Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1118 and Shew_1119 are separated by 261 nucleotides Shew_1119 and Shew_R0024 are separated by 186 nucleotides Shew_R0024 and Shew_R0025 are separated by 7 nucleotides Shew_R0025 and Shew_1120 are separated by 386 nucleotides
Shew_1118: Shew_1118 - hypothetical protein (RefSeq), at 1,293,141 to 1,294,304
_1118
Shew_1119: Shew_1119 - glucose/galactose transporter (RefSeq), at 1,294,566 to 1,295,822
_1119
Shew_R0024: Shew_R0024 - tRNA-Thr (RefSeq), at 1,296,009 to 1,296,084
_R0024
Shew_R0025: Shew_R0025 - tRNA-Phe (RefSeq), at 1,296,092 to 1,296,167
_R0025
Shew_1120: Shew_1120 - cytochrome B561 (RefSeq), at 1,296,554 to 1,297,108
_1120
Position (kb)
1294
1295
1296 Strain fitness (log2 ratio)
-1
0
1 at 1293.940 kb on + strand, within Shew_1118 at 1293.953 kb on - strand, within Shew_1118 at 1294.350 kb on + strand at 1294.665 kb on + strand at 1294.726 kb on + strand, within Shew_1119 at 1294.880 kb on - strand, within Shew_1119 at 1295.023 kb on + strand, within Shew_1119 at 1295.353 kb on - strand, within Shew_1119 at 1295.489 kb on + strand, within Shew_1119 at 1295.790 kb on + strand at 1295.805 kb on - strand at 1295.812 kb on + strand at 1296.731 kb on + strand, within Shew_1120 at 1296.731 kb on + strand, within Shew_1120 at 1296.731 kb on + strand, within Shew_1120 at 1296.739 kb on - strand, within Shew_1120
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,293,940 + Shew_1118 0.69 +0.1 1,293,953 - Shew_1118 0.70 +0.0 1,294,350 + +0.4 1,294,665 + -0.0 1,294,726 + Shew_1119 0.13 +0.0 1,294,880 - Shew_1119 0.25 -0.2 1,295,023 + Shew_1119 0.36 -1.1 1,295,353 - Shew_1119 0.63 +0.5 1,295,489 + Shew_1119 0.73 -0.7 1,295,790 + +0.4 1,295,805 - -0.1 1,295,812 + -0.4 1,296,731 + Shew_1120 0.32 +0.3 1,296,731 + Shew_1120 0.32 +0.1 1,296,731 + Shew_1120 0.32 +0.3 1,296,739 - Shew_1120 0.33 +0.8
Or see this region's nucleotide sequence