Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4424 and BT4425 are separated by 39 nucleotides BT4425 and BT4426 are separated by 44 nucleotides BT4426 and BT4427 overlap by 1 nucleotides
BT4424: BT4424 - sorbitol dehydrogenase (NCBI ptt file), at 5,827,129 to 5,828,163
BT4424
BT4425: BT4425 - deoxyribose-phosphate aldolase (NCBI ptt file), at 5,828,203 to 5,828,919
BT4425
BT4426: BT4426 - conserved hypothetical protein (NCBI ptt file), at 5,828,964 to 5,831,498
BT4426
BT4427: BT4427 - putative surface layer protein, with cytochrome c domain (NCBI ptt file), at 5,831,498 to 5,833,297
BT4427
Position (kb)
5828
5829
5830
5831
5832 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5828.169 kb on - strand at 5828.176 kb on - strand at 5828.185 kb on - strand at 5828.185 kb on - strand at 5828.274 kb on + strand at 5828.451 kb on + strand, within BT4425 at 5828.638 kb on + strand, within BT4425 at 5828.863 kb on - strand at 5828.886 kb on - strand at 5828.893 kb on + strand at 5828.938 kb on - strand at 5828.938 kb on - strand at 5828.938 kb on - strand at 5829.007 kb on + strand at 5829.007 kb on + strand at 5829.084 kb on - strand at 5829.125 kb on - strand at 5829.173 kb on + strand at 5829.211 kb on - strand at 5829.330 kb on - strand, within BT4426 at 5829.617 kb on - strand, within BT4426 at 5829.702 kb on + strand, within BT4426 at 5829.797 kb on - strand, within BT4426 at 5829.858 kb on - strand, within BT4426 at 5829.858 kb on - strand, within BT4426 at 5829.887 kb on + strand, within BT4426 at 5829.898 kb on + strand, within BT4426 at 5829.898 kb on + strand, within BT4426 at 5829.899 kb on - strand, within BT4426 at 5829.918 kb on - strand, within BT4426 at 5829.958 kb on - strand, within BT4426 at 5829.975 kb on - strand, within BT4426 at 5830.110 kb on - strand, within BT4426 at 5830.167 kb on - strand, within BT4426 at 5830.167 kb on - strand, within BT4426 at 5830.244 kb on + strand, within BT4426 at 5830.254 kb on + strand, within BT4426 at 5830.255 kb on - strand, within BT4426 at 5830.266 kb on - strand, within BT4426 at 5830.323 kb on - strand, within BT4426 at 5830.489 kb on - strand, within BT4426 at 5830.490 kb on + strand, within BT4426 at 5830.513 kb on - strand, within BT4426 at 5830.513 kb on - strand, within BT4426 at 5830.721 kb on - strand, within BT4426 at 5830.721 kb on - strand, within BT4426 at 5831.109 kb on + strand, within BT4426 at 5831.227 kb on - strand, within BT4426 at 5831.249 kb on + strand at 5831.400 kb on + strand at 5831.464 kb on - strand at 5831.514 kb on + strand at 5831.525 kb on - strand at 5831.534 kb on - strand at 5831.774 kb on + strand, within BT4427 at 5831.827 kb on + strand, within BT4427 at 5831.840 kb on - strand, within BT4427 at 5831.846 kb on + strand at 5832.041 kb on - strand, within BT4427 at 5832.156 kb on - strand, within BT4427 at 5832.344 kb on - strand, within BT4427 at 5832.344 kb on - strand, within BT4427 at 5832.358 kb on - strand, within BT4427 at 5832.388 kb on + strand, within BT4427 at 5832.389 kb on - strand, within BT4427 at 5832.389 kb on - strand, within BT4427 at 5832.496 kb on - strand, within BT4427 at 5832.496 kb on - strand, within BT4427 at 5832.496 kb on - strand, within BT4427 at 5832.497 kb on + strand, within BT4427 at 5832.498 kb on - strand, within BT4427 at 5832.498 kb on - strand, within BT4427
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 5,828,169 - -1.9 5,828,176 - +1.0 5,828,185 - -1.1 5,828,185 - +0.5 5,828,274 + +0.7 5,828,451 + BT4425 0.35 -1.6 5,828,638 + BT4425 0.61 +0.2 5,828,863 - +0.1 5,828,886 - -2.1 5,828,893 + +0.4 5,828,938 - +0.8 5,828,938 - -0.2 5,828,938 - -0.6 5,829,007 + -2.1 5,829,007 + -2.2 5,829,084 - -0.2 5,829,125 - -0.5 5,829,173 + +1.4 5,829,211 - +2.4 5,829,330 - BT4426 0.14 -1.4 5,829,617 - BT4426 0.26 -2.3 5,829,702 + BT4426 0.29 -1.2 5,829,797 - BT4426 0.33 +0.1 5,829,858 - BT4426 0.35 -0.6 5,829,858 - BT4426 0.35 +0.9 5,829,887 + BT4426 0.36 -1.2 5,829,898 + BT4426 0.37 -1.9 5,829,898 + BT4426 0.37 -1.8 5,829,899 - BT4426 0.37 +0.5 5,829,918 - BT4426 0.38 -2.3 5,829,958 - BT4426 0.39 -1.2 5,829,975 - BT4426 0.40 -1.6 5,830,110 - BT4426 0.45 -0.1 5,830,167 - BT4426 0.47 -4.2 5,830,167 - BT4426 0.47 -0.9 5,830,244 + BT4426 0.50 +1.1 5,830,254 + BT4426 0.51 -1.3 5,830,255 - BT4426 0.51 -0.4 5,830,266 - BT4426 0.51 -2.5 5,830,323 - BT4426 0.54 -1.0 5,830,489 - BT4426 0.60 -2.3 5,830,490 + BT4426 0.60 -0.6 5,830,513 - BT4426 0.61 -2.0 5,830,513 - BT4426 0.61 +0.4 5,830,721 - BT4426 0.69 -2.0 5,830,721 - BT4426 0.69 +0.4 5,831,109 + BT4426 0.85 +1.4 5,831,227 - BT4426 0.89 -1.6 5,831,249 + +1.4 5,831,400 + +1.4 5,831,464 - +0.2 5,831,514 + +1.0 5,831,525 - +0.1 5,831,534 - -1.7 5,831,774 + BT4427 0.15 -2.3 5,831,827 + BT4427 0.18 -0.3 5,831,840 - BT4427 0.19 +1.5 5,831,846 + +0.4 5,832,041 - BT4427 0.30 -1.3 5,832,156 - BT4427 0.37 -2.8 5,832,344 - BT4427 0.47 +0.4 5,832,344 - BT4427 0.47 -2.4 5,832,358 - BT4427 0.48 -2.1 5,832,388 + BT4427 0.49 +0.1 5,832,389 - BT4427 0.49 +0.0 5,832,389 - BT4427 0.49 -1.8 5,832,496 - BT4427 0.55 -1.2 5,832,496 - BT4427 0.55 +0.4 5,832,496 - BT4427 0.55 -1.1 5,832,497 + BT4427 0.56 -0.9 5,832,498 - BT4427 0.56 -0.2 5,832,498 - BT4427 0.56 +1.2
Or see this region's nucleotide sequence