Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00268 and CCNA_00269 are separated by 145 nucleotides CCNA_00269 and CCNA_00270 are separated by 126 nucleotides CCNA_00270 and CCNA_00271 are separated by 109 nucleotides CCNA_00271 and CCNA_00272 are separated by 53 nucleotides
CCNA_00268: CCNA_00268 - DNA polymerase III subunit gamma/tau, at 282,130 to 283,956
_00268
CCNA_00269: CCNA_00269 - YbaB/EbfC-family DNA-binding protein, at 284,102 to 284,431
_00269
CCNA_00270: CCNA_00270 - Recombination protein recR, at 284,558 to 285,160
_00270
CCNA_00271: CCNA_00271 - hypothetical protein, at 285,270 to 285,422
_00271
CCNA_00272: CCNA_00272 - RmuC family protein, at 285,476 to 286,765
_00272
Position (kb) 284
285
286 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 283.924 kb on + strand at 283.979 kb on - strand at 283.988 kb on + strand at 283.988 kb on + strand at 283.989 kb on - strand at 283.996 kb on - strand at 283.996 kb on - strand at 283.996 kb on - strand at 283.996 kb on - strand at 283.996 kb on - strand at 283.996 kb on - strand at 283.996 kb on - strand at 283.996 kb on - strand at 284.032 kb on + strand at 284.050 kb on + strand at 284.058 kb on - strand at 284.058 kb on - strand at 284.058 kb on - strand at 284.058 kb on - strand at 284.058 kb on - strand at 284.058 kb on - strand at 284.059 kb on - strand at 284.108 kb on - strand at 284.121 kb on + strand at 284.122 kb on + strand at 284.131 kb on + strand at 284.131 kb on + strand at 284.131 kb on + strand at 284.131 kb on + strand at 284.131 kb on + strand at 284.131 kb on + strand at 284.139 kb on - strand, within CCNA_00269 at 284.139 kb on - strand, within CCNA_00269 at 284.139 kb on - strand at 284.139 kb on - strand, within CCNA_00269 at 284.139 kb on - strand, within CCNA_00269 at 284.139 kb on - strand, within CCNA_00269 at 284.140 kb on - strand, within CCNA_00269 at 284.182 kb on + strand, within CCNA_00269 at 284.182 kb on + strand, within CCNA_00269 at 284.185 kb on - strand, within CCNA_00269 at 284.220 kb on + strand, within CCNA_00269 at 284.220 kb on + strand, within CCNA_00269 at 284.228 kb on + strand, within CCNA_00269 at 284.228 kb on - strand, within CCNA_00269 at 284.238 kb on + strand, within CCNA_00269 at 284.239 kb on + strand, within CCNA_00269 at 284.240 kb on + strand, within CCNA_00269 at 284.260 kb on + strand, within CCNA_00269 at 284.290 kb on + strand, within CCNA_00269 at 284.291 kb on + strand, within CCNA_00269 at 284.309 kb on - strand, within CCNA_00269 at 284.311 kb on + strand, within CCNA_00269 at 284.314 kb on - strand, within CCNA_00269 at 284.319 kb on - strand, within CCNA_00269 at 284.338 kb on + strand, within CCNA_00269 at 284.349 kb on + strand, within CCNA_00269 at 284.359 kb on + strand, within CCNA_00269 at 284.359 kb on + strand, within CCNA_00269 at 284.377 kb on - strand, within CCNA_00269 at 284.425 kb on + strand at 284.448 kb on + strand at 284.462 kb on - strand at 284.462 kb on - strand at 284.464 