1.4 360,239 - -2.2 360,255 - -0.8
Or see this region's nucleotide sequence