Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00031

Experiment: PYE with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00029 and CCNA_00030 are separated by 48 nucleotidesCCNA_00030 and CCNA_00031 overlap by 18 nucleotidesCCNA_00031 and CCNA_00032 are separated by 0 nucleotidesCCNA_00032 and CCNA_03912 are separated by 105 nucleotides CCNA_00029: CCNA_00029 - lysine exporter protein, at 32,914 to 33,546 _00029 CCNA_00030: CCNA_00030 - ribonuclease T2, at 33,595 to 34,329 _00030 CCNA_00031: CCNA_00031 - toxin protein VapC, at 34,312 to 34,698 _00031 CCNA_00032: CCNA_00032 - antitoxin protein VapB, at 34,699 to 34,941 _00032 CCNA_03912: CCNA_03912 - hypothetical protein, at 35,047 to 35,667 _03912 Position (kb) 34 35Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 33.335 kb on - strandat 33.358 kb on - strand, within CCNA_00029at 33.367 kb on + strand, within CCNA_00029at 33.370 kb on - strand, within CCNA_00029at 33.379 kb on + strand, within CCNA_00029at 33.379 kb on + strand, within CCNA_00029at 33.382 kb on - strand, within CCNA_00029at 33.384 kb on + strand, within CCNA_00029at 33.403 kb on - strand, within CCNA_00029at 33.456 kb on + strand, within CCNA_00029at 33.472 kb on - strand, within CCNA_00029at 33.499 kb on + strandat 33.499 kb on + strandat 33.499 kb on - strandat 33.499 kb on - strandat 33.562 kb on - strandat 33.565 kb on + strandat 33.671 kb on + strand, within CCNA_00030at 33.677 kb on - strand, within CCNA_00030at 33.679 kb on - strand, within CCNA_00030at 33.684 kb on + strand, within CCNA_00030at 33.684 kb on + strand, within CCNA_00030at 33.687 kb on - strand, within CCNA_00030at 33.687 kb on - strand, within CCNA_00030at 33.692 kb on - strand, within CCNA_00030at 33.692 kb on - strand, within CCNA_00030at 33.704 kb on - strand, within CCNA_00030at 33.749 kb on + strand, within CCNA_00030at 33.757 kb on - strand, within CCNA_00030at 33.757 kb on - strand, within CCNA_00030at 33.777 kb on - strand, within CCNA_00030at 33.781 kb on + strand, within CCNA_00030at 33.789 kb on - strand, within CCNA_00030at 33.789 kb on - strand, within CCNA_00030at 33.794 kb on - strand, within CCNA_00030at 33.796 kb on + strand, within CCNA_00030at 33.796 kb on + strand, within CCNA_00030at 33.804 kb on - strand, within CCNA_00030at 33.825 kb on - strand, within CCNA_00030at 33.856 kb on + strand, within CCNA_00030at 33.871 kb on + strand, within CCNA_00030at 33.871 kb on + strand, within CCNA_00030at 33.871 kb on - strand, within CCNA_00030at 33.879 kb on - strand, within CCNA_00030at 33.879 kb on - strand, within CCNA_00030at 33.879 kb on - strand, within CCNA_00030at 33.917 kb on + strand, within CCNA_00030at 33.942 kb on + strand, within CCNA_00030at 33.950 kb on - strand, within CCNA_00030at 33.974 kb on - strand, within CCNA_00030at 33.974 kb on - strand, within CCNA_00030at 33.999 kb on + strand, within CCNA_00030at 34.007 kb on - strand, within CCNA_00030at 34.012 kb on + strand, within CCNA_00030at 34.042 kb on - strand, within CCNA_00030at 34.068 kb on - strand, within CCNA_00030at 34.135 kb on + strand, within CCNA_00030at 34.135 kb on + strand, within CCNA_00030at 34.155 kb on + strand, within CCNA_00030at 34.172 kb on + strand, within CCNA_00030at 34.176 kb on + strand, within CCNA_00030at 34.177 kb on + strand, within CCNA_00030at 34.180 kb on - strand, within CCNA_00030at 34.180 kb on - strand, within CCNA_00030at 34.184 kb on - strand, within CCNA_00030at 34.191 kb on + strand, within CCNA_00030at 34.199 kb on - strand, within CCNA_00030at 34.203 kb on - strand, within CCNA_00030at 34.214 kb on + strand, within CCNA_00030at 34.224 kb on - strand, within CCNA_00030at 34.226 kb on + strand, within CCNA_00030at 34.234 kb on - strand, within CCNA_00030at 34.239 kb on - strand, within CCNA_00030at 34.278 kb on - strandat 34.316 kb on - strandat 34.387 kb on + strand, within CCNA_00031at 34.388 kb on + strand, within CCNA_00031at 34.403 kb on - strand, within CCNA_00031at 34.414 kb on + strand, within CCNA_00031at 34.434 kb on - strand, within CCNA_00031at 34.455 kb on - strand, within CCNA_00031at 34.526 kb on - strand, within CCNA_00031at 34.526 kb on - strandat 34.544 kb on + strand, within CCNA_00031at 34.552 kb on - strand, within CCNA_00031at 34.568 kb on + strand, within CCNA_00031at 34.581 kb on - strand, within CCNA_00031at 34.600 kb on - strand, within CCNA_00031at 34.615 kb on + strand, within CCNA_00031at 34.615 kb on + strand, within CCNA_00031at 34.615 kb on + strand, within CCNA_00031at 34.615 kb on + strand, within CCNA_00031at 34.622 kb on + strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.623 kb on - strand, within CCNA_00031at 34.630 kb on - strand, within CCNA_00031at 34.630 kb on - strand, within CCNA_00031at 34.630 kb on - strand, within CCNA_00031at 34.705 kb on - strandat 34.819 kb on - strand, within CCNA_00032at 34.824 kb on - strand, within CCNA_00032at 34.824 kb on - strand, within CCNA_00032at 34.824 kb on - strand, within CCNA_00032at 34.824 kb on - strand, within CCNA_00032at 34.856 kb on - strand, within CCNA_00032at 34.867 kb on + strand, within CCNA_00032at 34.992 kb on - strandat 35.019 kb on + strandat 35.026 kb on + strandat 35.055 kb on + strandat 35.055 kb on + strandat 35.151 kb on + strand, within CCNA_03912at 35.174 kb on - strand, within CCNA_03912at 35.189 kb on - strand, within CCNA_03912at 35.265 kb