kb on + strand at 284.464 kb on + strand at 284.464 kb on + strand at 284.465 kb on + strand at 284.470 kb on + strand at 284.470 kb on + strand at 284.470 kb on + strand at 284.470 kb on + strand at 284.470 kb on + strand at 284.470 kb on + strand at 284.472 kb on - strand at 284.478 kb on - strand at 284.478 kb on - strand at 284.478 kb on - strand at 284.478 kb on - strand at 284.478 kb on - strand at 284.482 kb on + strand at 284.482 kb on + strand at 284.485 kb on - strand at 284.490 kb on - strand at 284.505 kb on + strand at 284.505 kb on + strand at 284.505 kb on + strand at 284.513 kb on - strand at 284.513 kb on - strand at 284.514 kb on - strand at 284.524 kb on - strand at 284.538 kb on + strand at 284.554 kb on + strand at 284.569 kb on + strand at 284.569 kb on + strand at 284.613 kb on - strand at 284.648 kb on + strand, within CCNA_00270 at 284.648 kb on + strand, within CCNA_00270 at 284.656 kb on - strand, within CCNA_00270 at 284.656 kb on - strand, within CCNA_00270 at 284.656 kb on - strand, within CCNA_00270 at 284.656 kb on - strand, within CCNA_00270 at 284.695 kb on - strand, within CCNA_00270 at 284.716 kb on + strand, within CCNA_00270 at 284.716 kb on + strand, within CCNA_00270 at 284.724 kb on - strand, within CCNA_00270 at 284.724 kb on - strand, within CCNA_00270 at 284.806 kb on + strand, within CCNA_00270 at 284.841 kb on - strand, within CCNA_00270 at 284.841 kb on - strand, within CCNA_00270 at 284.841 kb on - strand, within CCNA_00270 at 284.842 kb on - strand, within CCNA_00270 at 284.842 kb on - strand, within CCNA_00270 at 284.846 kb on - strand, within CCNA_00270 at 284.846 kb on - strand, within CCNA_00270 at 284.884 kb on + strand, within CCNA_00270 at 284.905 kb on + strand, within CCNA_00270 at 284.910 kb on + strand, within CCNA_00270 at 285.002 kb on + strand, within CCNA_00270 at 285.020 kb on + strand, within CCNA_00270 at 285.044 kb on + strand, within CCNA_00270 at 285.044 kb on + strand, within CCNA_00270 at 285.051 kb on - strand, within CCNA_00270 at 285.089 kb on - strand, within CCNA_00270 at 285.241 kb on + strand at 285.248 kb on + strand at 285.249 kb on - strand at 285.282 kb on + strand at 285.285 kb on - strand at 285.326 kb on - strand, within CCNA_00271 at 285.349 kb on - strand, within CCNA_00271 at 285.354 kb on + strand, within CCNA_00271 at 285.405 kb on + strand, within CCNA_00271 at 285.447 kb on - strand at 285.464 kb on - strand at 285.542 kb on - strand at 285.565 kb on - strand at 285.577 kb on + strand at 285.578 kb on + strand at 285.584 kb on + strand at 285.585 kb on - strand at 285.589 kb on + strand at 285.590 kb on + strand at 285.592 kb on - strand at 285.609 kb on + strand, within CCNA_00272 at 285.613 kb on + strand, within CCNA_00272 at 285.616 kb on - strand, within CCNA_00272 at 285.655 kb on - strand, within CCNA_00272 at 285.673 kb on + strand, within CCNA_00272 at 285.673 kb on + strand, within CCNA_00272 at 285.673 kb on + strand, within