Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4188 and BT4189 are separated by 6 nucleotides BT4189 and BT4190 are separated by 40 nucleotides BT4190 and BT4191 overlap by 35 nucleotides
BT4188: BT4188 - enoyl-[acyl-carrier-protein] reductase (NCBI ptt file), at 5,523,365 to 5,524,222
BT4188
BT4189: BT4189 - conserved hypothetical protein (NCBI ptt file), at 5,524,229 to 5,525,236
BT4189
BT4190: BT4190 - putative S-adenosylmethionine-dependent methytransferase (NCBI ptt file), at 5,525,277 to 5,525,981
BT4190
BT4191: BT4191 - hypothetical protein (NCBI ptt file), at 5,525,947 to 5,527,068
BT4191
Position (kb)
5524
5525
5526 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5523.266 kb on + strand at 5523.266 kb on + strand at 5523.286 kb on + strand at 5523.287 kb on - strand at 5523.295 kb on + strand at 5523.330 kb on + strand at 5524.207 kb on + strand at 5524.208 kb on - strand at 5524.252 kb on + strand at 5524.252 kb on + strand at 5524.252 kb on + strand at 5524.258 kb on - strand at 5524.260 kb on + strand at 5524.261 kb on - strand at 5524.263 kb on + strand at 5524.263 kb on + strand at 5524.267 kb on + strand at 5524.268 kb on - strand at 5524.344 kb on - strand, within BT4189 at 5524.344 kb on - strand, within BT4189 at 5524.365 kb on - strand, within BT4189 at 5524.365 kb on - strand, within BT4189 at 5524.365 kb on - strand, within BT4189 at 5524.446 kb on + strand, within BT4189 at 5524.454 kb on - strand, within BT4189 at 5524.540 kb on - strand at 5524.540 kb on - strand, within BT4189 at 5524.540 kb on - strand, within BT4189 at 5524.630 kb on - strand, within BT4189 at 5524.684 kb on + strand, within BT4189 at 5524.684 kb on + strand, within BT4189 at 5524.685 kb on - strand, within BT4189 at 5524.712 kb on + strand, within BT4189 at 5524.712 kb on + strand, within BT4189 at 5524.714 kb on + strand, within BT4189 at 5524.715 kb on - strand, within BT4189 at 5524.751 kb on + strand, within BT4189 at 5524.751 kb on + strand, within BT4189 at 5524.850 kb on - strand, within BT4189 at 5524.850 kb on - strand, within BT4189 at 5524.997 kb on + strand, within BT4189 at 5524.998 kb on - strand, within BT4189 at 5524.999 kb on + strand, within BT4189 at 5524.999 kb on + strand, within BT4189 at 5524.999 kb on + strand, within BT4189 at 5525.000 kb on - strand, within BT4189 at 5525.134 kb on - strand, within BT4189 at 5525.141 kb on + strand at 5525.155 kb on + strand at 5525.188 kb on + strand at 5525.188 kb on + strand at 5525.203 kb on + strand at 5525.204 kb on - strand at 5525.246 kb on - strand at 5525.248 kb on + strand at 5525.248 kb on + strand at 5525.255 kb on + strand at 5525.259 kb on + strand at 5525.266 kb on + strand at 5525.293 kb on - strand at 5525.338 kb on + strand at 5525.338 kb on + strand at 5525.338 kb on + strand at 5525.346 kb on + strand at 5525.346 kb on + strand at 5525.348 kb on + strand, within BT4190 at 5525.361 kb on - strand, within BT4190 at 5525.478 kb on - strand, within BT4190 at 5525.478 kb on - strand, within BT4190 at 5525.493 kb on - strand, within BT4190 at 5525.493 kb on - strand, within BT4190 at 5525.502 kb on + strand at 5525.714 kb on - strand, within