Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00296

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00294 and CCNA_00295 are separated by 25 nucleotidesCCNA_00295 and CCNA_00296 are separated by 21 nucleotidesCCNA_00296 and CCNA_00297 overlap by 28 nucleotidesCCNA_00297 and CCNA_00298 overlap by 4 nucleotides CCNA_00294: CCNA_00294 - phosphate transport ATP-binding protein pstB, at 308,810 to 309,634 _00294 CCNA_00295: CCNA_00295 - phosphate transport system protein phoU, at 309,660 to 310,352 _00295 CCNA_00296: CCNA_00296 - phosphate regulon response regulator PhoB, at 310,374 to 311,066 _00296 CCNA_00297: CCNA_00297 - two-component response regulator, at 311,039 to 311,896 _00297 CCNA_00298: CCNA_00298 - hypothetical protein, at 311,893 to 312,666 _00298 Position (kb) 310 311 312Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 309.555 kb on + strandat 309.633 kb on + strandat 310.315 kb on + strandat 310.324 kb on + strandat 310.347 kb on + strandat 310.353 kb on + strandat 310.401 kb on + strandat 310.410 kb on + strandat 310.418 kb on - strandat 310.418 kb on - strandat 310.423 kb on - strandat 310.454 kb on - strand, within CCNA_00296at 310.484 kb on - strand, within CCNA_00296at 310.493 kb on - strand, within CCNA_00296at 310.549 kb on - strand, within CCNA_00296at 310.549 kb on - strand, within CCNA_00296at 310.602 kb on + strand, within CCNA_00296at 310.602 kb on + strand, within CCNA_00296at 310.723 kb on + strand, within CCNA_00296at 310.723 kb on + strand, within CCNA_00296at 310.731 kb on - strand, within CCNA_00296at 310.731 kb on - strand, within CCNA_00296at 310.757 kb on - strand, within CCNA_00296at 310.834 kb on + strand, within CCNA_00296at 310.840 kb on + strand, within CCNA_00296at 310.870 kb on - strand, within CCNA_00296at 310.892 kb on + strand, within CCNA_00296at 310.919 kb on + strand, within CCNA_00296at 310.994 kb on - strand, within CCNA_00296at 311.023 kb on - strandat 311.048 kb on + strandat 311.069 kb on + strandat 311.069 kb on + strandat 311.069 kb on + strandat 311.076 kb on - strandat 311.077 kb on - strandat 311.078 kb on + strandat 311.099 kb on - strandat 311.113 kb on + strandat 311.113 kb on + strandat 311.113 kb on + strandat 311.121 kb on - strandat 311.121 kb on - strandat 311.124 kb on + strandat 311.124 kb on + strandat 311.124 kb on + strandat 311.139 kb on + strand, within CCNA_00297at 311.144 kb on + strand, within CCNA_00297at 311.150 kb on + strand, within CCNA_00297at 311.152 kb on - strand, within CCNA_00297at 311.152 kb on - strand, within CCNA_00297at 311.172 kb on + strand, within CCNA_00297at 311.186 kb on - strand, within CCNA_00297at 311.188 kb on + strand, within CCNA_00297at 311.193 kb on + strand, within CCNA_00297at 311.193 kb on + strand, within CCNA_00297at 311.201 kb on - strand, within CCNA_00297at 311.207 kb on + strand, within CCNA_00297at 311.207 kb on + strand, within CCNA_00297at 311.207 kb on + strand, within CCNA_00297at 311.210 kb on - strand, within CCNA_00297at 311.215 kb on - strand, within CCNA_00297at 311.245 kb on + strand, within CCNA_00297at 311.265 kb on - strand, within