CCNA_00272 at 285.673 kb on + strand, within CCNA_00272 at 285.673 kb on + strand, within CCNA_00272 at 285.673 kb on + strand, within CCNA_00272 at 285.673 kb on + strand, within CCNA_00272 at 285.676 kb on - strand, within CCNA_00272 at 285.679 kb on + strand, within CCNA_00272 at 285.681 kb on - strand, within CCNA_00272 at 285.681 kb on - strand, within CCNA_00272 at 285.681 kb on - strand, within CCNA_00272 at 285.681 kb on - strand, within CCNA_00272 at 285.711 kb on - strand, within CCNA_00272 at 285.729 kb on - strand, within CCNA_00272 at 285.783 kb on - strand, within CCNA_00272 at 285.783 kb on - strand, within CCNA_00272 at 285.787 kb on + strand, within CCNA_00272 at 285.787 kb on + strand, within CCNA_00272 at 285.795 kb on - strand, within CCNA_00272 at 285.795 kb on - strand, within CCNA_00272 at 285.795 kb on - strand, within CCNA_00272 at 285.795 kb on - strand, within CCNA_00272 at 285.811 kb on + strand, within CCNA_00272 at 285.829 kb on + strand, within CCNA_00272 at 285.837 kb on - strand, within CCNA_00272 at 285.847 kb on + strand, within CCNA_00272 at 285.855 kb on - strand, within CCNA_00272 at 285.883 kb on + strand, within CCNA_00272 at 285.893 kb on + strand, within CCNA_00272 at 285.935 kb on - strand, within CCNA_00272 at 286.029 kb on - strand, within CCNA_00272 at 286.048 kb on + strand, within CCNA_00272 at 286.048 kb on + strand, within CCNA_00272 at 286.048 kb on + strand, within CCNA_00272 at 286.100 kb on - strand, within CCNA_00272
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml remove 283,924 + +2.2 283,979 - +0.6 283,988 + +3.7 283,988 + +1.0 283,989 - -0.3 283,996 - -0.3 283,996 - -1.1 283,996 - +0.0 283,996 - -1.0 283,996 - +0.0 283,996 - -0.2 283,996 - -0.4 283,996 - +1.5 284,032 + +1.5 284,050 + +1.8 284,058 - -3.0 284,058 - -1.1 284,058 - +1.2 284,058 - +0.8 284,058 - -0.2 284,058 - -0.2 284,059 - +1.1 284,108 - -2.9 284,121 + +0.3 284,122 + -1.0 284,131 + -0.6 284,131 + +2.7 284,131 + -1.1 284,131 + -1.0 284,131 + +1.4 284,131 + +0.9 284,139 - CCNA_00269 0.11 +2.6 284,139 - CCNA_00269 0.11 +2.6 284,139 - +3.4 284,139 - CCNA_00269 0.11 +3.1 284,139 - CCNA_00269 0.11 +2.4 284,139 - CCNA_00269 0.11 +2.5 284,140 - CCNA_00269 0.12 +3.2 284,182 + CCNA_00269 0.24 +1.5 284,182 + CCNA_00269 0.24 +2.2 284,185 - CCNA_00269 0.25 -1.4 284,220 + CCNA_00269 0.36 +0.4 284,220 + CCNA_00269 0.36 +0.5 284,228 + CCNA_00269 0.38 -0.4 284,228 - CCNA_00269 0.38 +2.4 284,238 + CCNA_00269 0.41 +2.6 284,239 + CCNA_00269 0.42 +0.6 284,240 + CCNA_00269 0.42 +0.4 284,260 + CCNA_00269 0.48 +1.0 284,290 + CCNA_00269 0.57 +0.6 284,291 + CCNA_00269 0.57 +0.2 284,309 - CCNA_00269 0.63 -0.6 284,311 + CCNA_00269 0.63 +1.0 284,314 - CCNA_00269 0.64 +1.4 284,319 - CCNA_00269 0.66 -0.2 284,338 + CCNA_00269 0.72 +0.2 284,349 + CCNA_00269 0.75 -0.1 284,359 + CCNA_00269 0.78 +1.7 284,359 + CCNA_00269 0.78 +2.0 284,377 - CCNA_00269 0.83 +2.4 284,425 + +1.9 284,448 + +1.8 284,462 - +1.0 284,462 - -0.6 284,464 + +2.3 284,464 + +2.0 284,464 + +1.6 284,465 + +1.6 284,470 + +0.4 284,470 + +0.2 284,470 + +2.0 284,470 + +1.3 284,470 + +1.5 284,470 + +1.1 284,472 - +2.4 284,478 - +3.1 284,478 - +1.5 284,478 - +2.4 284,478 - +1.8 284,478 - +2.3 284,482 + +1.2 284,482 + +1.3 284,485 - +1.4 284,490 - -0.2 284,505 + +0.2 284,505 + +0.9 284,505 + +1.0 284,513 - +1.8 284,513 - -0.9 284,514 - +0.2 284,524 - +2.1 284,538 + +1.2 284,554 + +0.6 284,569 + +1.6 284,569 + +1.7 284,613 - +1.4 284,648 + CCNA_00270 0.15 +1.4 284,648 + CCNA_00270 0.15 +1.1 284,656 - CCNA_00270 0.16 +1.2 284,656 - CCNA_00270 0.16 -0.7 284,656 - CCNA_00270 0.16 -1.1 284,656 - CCNA_00270 0.16 -0.3 284,695 - CCNA_00270 0.23 -0.4 284,716 + CCNA_00270 0.26 +0.5 284,716 + CCNA_00270 0.26 +0.9 284,724 - CCNA_00270 0.28 +0.3 284,724 - CCNA_00270 0.28 +0.4 284,806 + CCNA_00270 0.41 +2.7 284,841 - CCNA_00270 0.47 +1.4 284,841 - CCNA_00270 0.47 +0.8 284,841 - CCNA_00270 0.47 -1.5 284,842 - CCNA_00270 0.47 +0.0 284,842 - CCNA_00270 0.47 -0.1 284,846 - CCNA_00270 0.48 +0.0 284,846 - CCNA_00270 0.48 -1.0 284,884 + CCNA_00270 0.54 +0.1 284,905 + CCNA_00270 0.58 +1.9 284,910 + CCNA_00270 0.58 +2.9 285,002 + CCNA_00270 0.74 +0.5 285,020 + CCNA_00270 0.77 +1.3 285,044 + CCNA_00270 0.81 +0.8 285,044 + CCNA_00270 0.81 +0.8 285,051 - CCNA_00270 0.82 -2.2 285,089 - CCNA_00270 0.88 +0.8 285,241 + -0.4 285,248 + +2.4 285,249 - -1.9 285,282 + -0.1 285,285 - -1.4 285,326 - CCNA_00271 0.37 +1.8 285,349 - CCNA_00271 0.52 +0.6 285,354 + CCNA_00271 0.55 +0.0 285,405 + CCNA_00271 0.88 +0.9 285,447 - -0.6 285,464 - -1.8 285,542 - -0.3 285,565 - -0.2 285,577 + -0.8 285,578 + -1.1 285,584 + -2.5 285,585 - -1.3 285,589 + -0.9 285,590 + -0.7 285,592 - -0.0 285,609 + CCNA_00272 0.10 -1.0 285,613 + CCNA_00272 0.11 +0.8 285,616 - CCNA_00272 0.11 -1.5 285,655 - CCNA_00272 0.14 +2.4 285,673 + CCNA_00272 0.15 +1.2 285,673 + CCNA_00272 0.15 -0.2 285,673 + CCNA_00272 0.15 -0.9 285,673 + CCNA_00272 0.15 +0.9 285,673 + CCNA_00272 0.15 -0.3 285,673 + CCNA_00272 0.15 -0.4 285,673 + CCNA_00272 0.15 -0.2 285,676 - CCNA_00272 0.16 -2.1 285,679 + CCNA_00272 0.16 +1.4 285,681 - CCNA_00272 0.16 +0.3 285,681 - CCNA_00272 0.16 +1.2 285,681 - CCNA_00272 0.16 -0.6 285,681 - CCNA_00272 0.16 -1.5 285,711 - CCNA_00272 0.18 +1.1 285,729 - CCNA_00272 0.20 +0.5 285,783 - CCNA_00272 0.24 -3.4 285,783 - CCNA_00272 0.24 +0.5 285,787 + CCNA_00272 0.24 -1.4 285,787 + CCNA_00272 0.24 -1.0 285,795 - CCNA_00272 0.25 +0.8 285,795 - CCNA_00272 0.25 -0.4 285,795 - CCNA_00272 0.25 -0.6 285,795 - CCNA_00272 0.25 -2.5 285,811 + CCNA_00272 0.26 -1.6 285,829 + CCNA_00272 0.27 +0.0 285,837 - CCNA_00272 0.28 -1.2 285,847 + CCNA_00272 0.29 +0.9 285,855 - CCNA_00272 0.29 +1.2 285,883 + CCNA_00272 0.32 -0.6 285,893 + CCNA_00272 0.32 +3.4 285,935 - CCNA_00272 0.36 -1.4 286,029 - CCNA_00272 0.43 -1.2 286,048 + CCNA_00272 0.44 +0.1 286,048 + CCNA_00272 0.44 -1.3 286,048 + CCNA_00272 0.44 -0.7 286,100 - CCNA_00272 0.48 -2.4
Or see this region's nucleotide sequence