Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2160 and BT2161 are separated by 41 nucleotides BT2161 and BT2162 are separated by 14 nucleotides BT2162 and BT2163 are separated by 2 nucleotides BT2163 and BT2164 are separated by 160 nucleotides
BT2160: BT2160 - SusR4: regulator of disaccharide catabolism (SusR4) (from data) , at 2,719,301 to 2,720,986
BT2160
BT2161: BT2161 - 50S ribosomal protein L9 (NCBI ptt file), at 2,721,028 to 2,721,471
BT2161
BT2162: BT2162 - 30S ribosomal protein S18 (NCBI ptt file), at 2,721,486 to 2,721,758
BT2162
BT2163: BT2163 - 30S ribosomal protein S6 (NCBI ptt file), at 2,721,761 to 2,722,105
BT2163
BT2164: BT2164 - MarR family transcriptional regulator (NCBI ptt file), at 2,722,266 to 2,722,712
BT2164
Position (kb)
2721
2722 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2720.115 kb on + strand, within BT2160 at 2720.116 kb on - strand, within BT2160 at 2720.116 kb on - strand, within BT2160 at 2720.116 kb on - strand, within BT2160 at 2720.191 kb on + strand, within BT2160 at 2720.191 kb on + strand, within BT2160 at 2720.191 kb on + strand, within BT2160 at 2720.192 kb on - strand, within BT2160 at 2720.245 kb on - strand, within BT2160 at 2720.254 kb on + strand, within BT2160 at 2720.255 kb on - strand, within BT2160 at 2720.265 kb on + strand, within BT2160 at 2720.269 kb on + strand, within BT2160 at 2720.269 kb on + strand, within BT2160 at 2720.279 kb on + strand, within BT2160 at 2720.343 kb on + strand, within BT2160 at 2720.349 kb on + strand, within BT2160 at 2720.350 kb on - strand, within BT2160 at 2720.365 kb on + strand, within BT2160 at 2720.381 kb on - strand, within BT2160 at 2720.398 kb on - strand, within BT2160 at 2720.463 kb on + strand, within BT2160 at 2720.482 kb on - strand, within BT2160 at 2720.482 kb on - strand, within BT2160 at 2720.485 kb on + strand, within BT2160 at 2720.493 kb on + strand, within BT2160 at 2720.494 kb on - strand, within BT2160 at 2720.561 kb on - strand, within BT2160 at 2720.563 kb on - strand, within BT2160 at 2720.582 kb on + strand, within BT2160 at 2720.583 kb on - strand, within BT2160 at 2720.583 kb on - strand, within BT2160 at 2720.583 kb on - strand, within BT2160 at 2720.664 kb on + strand, within BT2160 at 2720.665 kb on - strand, within BT2160 at 2720.665 kb on - strand, within BT2160 at 2720.681 kb on + strand, within BT2160 at 2720.682 kb on - strand, within BT2160 at 2720.682 kb on - strand, within BT2160 at 2720.683 kb on + strand, within BT2160 at 2720.683 kb on + strand, within BT2160 at 2720.695 kb on + strand, within BT2160 at 2720.697 kb on + strand, within BT2160 at 2720.725 kb on + strand, within BT2160 at 2720.725 kb on + strand, within BT2160 at 2720.725 kb on + strand, within BT2160 at 2720.726 kb on - strand, within BT2160 at 2720.822 kb on + strand at 2720.871 kb on + strand at 2720.872 kb on - strand at 2720.928 kb on + strand at 2720.929 kb on - strand at 2720.950 kb on + strand at 2720.951 kb on - strand at 2720.953 kb on + strand at 2720.953 kb on + strand at 2720.965 kb on + strand at 2720.981 kb on - strand at 2721.009 kb on - strand at 2721.011 kb on - strand at 2721.018 kb on - strand at 2721.041 kb on - strand at 2721.208 kb on - strand, within BT2161 at 2721.211 kb on - strand, within BT2161 at 2721.364 kb on - strand, within BT2161 at 2721.430 kb on - strand at 2721.430 kb on - strand at 2721.443 kb on - strand at 2721.505 kb on - strand at 2721.505 kb on - strand at 2721.509 kb on - strand at 2722.206 kb on + strand at 2722.211 kb on + strand at 2722.299 kb on - strand at 2722.300 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 2,720,115 + BT2160 0.48 -2.3 2,720,116 - BT2160 0.48 -2.1 2,720,116 - BT2160 0.48 +0.9 2,720,116 - BT2160 0.48 +2.1 2,720,191 + BT2160 0.53 -0.5 2,720,191 + BT2160 0.53 +0.4 2,720,191 + BT2160 0.53 -0.2 2,720,192 - BT2160 0.53 +1.4 2,720,245 - BT2160 0.56 -1.8 2,720,254 + BT2160 0.57 +1.8 2,720,255 - BT2160 0.57 +1.4 2,720,265 + BT2160 0.57 -1.6 2,720,269 + BT2160 0.57 -0.4 2,720,269 + BT2160 0.57 -1.2 2,720,279 + BT2160 0.58 -0.4 2,720,343 + BT2160 0.62 -1.4 2,720,349 + BT2160 0.62 -1.7 2,720,350 - BT2160 0.62 +0.8 2,720,365 + BT2160 0.63 -1.6 2,720,381 - BT2160 0.64 -0.5 2,720,398 - BT2160 0.65 +0.4 2,720,463 + BT2160 0.69 -1.0 2,720,482 - BT2160 0.70 -0.2 2,720,482 - BT2160 0.70 -2.0 2,720,485 + BT2160 0.70 -0.6 2,720,493 + BT2160 0.71 -1.0 2,720,494 - BT2160 0.71 +3.5 2,720,561 - BT2160 0.75 +0.1 2,720,563 - BT2160 0.75 -0.5 2,720,582 + BT2160 0.76 -0.6 2,720,583 - BT2160 0.76 -2.3 2,720,583 - BT2160 0.76 +0.3 2,720,583 - BT2160 0.76 -3.3 2,720,664 + BT2160 0.81 +0.3 2,720,665 - BT2160 0.81 +1.4 2,720,665 - BT2160 0.81 +1.4 2,720,681 + BT2160 0.82 -0.6 2,720,682 - BT2160 0.82 +0.9 2,720,682 - BT2160 0.82 -0.8 2,720,683 + BT2160 0.82 -1.7 2,720,683 + BT2160 0.82 -1.7 2,720,695 + BT2160 0.83 -0.6 2,720,697 + BT2160 0.83 -3.7 2,720,725 + BT2160 0.84 +1.4 2,720,725 + BT2160 0.84 -2.3 2,720,725 + BT2160 0.84 -2.7 2,720,726 - BT2160 0.85 +1.9 2,720,822 + -2.2 2,720,871 + -3.2 2,720,872 - +1.8 2,720,928 + -4.5 2,720,929 - -2.2 2,720,950 + -3.0 2,720,951 - +0.2 2,720,953 + -4.2 2,720,953 + -4.0 2,720,965 + -3.7 2,720,981 - +1.4 2,721,009 - -1.8 2,721,011 - -2.7 2,721,018 - -1.6 2,721,041 - -3.7 2,721,208 - BT2161 0.41 -1.2 2,721,211 - BT2161 0.41 -4.2 2,721,364 - BT2161 0.76 -0.2 2,721,430 - -2.1 2,721,430 - -1.0 2,721,443 - -1.8 2,721,505 - -2.1 2,721,505 - -0.6 2,721,509 - +0.4 2,722,206 + -0.4 2,722,211 + +0.2 2,722,299 - -0.2 2,722,300 + -2.0
Or see this region's nucleotide sequence