Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS12005 and AZOBR_RS12010 are separated by 125 nucleotides AZOBR_RS12010 and AZOBR_RS12015 are separated by 220 nucleotides AZOBR_RS12015 and AZOBR_RS12020 are separated by 20 nucleotides AZOBR_RS12020 and AZOBR_RS12025 are separated by 23 nucleotides
AZOBR_RS12005: AZOBR_RS12005 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,523,545 to 2,524,186
_RS12005
AZOBR_RS12010: AZOBR_RS12010 - ribonuclease T, at 2,524,312 to 2,524,965
_RS12010
AZOBR_RS12015: AZOBR_RS12015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 2,525,186 to 2,526,529
_RS12015
AZOBR_RS12020: AZOBR_RS12020 - acetyl-CoA carboxylase, at 2,526,550 to 2,526,999
_RS12020
AZOBR_RS12025: AZOBR_RS12025 - 3-dehydroquinate dehydratase, at 2,527,023 to 2,527,472
_RS12025
Position (kb)
2525
2526
2527 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2524.196 kb on + strand at 2524.197 kb on + strand at 2524.197 kb on - strand at 2524.228 kb on + strand at 2524.229 kb on - strand at 2524.415 kb on - strand, within AZOBR_RS12010 at 2524.415 kb on - strand, within AZOBR_RS12010 at 2524.415 kb on - strand, within AZOBR_RS12010 at 2524.415 kb on - strand, within AZOBR_RS12010 at 2524.417 kb on + strand, within AZOBR_RS12010 at 2524.417 kb on + strand, within AZOBR_RS12010 at 2524.418 kb on - strand, within AZOBR_RS12010 at 2524.418 kb on - strand, within AZOBR_RS12010 at 2524.538 kb on - strand, within AZOBR_RS12010 at 2524.538 kb on - strand, within AZOBR_RS12010 at 2524.538 kb on - strand, within AZOBR_RS12010 at 2525.187 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,524,196 + +0.8 2,524,197 + -0.2 2,524,197 - -0.5 2,524,228 + -3.2 2,524,229 - +0.5 2,524,415 - AZOBR_RS12010 0.16 -1.3 2,524,415 - AZOBR_RS12010 0.16 +4.1 2,524,415 - AZOBR_RS12010 0.16 -1.3 2,524,415 - AZOBR_RS12010 0.16 +2.5 2,524,417 + AZOBR_RS12010 0.16 +0.2 2,524,417 + AZOBR_RS12010 0.16 +1.8 2,524,418 - AZOBR_RS12010 0.16 -1.5 2,524,418 - AZOBR_RS12010 0.16 +1.0 2,524,538 - AZOBR_RS12010 0.35 +0.7 2,524,538 - AZOBR_RS12010 0.35 +0.6 2,524,538 - AZOBR_RS12010 0.35 -0.5 2,525,187 + +1.2
Or see this region's nucleotide sequence