Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1729 and BT1730 overlap by 19 nucleotides BT1730 and BT1731 are separated by 0 nucleotides BT1731 and BT1732 are separated by 11 nucleotides
BT1729: BT1729 - peptide chain release factor 3 (RF-3) (NCBI ptt file), at 2,127,615 to 2,129,213
BT1729
BT1730: BT1730 - putative dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 2,129,195 to 2,130,043
BT1730
BT1731: BT1731 - hypothetical protein (NCBI ptt file), at 2,130,044 to 2,130,589
BT1731
BT1732: BT1732 - conserved hypothetical protein (NCBI ptt file), at 2,130,601 to 2,131,254
BT1732
Position (kb)
2129
2130
2131 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2128.214 kb on - strand, within BT1729 at 2128.223 kb on + strand, within BT1729 at 2128.224 kb on - strand, within BT1729 at 2128.224 kb on - strand, within BT1729 at 2128.228 kb on - strand, within BT1729 at 2128.245 kb on + strand, within BT1729 at 2128.246 kb on - strand, within BT1729 at 2128.246 kb on - strand, within BT1729 at 2128.296 kb on - strand, within BT1729 at 2128.301 kb on + strand, within BT1729 at 2128.360 kb on - strand, within BT1729 at 2128.443 kb on + strand, within BT1729 at 2128.575 kb on + strand, within BT1729 at 2128.576 kb on - strand, within BT1729 at 2128.588 kb on - strand, within BT1729 at 2128.621 kb on + strand, within BT1729 at 2128.622 kb on - strand, within BT1729 at 2128.647 kb on - strand, within BT1729 at 2128.649 kb on + strand, within BT1729 at 2128.651 kb on + strand, within BT1729 at 2128.651 kb on + strand, within BT1729 at 2128.682 kb on + strand, within BT1729 at 2128.864 kb on - strand, within BT1729 at 2128.871 kb on + strand, within BT1729 at 2128.881 kb on + strand, within BT1729 at 2128.895 kb on + strand, within BT1729 at 2128.900 kb on + strand, within BT1729 at 2128.901 kb on - strand, within BT1729 at 2128.943 kb on + strand, within BT1729 at 2128.944 kb on - strand, within BT1729 at 2129.051 kb on + strand, within BT1729 at 2129.124 kb on - strand at 2129.145 kb on - strand at 2129.177 kb on + strand at 2129.189 kb on + strand at 2129.194 kb on - strand at 2130.606 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 2,128,214 - BT1729 0.37 -1.5 2,128,223 + BT1729 0.38 -2.1 2,128,224 - BT1729 0.38 +0.5 2,128,224 - BT1729 0.38 +1.3 2,128,228 - BT1729 0.38 -1.5 2,128,245 + BT1729 0.39 -2.1 2,128,246 - BT1729 0.39 -3.3 2,128,246 - BT1729 0.39 -0.3 2,128,296 - BT1729 0.43 -2.1 2,128,301 + BT1729 0.43 -1.6 2,128,360 - BT1729 0.47 -3.1 2,128,443 + BT1729 0.52 -2.2 2,128,575 + BT1729 0.60 -2.7 2,128,576 - BT1729 0.60 -3.9 2,128,588 - BT1729 0.61 -1.5 2,128,621 + BT1729 0.63 -2.3 2,128,622 - BT1729 0.63 -3.1 2,128,647 - BT1729 0.65 -0.7 2,128,649 + BT1729 0.65 -2.9 2,128,651 + BT1729 0.65 -2.5 2,128,651 + BT1729 0.65 -3.7 2,128,682 + BT1729 0.67 -0.3 2,128,864 - BT1729 0.78 -2.2 2,128,871 + BT1729 0.79 -2.2 2,128,881 + BT1729 0.79 -3.1 2,128,895 + BT1729 0.80 -3.9 2,128,900 + BT1729 0.80 -2.4 2,128,901 - BT1729 0.80 -3.6 2,128,943 + BT1729 0.83 -2.3 2,128,944 - BT1729 0.83 -3.9 2,129,051 + BT1729 0.90 -3.2 2,129,124 - -3.4 2,129,145 - -2.9 2,129,177 + -3.2 2,129,189 + -3.3 2,129,194 - +3.3 2,130,606 - -0.7
Or see this region's nucleotide sequence