on - strand, within CCNA_03912at 35.279 kb on + strand, within CCNA_03912at 35.282 kb on - strand, within CCNA_03912at 35.300 kb on + strand, within CCNA_03912at 35.353 kb on + strand, within CCNA_03912at 35.361 kb on - strand, within CCNA_03912at 35.361 kb on - strand, within CCNA_03912at 35.397 kb on - strand, within CCNA_03912at 35.425 kb on + strand, within CCNA_03912at 35.425 kb on + strand, within CCNA_03912at 35.487 kb on + strand, within CCNA_03912at 35.491 kb on - strand, within CCNA_03912at 35.501 kb on + strand, within CCNA_03912at 35.514 kb on - strand, within CCNA_03912at 35.535 kb on + strand, within CCNA_03912at 35.558 kb on + strand, within CCNA_03912at 35.558 kb on + strand, within CCNA_03912at 35.585 kb on + strand, within CCNA_03912at 35.592 kb on + strand, within CCNA_03912at 35.593 kb on + strand, within CCNA_03912at 35.604 kb on + strand, within CCNA_03912at 35.612 kb on - strandat 35.612 kb on - strandat 35.623 kb on - strandat 35.639 kb on + strandat 35.658 kb on - strandat 35.661 kb on + strandat 35.669 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Polymyxin B sulfate 0.005 mg/ml
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33,335 - +1.4
33,358 - CCNA_00029 0.70 -0.2
33,367 + CCNA_00029 0.72 +0.4
33,370 - CCNA_00029 0.72 +0.8
33,379 + CCNA_00029 0.73 -1.3
33,379 + CCNA_00029 0.73 +1.1
33,382 - CCNA_00029 0.74 -1.1
33,384 + CCNA_00029 0.74 +0.8
33,403 - CCNA_00029 0.77 -1.3
33,456 + CCNA_00029 0.86 +0.0
33,472 - CCNA_00029 0.88 +0.8
33,499 + -1.5
33,499 + +0.0
33,499 - +0.8
33,499 - +2.4
33,562 - -1.7
33,565 + +2.4
33,671 + CCNA_00030 0.10 -1.1
33,677 - CCNA_00030 0.11 -0.9
33,679 - CCNA_00030 0.11 +0.8
33,684 + CCNA_00030 0.12 -1.0
33,684 + CCNA_00030 0.12 -2.0
33,687 - CCNA_00030 0.13 -1.7
33,687 - CCNA_00030 0.13 -1.2
33,692 - CCNA_00030 0.13 -1.2
33,692 - CCNA_00030 0.13 -0.4
33,704 - CCNA_00030 0.15 +0.0
33,749 + CCNA_00030 0.21 +1.4
33,757 - CCNA_00030 0.22 -0.2
33,757 - CCNA_00030 0.22 -0.6
33,777 - CCNA_00030 0.25 -0.5
33,781 + CCNA_00030 0.25 -0.2
33,789 - CCNA_00030 0.26 -1.5
33,789 - CCNA_00030 0.26 -1.5
33,794 - CCNA_00030 0.27 +0.0
33,796 + CCNA_00030 0.27 +1.8
33,796 + CCNA_00030 0.27 -1.3
33,804 - CCNA_00030 0.28 +2.4
33,825 - CCNA_00030 0.31 +0.8
33,856 + CCNA_00030 0.36 -1.3
33,871 + CCNA_00030 0.38 -0.7
33,871 + CCNA_00030 0.38 -0.1
33,871 - CCNA_00030 0.38 +0.4
33,879 - CCNA_00030 0.39 +2.4
33,879 - CCNA_00030 0.39 -2.0
33,879 - CCNA_00030 0.39 -0.5
33,917 + CCNA_00030 0.44 +0.4
33,942 + CCNA_00030 0.47 -0.2
33,950 - CCNA_00030 0.48 +0.5
33,974 - CCNA_00030 0.52 +1.2
33,974 - CCNA_00030 0.52 -0.2
33,999 + CCNA_00030 0.55 -0.8
34,007 - CCNA_00030 0.56 -0.8
34,012 + CCNA_00030 0.57 -1.2
