Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3521 and Shew_3522 overlap by 10 nucleotides Shew_3522 and Shew_3523 are separated by 32 nucleotides Shew_3523 and Shew_3524 overlap by 1 nucleotides Shew_3524 and fabG overlap by 4 nucleotides
Shew_3521: Shew_3521 - tryptophan halogenase (RefSeq), at 4,208,445 to 4,209,719
_3521
Shew_3522: Shew_3522 - hypothetical protein (RefSeq), at 4,209,710 to 4,210,282
_3522
Shew_3523: Shew_3523 - 3-oxoacyl-(acyl carrier protein) synthase I (RefSeq), at 4,210,315 to 4,211,520
_3523
Shew_3524: Shew_3524 - Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase, FabA/FabZ (RefSeq), at 4,211,520 to 4,212,113
_3524
Shew_3525: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 4,212,110 to 4,212,835
fabG
Position (kb)
4210
4211
4212 Strain fitness (log2 ratio)
-1
0
1 at 4209.411 kb on + strand, within Shew_3521 at 4209.615 kb on - strand at 4209.638 kb on - strand at 4209.809 kb on + strand, within Shew_3522 at 4209.876 kb on + strand, within Shew_3522 at 4210.277 kb on + strand at 4210.477 kb on - strand, within Shew_3523 at 4210.604 kb on + strand, within Shew_3523 at 4210.716 kb on + strand, within Shew_3523 at 4210.984 kb on + strand, within Shew_3523 at 4211.150 kb on + strand, within Shew_3523 at 4211.263 kb on + strand, within Shew_3523 at 4211.578 kb on - strand at 4211.681 kb on - strand, within Shew_3524 at 4211.778 kb on + strand, within Shew_3524 at 4211.786 kb on - strand, within Shew_3524 at 4212.026 kb on + strand, within Shew_3524 at 4212.173 kb on - strand at 4212.173 kb on - strand at 4212.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 4,209,411 + Shew_3521 0.76 +0.6 4,209,615 - -0.6 4,209,638 - -0.5 4,209,809 + Shew_3522 0.17 +0.8 4,209,876 + Shew_3522 0.29 -0.4 4,210,277 + -0.6 4,210,477 - Shew_3523 0.13 +0.1 4,210,604 + Shew_3523 0.24 -0.6 4,210,716 + Shew_3523 0.33 -0.4 4,210,984 + Shew_3523 0.55 -0.5 4,211,150 + Shew_3523 0.69 -0.2 4,211,263 + Shew_3523 0.79 -0.5 4,211,578 - -0.7 4,211,681 - Shew_3524 0.27 +0.3 4,211,778 + Shew_3524 0.43 -0.0 4,211,786 - Shew_3524 0.45 +0.2 4,212,026 + Shew_3524 0.85 -0.4 4,212,173 - -0.1 4,212,173 - +1.5 4,212,173 - -0.2
Or see this region's nucleotide sequence