Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00154

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00152 and CCNA_00153 are separated by 12 nucleotidesCCNA_00153 and CCNA_00154 overlap by 61 nucleotidesCCNA_00154 and CCNA_00155 are separated by 177 nucleotides CCNA_00152: CCNA_00152 - winged helix-turn-helix transcription repressor HrcA, at 161,320 to 162,396 _00152 CCNA_00153: CCNA_00153 - adenine nucleotide exchange factor GrpE, at 162,409 to 163,035 _00153 CCNA_00154: CCNA_00154 - hypothetical protein, at 162,975 to 163,235 _00154 CCNA_00155: CCNA_00155 - DNA polymerase III, beta chain, at 163,413 to 164,531 _00155 Position (kb) 162 163 164Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 162.025 kb on + strand, within CCNA_00152at 162.064 kb on - strand, within CCNA_00152at 162.075 kb on + strand, within CCNA_00152at 162.122 kb on + strand, within CCNA_00152at 162.140 kb on + strand, within CCNA_00152at 162.140 kb on + strand, within CCNA_00152at 162.147 kb on + strand, within CCNA_00152at 162.171 kb on + strand, within CCNA_00152at 162.171 kb on + strand, within CCNA_00152at 162.171 kb on + strand, within CCNA_00152at 162.171 kb on + strand, within CCNA_00152at 162.179 kb on - strand, within CCNA_00152at 162.239 kb on - strand, within CCNA_00152at 162.264 kb on + strand, within CCNA_00152at 162.267 kb on + strand, within CCNA_00152at 162.269 kb on + strand, within CCNA_00152at 162.340 kb on - strandat 162.347 kb on - strandat 162.354 kb on + strandat 162.392 kb on + strandat 162.392 kb on + strandat 162.392 kb on + strandat 162.405 kb on - strandat 162.937 kb on - strand, within CCNA_00153at 162.952 kb on - strand, within CCNA_00153at 162.972 kb on + strand, within CCNA_00153at 162.973 kb on - strandat 162.980 kb on - strandat 162.981 kb on - strandat 163.031 kb on - strandat 163.104 kb on + strand, within CCNA_00154at 163.112 kb on - strand, within CCNA_00154at 163.113 kb on - strand, within CCNA_00154at 163.117 kb on + strand, within CCNA_00154at 163.117 kb on + strandat 163.117 kb on + strand, within CCNA_00154at 163.158 kb on + strand, within CCNA_00154at 163.165 kb on + strand, within CCNA_00154at 163.171 kb on - strand, within CCNA_00154at 163.171 kb on - strand, within CCNA_00154at 163.178 kb on - strand, within CCNA_00154at 163.209 kb on + strand, within CCNA_00154at 163.209 kb on + strand, within CCNA_00154at 163.209 kb on + strand, within CCNA_00154at 163.209 kb on + strand, within CCNA_00154at 163.209 kb on + strand, within CCNA_00154at 163.209 kb on + strand, within CCNA_00154at 163.217 kb on - strandat 163.217 kb on - strandat 163.217 kb on - strandat 163.217 kb on - strandat 163.217 kb on - strandat 163.217 kb on - strandat 163.217 kb on - strandat 163.218 kb on + strandat 163.218 kb on + strandat 163.218 kb on + strandat 163.223 kb on + strandat 163.226 kb on - strandat 163.226 kb on - strandat 163.226 kb on - strandat 163.231 kb on - strandat 163.233 kb on + strandat 163.289 kb on - strandat 163.297 kb on - strandat 163.304 kb on - strandat 163.348 kb on + strandat 163.365 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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162,025 + CCNA_00152 0.65 +2.0
162,064 - CCNA_00152 0.69 -1.3
162,075 + CCNA_00152 0.70 +2.0
162,122 + CCNA_00152 0.74 +1.9
162,140 + CCNA_00152 0.76 +0.8
162,140 + CCNA_00152 0.76 +1.4
162,147 + CCNA_00152 0.77 +0.4
162,171 + CCNA_00152 0.79 +0.5
162,171 + CCNA_00152 0.79 -1.2
162,171 + CCNA_00152 0.79 +1.8
162,171 + CCNA_00152 0.79 +2.4
162,179 - CCNA_00152 0.80 +0.0
162,239 - CCNA_00152 0.85 -0.5
162,264 + CCNA_00152 0.88 +1.4
162,267 + CCNA_00152 0.88 +2.8
162,269 + CCNA_00152 0.88 +1.4
162,340 - -1.3
162,347 - -1.3
162,354 + +1.5
162,392 + +1.1
162,392 + +1.7
162,392 + +0.9
162,405 - -0.6
162,937 - CCNA_00153 0.84 -0.6
162,952 - CCNA_00153 0.87 +1.1
162,972 + CCNA_00153 0.90 +2.5
162,973 - +1.2
162,980 - +0.4
162,981 - +0.8
163,031 - +0.8
163,104 + CCNA_00154 0.49 +2.0
163,112 - CCNA_00154 0.52 +0.5
163,113 - CCNA_00154 0.53 -0.0
163,117 + CCNA_00154 0.54 +0.7
163,117 + +4.9
163,117 + CCNA_00154 0.54 +1.2
163,158 + CCNA_00154 0.70 -1.2
163,165 + CCNA_00154 0.73 -0.2
163,171 - CCNA_00154 0.75 +0.7
163,171 - CCNA_00154 0.75 -1.7
163,178 - CCNA_00154 0.78 +1.1
163,209 + CCNA_00154 0.90 +0.2
163,209 + CCNA_00154 0.90 +0.6
163,209 + CCNA_00154 0.90 +1.4
163,209 + CCNA_00154 0.90 +2.4
163,209 + CCNA_00154 0.90 +0.7
163,209 + CCNA_00154 0.90 +0.3
163,217 - +0.3
163,217 - +1.1
163,217 - -0.7
163,217 - -0.3
163,217 - +0.7
163,217 - +0.0
163,217 - -0.5
163,218 + -1.2
163,218 + +2.4
163,218 + +1.6
163,223 + -2.5
163,226 - -1.1
163,226 - -1.3
163,226 - -0.3
163,231 - +0.5
163,233 + -0.9
163,289 - +2.0
163,297 - +0.0
163,304 - +3.4
163,348 + -0.8
163,365 + +0.8

Or see this region's nucleotide sequence