Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0122 and Shew_3725 are separated by 560 nucleotides Shew_3725 and Shew_3726 are separated by 134 nucleotides Shew_3726 and Shew_3727 are separated by 38 nucleotides
Shew_R0122: Shew_R0122 - 16S ribosomal RNA (RefSeq), at 4,438,044 to 4,439,600
_R0122
Shew_3725: Shew_3725 - carbonic anhydrase (RefSeq), at 4,440,161 to 4,440,718
_3725
Shew_3726: Shew_3726 - hypothetical protein (RefSeq), at 4,440,853 to 4,441,125
_3726
Shew_3727: Shew_3727 - shikimate 5-dehydrogenase (RefSeq), at 4,441,164 to 4,442,078
_3727
Position (kb) 4440
4441 Strain fitness (log2 ratio) -1
0
1 at 4440.580 kb on + strand, within Shew_3725 at 4440.580 kb on + strand, within Shew_3725 at 4441.078 kb on - strand, within Shew_3726 at 4441.220 kb on + strand at 4441.220 kb on + strand at 4441.228 kb on - strand at 4441.228 kb on - strand at 4441.245 kb on + strand at 4441.341 kb on + strand, within Shew_3727 at 4441.347 kb on + strand, within Shew_3727 at 4441.427 kb on - strand, within Shew_3727 at 4441.434 kb on + strand, within Shew_3727 at 4441.471 kb on + strand, within Shew_3727 at 4441.575 kb on - strand, within Shew_3727 at 4441.681 kb on - strand, within Shew_3727 at 4441.681 kb on - strand, within Shew_3727 at 4441.681 kb on - strand, within Shew_3727
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 4,440,580 + Shew_3725 0.75 -0.2 4,440,580 + Shew_3725 0.75 -0.6 4,441,078 - Shew_3726 0.82 -0.1 4,441,220 + +0.1 4,441,220 + +0.4 4,441,228 - +0.9 4,441,228 - +0.3 4,441,245 + -1.6 4,441,341 + Shew_3727 0.19 +0.2 4,441,347 + Shew_3727 0.20 -1.5 4,441,427 - Shew_3727 0.29 +1.0 4,441,434 + Shew_3727 0.30 -0.0 4,441,471 + Shew_3727 0.34 +0.3 4,441,575 - Shew_3727 0.45 -0.7 4,441,681 - Shew_3727 0.57 +0.5 4,441,681 - Shew_3727 0.57 +0.3 4,441,681 - Shew_3727 0.57 -0.0
Or see this region's nucleotide sequence