34,042 - CCNA_00030 0.61 +1.8
34,068 - CCNA_00030 0.64 -1.1
34,135 + CCNA_00030 0.73 +1.1
34,135 + CCNA_00030 0.73 +0.0
34,155 + CCNA_00030 0.76 +1.0
34,172 + CCNA_00030 0.79 -1.0
34,176 + CCNA_00030 0.79 +1.9
34,177 + CCNA_00030 0.79 -1.9
34,180 - CCNA_00030 0.80 -1.0
34,180 - CCNA_00030 0.80 +0.6
34,184 - CCNA_00030 0.80 -1.2
34,191 + CCNA_00030 0.81 +0.0
34,199 - CCNA_00030 0.82 +1.2
34,203 - CCNA_00030 0.83 -0.6
34,214 + CCNA_00030 0.84 +3.9
34,224 - CCNA_00030 0.86 -1.2
34,226 + CCNA_00030 0.86 +0.8
34,234 - CCNA_00030 0.87 +0.7
34,239 - CCNA_00030 0.88 +1.9
34,278 - -0.1
34,316 - -0.0
34,387 + CCNA_00031 0.19 +0.8
34,388 + CCNA_00031 0.20 -0.8
34,403 - CCNA_00031 0.24 +1.8
34,414 + CCNA_00031 0.26 +1.4
34,434 - CCNA_00031 0.32 +3.0
34,455 - CCNA_00031 0.37 +2.0
34,526 - CCNA_00031 0.55 +3.9
34,526 - +3.9
34,544 + CCNA_00031 0.60 -2.1
34,552 - CCNA_00031 0.62 +2.9
34,568 + CCNA_00031 0.66 -0.1
34,581 - CCNA_00031 0.70 +2.8
34,600 - CCNA_00031 0.74 +2.8
34,615 + CCNA_00031 0.78 -0.8
34,615 + CCNA_00031 0.78 +0.4
34,615 + CCNA_00031 0.78 +0.0
34,615 + CCNA_00031 0.78 -1.2
34,622 + CCNA_00031 0.80 -2.0
34,623 - CCNA_00031 0.80 +2.6
34,623 - CCNA_00031 0.80 +3.0
34,623 - CCNA_00031 0.80 +2.9
34,623 - CCNA_00031 0.80 +2.6
34,623 - CCNA_00031 0.80 +2.6
34,623 - CCNA_00031 0.80 +3.1
34,623 - CCNA_00031 0.80 +3.0
34,623 - CCNA_00031 0.80 +3.4
34,623 - CCNA_00031 0.80 +2.8
34,623 - CCNA_00031 0.80 +3.0
34,623 - CCNA_00031 0.80 +3.2
34,630 - CCNA_00031 0.82 +3.6
34,630 - CCNA_00031 0.82 +3.5
34,630 - CCNA_00031 0.82 +2.7
34,705 - -1.8
34,819 - CCNA_00032 0.49 +3.1
34,824 - CCNA_00032 0.51 +1.9
34,824 - CCNA_00032 0.51 +2.4
34,824 - CCNA_00032 0.51 +0.3
34,824 - CCNA_00032 0.51 +2.5
34,856 - CCNA_00032 0.65 +2.2
34,867 + CCNA_00032 0.69 -0.6
34,992 - -0.5
35,019 + -0.5
35,026 + -2.5
35,055 + -1.3
35,055 + -0.6
35,151 + CCNA_03912 0.17 -1.3
35,174 - CCNA_03912 0.20 +0.2
35,189 - CCNA_03912 0.23 -1.4
35,265 - CCNA_03912 0.35 -0.2
35,279 + CCNA_03912 0.37 -1.2
35,282 - CCNA_03912 0.38 -1.0
35,300 + CCNA_03912 0.41 -0.5
35,353 + CCNA_03912 0.49 -1.0
35,361 - CCNA_03912 0.51 -1.0
35,361 - CCNA_03912 0.51 +0.7
35,397 - CCNA_03912 0.56 -1.5
35,425 + CCNA_03912 0.61 +1.1
35,425 + CCNA_03912 0.61 -1.2
35,487 + CCNA_03912 0.71 -0.1
35,491 - CCNA_03912 0.71 -0.6
35,501 + CCNA_03912 0.73 -0.6
35,514 - CCNA_03912 0.75 +0.4
35,535 + CCNA_03912 0.79 -0.6
35,558 + CCNA_03912 0.82 +2.4
35,558 + CCNA_03912 0.82 +0.0
35,585 + CCNA_03912 0.87 +0.8
35,592 + CCNA_03912 0.88 +1.9
35,593 + CCNA_03912 0.88 +1.2
35,604 + CCNA_03912 0.90 +0.8
35,612 - +0.8
35,612 - +0.1
35,623 - +0.7
35,639 + +2.2
35,658 - -2.1
35,661 + +0.4
35,669 - -0.3

Or see this region's nucleotide sequence