Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS05735 and AZOBR_RS05740 are separated by 285 nucleotides AZOBR_RS05740 and AZOBR_RS05745 are separated by 6 nucleotides AZOBR_RS05745 and AZOBR_RS05750 are separated by 197 nucleotides AZOBR_RS05750 and AZOBR_RS05755 are separated by 306 nucleotides
AZOBR_RS05735: AZOBR_RS05735 - 2-hydroxy-acid oxidase, at 1,215,711 to 1,217,057
_RS05735
AZOBR_RS05740: AZOBR_RS05740 - phosphatidylcholine synthase, at 1,217,343 to 1,218,038
_RS05740
AZOBR_RS05745: AZOBR_RS05745 - conserved exported protein of unknown function, at 1,218,045 to 1,218,320
_RS05745
AZOBR_RS05750: AZOBR_RS05750 - hypothetical protein, at 1,218,518 to 1,218,895
_RS05750
AZOBR_RS05755: AZOBR_RS05755 - Butyryl-CoA dehydrogenase (EC 1.3.8.1) (from data) , at 1,219,202 to 1,221,001
_RS05755
Position (kb)
1218
1219 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1217.055 kb on + strand at 1217.055 kb on + strand at 1217.268 kb on + strand at 1217.269 kb on - strand at 1217.571 kb on + strand, within AZOBR_RS05740 at 1217.571 kb on + strand, within AZOBR_RS05740 at 1217.572 kb on - strand, within AZOBR_RS05740 at 1217.580 kb on + strand, within AZOBR_RS05740 at 1217.580 kb on + strand, within AZOBR_RS05740 at 1217.581 kb on - strand, within AZOBR_RS05740 at 1217.581 kb on - strand, within AZOBR_RS05740 at 1217.581 kb on - strand, within AZOBR_RS05740 at 1217.581 kb on - strand, within AZOBR_RS05740 at 1217.581 kb on - strand, within AZOBR_RS05740 at 1217.608 kb on - strand, within AZOBR_RS05740 at 1217.676 kb on + strand, within AZOBR_RS05740 at 1217.677 kb on - strand, within AZOBR_RS05740 at 1217.715 kb on + strand, within AZOBR_RS05740 at 1217.715 kb on + strand, within AZOBR_RS05740 at 1217.715 kb on + strand, within AZOBR_RS05740 at 1217.715 kb on + strand, within AZOBR_RS05740 at 1217.716 kb on - strand, within AZOBR_RS05740 at 1217.716 kb on - strand, within AZOBR_RS05740 at 1217.757 kb on + strand, within AZOBR_RS05740 at 1217.757 kb on + strand, within AZOBR_RS05740 at 1217.758 kb on - strand, within AZOBR_RS05740 at 1217.985 kb on + strand at 1217.988 kb on + strand at 1217.988 kb on + strand at 1217.989 kb on - strand at 1218.036 kb on + strand at 1218.603 kb on + strand, within AZOBR_RS05750 at 1218.603 kb on + strand, within AZOBR_RS05750 at 1218.618 kb on + strand, within AZOBR_RS05750 at 1218.738 kb on + strand, within AZOBR_RS05750 at 1218.739 kb on - strand, within AZOBR_RS05750 at 1218.739 kb on - strand, within AZOBR_RS05750
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,217,055 + -2.6 1,217,055 + +0.7 1,217,268 + -1.3 1,217,269 - -1.7 1,217,571 + AZOBR_RS05740 0.33 -0.5 1,217,571 + AZOBR_RS05740 0.33 +0.5 1,217,572 - AZOBR_RS05740 0.33 -0.0 1,217,580 + AZOBR_RS05740 0.34 +0.1 1,217,580 + AZOBR_RS05740 0.34 -0.4 1,217,581 - AZOBR_RS05740 0.34 +1.3 1,217,581 - AZOBR_RS05740 0.34 +0.4 1,217,581 - AZOBR_RS05740 0.34 +0.8 1,217,581 - AZOBR_RS05740 0.34 -2.7 1,217,581 - AZOBR_RS05740 0.34 +0.0 1,217,608 - AZOBR_RS05740 0.38 -0.4 1,217,676 + AZOBR_RS05740 0.48 +1.3 1,217,677 - AZOBR_RS05740 0.48 -1.9 1,217,715 + AZOBR_RS05740 0.53 -2.1 1,217,715 + AZOBR_RS05740 0.53 -0.0 1,217,715 + AZOBR_RS05740 0.53 +0.2 1,217,715 + AZOBR_RS05740 0.53 +1.1 1,217,716 - AZOBR_RS05740 0.54 -2.4 1,217,716 - AZOBR_RS05740 0.54 -0.2 1,217,757 + AZOBR_RS05740 0.59 -1.9 1,217,757 + AZOBR_RS05740 0.59 -1.9 1,217,758 - AZOBR_RS05740 0.60 -2.4 1,217,985 + -1.4 1,217,988 + +0.5 1,217,988 + -0.8 1,217,989 - +0.1 1,218,036 + +0.3 1,218,603 + AZOBR_RS05750 0.22 +1.8 1,218,603 + AZOBR_RS05750 0.22 -2.1 1,218,618 + AZOBR_RS05750 0.26 +0.9 1,218,738 + AZOBR_RS05750 0.58 -0.0 1,218,739 - AZOBR_RS05750 0.58 -1.3 1,218,739 - AZOBR_RS05750 0.58 +1.0
Or see this region's nucleotide sequence