Experiment: BHIS with Fusidic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0074 and BT0075 are separated by 932 nucleotides BT0075 and BT0076 are separated by 460 nucleotides
BT0074: BT0074 - hypothetical protein (NCBI ptt file), at 71,009 to 71,500
BT0074
BT0075: BT0075 - conserved hypothetical protein (NCBI ptt file), at 72,433 to 72,723
BT0075
BT0076: BT0076 - transposase (NCBI ptt file), at 73,184 to 74,416
BT0076
Position (kb) 72
73 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 71.610 kb on + strand at 71.614 kb on - strand at 71.615 kb on + strand at 71.615 kb on + strand at 71.616 kb on - strand at 71.616 kb on - strand at 71.635 kb on + strand at 71.754 kb on + strand at 71.763 kb on + strand at 71.852 kb on - strand at 71.862 kb on + strand at 71.862 kb on + strand at 71.863 kb on - strand at 71.873 kb on - strand at 71.894 kb on - strand at 71.918 kb on + strand at 71.919 kb on - strand at 71.944 kb on + strand at 71.945 kb on - strand at 71.998 kb on + strand at 72.131 kb on - strand at 72.149 kb on - strand at 72.157 kb on + strand at 72.202 kb on + strand at 72.258 kb on - strand at 72.422 kb on - strand at 72.428 kb on - strand at 72.472 kb on - strand, within BT0075 at 72.705 kb on + strand at 72.989 kb on + strand at 72.990 kb on - strand at 72.990 kb on - strand at 73.267 kb on + strand at 73.268 kb on - strand at 73.322 kb on - strand, within BT0076 at 73.393 kb on - strand, within BT0076 at 73.393 kb on - strand, within BT0076 at 73.411 kb on + strand, within BT0076 at 73.411 kb on - strand, within BT0076 at 73.412 kb on - strand, within BT0076 at 73.412 kb on - strand, within BT0076 at 73.412 kb on - strand, within BT0076 at 73.571 kb on - strand, within BT0076 at 73.629 kb on - strand, within BT0076 at 73.629 kb on - strand, within BT0076 at 73.630 kb on + strand, within BT0076 at 73.631 kb on - strand, within BT0076 at 73.692 kb on - strand, within BT0076 at 73.692 kb on - strand, within BT0076 at 73.703 kb on - strand, within BT0076 at 73.721 kb on - strand, within BT0076
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.004 mg/ml remove 71,610 + -0.2 71,614 - +0.0 71,615 + -0.0 71,615 + -0.7 71,616 - -0.4 71,616 - -1.1 71,635 + +0.4 71,754 + -1.4 71,763 + +0.6 71,852 - -0.7 71,862 + -2.4 71,862 + -0.9 71,863 - -1.3 71,873 - -3.1 71,894 - +0.1 71,918 + +1.2 71,919 - -0.9 71,944 + +0.0 71,945 - +1.2 71,998 + -1.2 72,131 - -2.7 72,149 - -5.5 72,157 + -2.3 72,202 + -0.0 72,258 - +0.2 72,422 - -1.1 72,428 - +1.0 72,472 - BT0075 0.13 -2.0 72,705 + -1.0 72,989 + -2.0 72,990 - +0.1 72,990 - -2.1 73,267 + -2.3 73,268 - -0.6 73,322 - BT0076 0.11 -0.3 73,393 - BT0076 0.17 +1.1 73,393 - BT0076 0.17 -0.3 73,411 + BT0076 0.18 +1.7 73,411 - BT0076 0.18 -1.1 73,412 - BT0076 0.18 -2.6 73,412 - BT0076 0.18 +0.7 73,412 - BT0076 0.18 -0.1 73,571 - BT0076 0.31 -0.3 73,629 - BT0076 0.36 -3.4 73,629 - BT0076 0.36 +0.7 73,630 + BT0076 0.36 -2.3 73,631 - BT0076 0.36 +1.5 73,692 - BT0076 0.41 -1.3 73,692 - BT0076 0.41 -1.1 73,703 - BT0076 0.42 -0.9 73,721 - BT0076 0.44 -2.1
Or see this region's nucleotide sequence