Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS12015 and AZOBR_RS12020 are separated by 20 nucleotides AZOBR_RS12020 and AZOBR_RS12025 are separated by 23 nucleotides AZOBR_RS12025 and AZOBR_RS12030 are separated by 126 nucleotides AZOBR_RS12030 and AZOBR_RS12035 are separated by 36 nucleotides
AZOBR_RS12015: AZOBR_RS12015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 2,525,186 to 2,526,529
_RS12015
AZOBR_RS12020: AZOBR_RS12020 - acetyl-CoA carboxylase, at 2,526,550 to 2,526,999
_RS12020
AZOBR_RS12025: AZOBR_RS12025 - 3-dehydroquinate dehydratase, at 2,527,023 to 2,527,472
_RS12025
AZOBR_RS12030: AZOBR_RS12030 - amino acid ABC transporter substrate-binding protein, at 2,527,599 to 2,528,378
_RS12030
AZOBR_RS12035: AZOBR_RS12035 - hypothetical protein, at 2,528,415 to 2,529,878
_RS12035
Position (kb)
2527
2528 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2527.557 kb on - strand at 2527.747 kb on + strand, within AZOBR_RS12030 at 2528.020 kb on + strand, within AZOBR_RS12030 at 2528.020 kb on + strand, within AZOBR_RS12030 at 2528.020 kb on + strand, within AZOBR_RS12030 at 2528.020 kb on + strand, within AZOBR_RS12030 at 2528.020 kb on + strand, within AZOBR_RS12030 at 2528.021 kb on - strand, within AZOBR_RS12030 at 2528.093 kb on - strand, within AZOBR_RS12030
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,527,557 - +1.2 2,527,747 + AZOBR_RS12030 0.19 -0.9 2,528,020 + AZOBR_RS12030 0.54 -0.1 2,528,020 + AZOBR_RS12030 0.54 -0.5 2,528,020 + AZOBR_RS12030 0.54 -0.8 2,528,020 + AZOBR_RS12030 0.54 -0.9 2,528,020 + AZOBR_RS12030 0.54 -4.9 2,528,021 - AZOBR_RS12030 0.54 -0.3 2,528,093 - AZOBR_RS12030 0.63 +0.0
Or see this region's nucleotide sequence