BT4190 at 5525.732 kb on + strand, within BT4190 at 5525.785 kb on + strand, within BT4190 at 5525.785 kb on + strand at 5525.813 kb on + strand, within BT4190 at 5525.822 kb on - strand, within BT4190 at 5525.822 kb on - strand, within BT4190 at 5525.828 kb on + strand, within BT4190 at 5525.861 kb on - strand, within BT4190 at 5525.864 kb on + strand, within BT4190 at 5525.864 kb on + strand, within BT4190 at 5525.865 kb on - strand, within BT4190 at 5525.865 kb on - strand, within BT4190 at 5525.876 kb on + strand, within BT4190 at 5525.900 kb on - strand, within BT4190 at 5525.934 kb on - strand at 5525.972 kb on - strand at 5525.974 kb on - strand at 5525.980 kb on - strand at 5525.999 kb on + strand at 5526.073 kb on - strand, within BT4191 at 5526.073 kb on - strand, within BT4191 at 5526.102 kb on + strand, within BT4191
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 5,523,266 + +0.3 5,523,266 + +0.8 5,523,286 + -1.6 5,523,287 - -1.4 5,523,295 + -3.9 5,523,330 + -2.5 5,524,207 + -0.4 5,524,208 - -0.6 5,524,252 + -1.4 5,524,252 + -2.2 5,524,252 + -2.7 5,524,258 - -0.2 5,524,260 + -1.8 5,524,261 - -2.3 5,524,263 + -1.6 5,524,263 + -0.6 5,524,267 + -0.7 5,524,268 - -2.3 5,524,344 - BT4189 0.11 -2.3 5,524,344 - BT4189 0.11 -1.9 5,524,365 - BT4189 0.13 -3.0 5,524,365 - BT4189 0.13 -2.6 5,524,365 - BT4189 0.13 -2.3 5,524,446 + BT4189 0.22 -1.7 5,524,454 - BT4189 0.22 +0.8 5,524,540 - +1.4 5,524,540 - BT4189 0.31 +0.0 5,524,540 - BT4189 0.31 -2.2 5,524,630 - BT4189 0.40 -1.2 5,524,684 + BT4189 0.45 -1.1 5,524,684 + BT4189 0.45 -1.4 5,524,685 - BT4189 0.45 -2.2 5,524,712 + BT4189 0.48 -1.0 5,524,712 + BT4189 0.48 +0.8 5,524,714 + BT4189 0.48 -3.1 5,524,715 - BT4189 0.48 -3.0 5,524,751 + BT4189 0.52 -2.5 5,524,751 + BT4189 0.52 +0.0 5,524,850 - BT4189 0.62 +1.4 5,524,850 - BT4189 0.62 +1.4 5,524,997 + BT4189 0.76 -1.2 5,524,998 - BT4189 0.76 -0.8 5,524,999 + BT4189 0.76 -1.7 5,524,999 + BT4189 0.76 -0.7 5,524,999 + BT4189 0.76 -4.8 5,525,000 - BT4189 0.76 -3.3 5,525,134 - BT4189 0.90 -1.6 5,525,141 + -2.3 5,525,155 + -2.5 5,525,188 + -1.6 5,525,188 + -2.8 5,525,203 + -2.1 5,525,204 - -0.2 5,525,246 - -1.4 5,525,248 + +2.4 5,525,248 + +0.1 5,525,255 + -1.6 5,525,259 + +1.8 5,525,266 + -0.3 5,525,293 - -0.9 5,525,338 + +0.9 5,525,338 + +1.2 5,525,338 + +0.7 5,525,346 + +2.2 5,525,346 + +0.3 5,525,348 + BT4190 0.10 -0.2 5,525,361 - BT4190 0.12 -1.6 5,525,478 - BT4190 0.29 -0.6 5,525,478 - BT4190 0.29 -1.7 5,525,493 - BT4190 0.31 +0.8 5,525,493 - BT4190 0.31 -2.4 5,525,502 + +0.4 5,525,714 - BT4190 0.62 -3.2 5,525,732 + BT4190 0.65 +1.1 5,525,785 + BT4190 0.72 +0.2 5,525,785 + +0.7 5,525,813 + BT4190 0.76 -0.1 5,525,822 - BT4190 0.77 -0.8 5,525,822 - BT4190 0.77 -0.6 5,525,828 + BT4190 0.78 +0.1 5,525,861 - BT4190 0.83 -1.3 5,525,864 + BT4190 0.83 -0.1 5,525,864 + BT4190 0.83 -0.0 5,525,865 - BT4190 0.83 +3.4 5,525,865 - BT4190 0.83 -0.8 5,525,876 + BT4190 0.85 -0.1 5,525,900 - BT4190 0.88 -0.2 5,525,934 - -0.3 5,525,972 - -0.7 5,525,974 - -0.9 5,525,980 - +0.4 5,525,999 + -0.4 5,526,073 - BT4191 0.11 +0.4 5,526,073 - BT4191 0.11 -1.3 5,526,102 + BT4191 0.14 -1.5
Or see this region's nucleotide sequence