CCNA_00297at 311.265 kb on - strand, within CCNA_00297at 311.265 kb on - strand, within CCNA_00297at 311.267 kb on + strand, within CCNA_00297at 311.267 kb on + strand, within CCNA_00297at 311.267 kb on + strand, within CCNA_00297at 311.275 kb on - strand, within CCNA_00297at 311.275 kb on - strand, within CCNA_00297at 311.275 kb on - strand, within CCNA_00297at 311.275 kb on - strand, within CCNA_00297at 311.275 kb on - strand, within CCNA_00297at 311.282 kb on + strand, within CCNA_00297at 311.282 kb on + strand, within CCNA_00297at 311.290 kb on - strand, within CCNA_00297at 311.290 kb on - strand, within CCNA_00297at 311.329 kb on - strand, within CCNA_00297at 311.329 kb on - strand, within CCNA_00297at 311.349 kb on + strand, within CCNA_00297at 311.360 kb on - strand, within CCNA_00297at 311.366 kb on + strand, within CCNA_00297at 311.381 kb on + strand, within CCNA_00297at 311.408 kb on + strand, within CCNA_00297at 311.408 kb on + strand, within CCNA_00297at 311.416 kb on - strand, within CCNA_00297at 311.416 kb on - strand, within CCNA_00297at 311.416 kb on - strand, within CCNA_00297at 311.416 kb on - strand, within CCNA_00297at 311.445 kb on + strand, within CCNA_00297at 311.453 kb on - strand, within CCNA_00297at 311.494 kb on - strand, within CCNA_00297at 311.577 kb on - strand, within CCNA_00297at 311.577 kb on - strand, within CCNA_00297at 311.594 kb on + strand, within CCNA_00297at 311.602 kb on - strand, within CCNA_00297at 311.602 kb on - strand, within CCNA_00297at 311.602 kb on - strand, within CCNA_00297at 311.632 kb on - strand, within CCNA_00297at 311.670 kb on + strand, within CCNA_00297at 311.690 kb on - strand, within CCNA_00297at 311.690 kb on - strand, within CCNA_00297at 311.690 kb on - strand, within CCNA_00297at 311.706 kb on - strand, within CCNA_00297at 311.741 kb on + strand, within CCNA_00297at 311.741 kb on + strand, within CCNA_00297at 311.751 kb on + strand, within CCNA_00297at 311.754 kb on - strand, within CCNA_00297at 311.765 kb on - strand, within CCNA_00297at 311.772 kb on + strand, within CCNA_00297at 311.775 kb on - strand, within CCNA_00297at 311.846 kb on + strandat 311.846 kb on + strandat 311.846 kb on + strandat 311.846 kb on + strandat 311.854 kb on - strandat 311.889 kb on + strandat 311.897 kb on - strandat 311.897 kb on - strandat 311.900 kb on + strandat 311.936 kb on + strandat 311.954 kb on + strandat 311.954 kb on + strandat 311.962 kb on + strandat 311.962 kb on + strandat 311.962 kb on - strandat 311.967 kb on + strandat 311.980 kb on - strand, within CCNA_00298at 312.004 kb on + strand, within CCNA_00298at 312.033 kb on + strand, within CCNA_00298at 312.041 kb on - strand, within CCNA_00298

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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309,555 + +4.1
309,633 + +1.9
310,315 + +0.3
310,324 + -1.6
310,347 + +1.8
310,353 + -0.9
310,401 + +2.5
310,410 + +2.9
310,418 - +2.3
310,418 - +2.7
310,423 - +2.3
310,454 - CCNA_00296 0.12 +3.9
310,484 - CCNA_00296 0.16 +2.4
310,493 - CCNA_00296 0.17 +2.1
310,549 - CCNA_00296 0.25 +3.2
310,549 - CCNA_00296 0.25 +1.3
310,602 + CCNA_00296 0.33 +2.2
310,602 + CCNA_00296 0.33 +3.7
310,723 + CCNA_00296 0.50 +2.3
310,723 + CCNA_00296 0.50 +2.3
310,731 - CCNA_00296 0.52 +3.6
310,731 - CCNA_00296 0.52 +2.9
310,757 - CCNA_00296 0.55 +2.0
310,834 + CCNA_00296 0.66 +2.7
310,840 + CCNA_00296 0.67 +1.7
310,870 - CCNA_00296 0.72 +2.8
310,892 + CCNA_00296 0.75 +2.3
310,919 + CCNA_00296 0.79 +2.4
310,994 - CCNA_00296 0.89 +1.4
311,023 - +2.5
311,048 + +1.2
311,069 + +1.2
311,069 + +1.4
311,069 + +0.3
311,076 - +0.3
311,077 - -1.5
311,078 + -1.4
311,099 - -2.5
311,113 + +0.2
311,113 + +1.4
311,113 + -1.7
311,121 - -0.7
311,121 - -2.5
311,124 + -0.0
311,124 + +0.5
311,124 + +1.1
311,139 + CCNA_00297 0.12 +1.7
311,144 + CCNA_00297 0.12 -0.6
311,150 + CCNA_00297 0.13 +0.5
311,152 - CCNA_00297 0.13 -3.2
311,152 - CCNA_00297 0.13 +0.0
311,172 + CCNA_00297 0.16 -1.4
311,186 - CCNA_00297 0.17 -1.0
311,188 + CCNA_00297 0.17 +0.3
311,193 + CCNA_00297 0.18 +0.4
311,193 + CCNA_00297 0.18 -0.8
311,201 - CCNA_00297 0.19 +0.2
311,207 + CCNA_00297 0.20 +1.9
311,207 + CCNA_00297 0.20 +0.1
311,207 + CCNA_00297 0.20 -0.4
311,210 - CCNA_00297 0.20 -0.5
311,215 - CCNA_00297 0.21 +0.2
311,245 + CCNA_00297 0.24 +1.9
311,265 - CCNA_00297 0.26 +1.6
311,265 - CCNA_00297 0.26 +2.5
311,265 - CCNA_00297 0.26 +0.8
311,267 + CCNA_00297 0.27 +0.5
311,267 + CCNA_00297 0.27 -0.7
311,267 + CCNA_00297 0.27 -1.5
311,275 - CCNA_00297 0.28 +0.1
311,275 - CCNA_00297 0.28 +1.9
311,275 - CCNA_00297 0.28 +0.8
311,275 - CCNA_00297 0.28 -0.8
311,275 - CCNA_00297 0.28 +0.3
311,282 + CCNA_00297 0.28 -0.7
311,282 + CCNA_00297 0.28 -0.1
311,290 - CCNA_00297 0.29 +0.0
311,290 - CCNA_00297 0.29 -1.2
311,329 - CCNA_00297 0.34 -0.0
311,329 - CCNA_00297 0.34 +0.8
311,349 + CCNA_00297 0.36 -1.4
311,360 - CCNA_00297 0.37 +1.3
311,366 + CCNA_00297 0.38 -1.1
311,381 + CCNA_00297 0.40 +0.8
311,408 + CCNA_00297 0.43 -1.7
311,408 + CCNA_00297 0.43 -0.3
311,416 - CCNA_00297 0.44 -1.5
311,416 - CCNA_00297 0.44 -1.2
311,416 - CCNA_00297 0.44 -0.7
311,416 - CCNA_00297 0.44 +0.0
311,445 + CCNA_00297 0.47 -0.5
311,453 - CCNA_00297 0.48 -0.3
311,494 - CCNA_00297 0.53 -0.6
311,577 - CCNA_00297 0.63 +1.5
311,577 - CCNA_00297 0.63 -0.8
311,594 + CCNA_00297 0.65 +1.3
311,602 - CCNA_00297 0.66 -1.0
311,602 - CCNA_00297 0.66 -2.3
311,602 - CCNA_00297 0.66 -1.5
311,632 - CCNA_00297 0.69 -2.2
311,670 + CCNA_00297 0.74 +1.3
311,690 - CCNA_00297 0.76 +0.4
311,690 - CCNA_00297 0.76 -2.6
311,690 - CCNA_00297 0.76 +0.8
311,706 - CCNA_00297 0.78 +0.2
311,741 + CCNA_00297 0.82 -1.5
311,741 + CCNA_00297 0.82 +0.3
311,751 + CCNA_00297 0.83 -2.5
311,754 - CCNA_00297 0.83 +1.3
311,765 - CCNA_00297 0.85 -1.7
311,772 + CCNA_00297 0.85 -1.8
311,775 - CCNA_00297 0.86 -0.9
311,846 + -1.4
311,846 + +0.8
311,846 + -0.8
311,846 + +0.8
311,854 - -0.2
311,889 + -0.5
311,897 - -3.5
311,897 - +0.3
311,900 + +0.0
311,936 + -0.7
311,954 + +0.3
311,954 + +0.3
311,962 + +1.4
311,962 + +1.5
311,962 - -0.5
311,967 + -1.1
311,980 - CCNA_00298 0.11 -1.1
312,004 + CCNA_00298 0.14 -0.2
312,033 + CCNA_00298 0.18 +2.3
312,041 - CCNA_00298 0.19 -0.2

Or see this region's nucleotide sequence