Experiment: MoYLS4 with Neomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2532 and DvMF_2533 are separated by 323 nucleotides DvMF_2533 and DvMF_2534 are separated by 7 nucleotides DvMF_2534 and DvMF_2535 are separated by 113 nucleotides
DvMF_2532: DvMF_2532 - cytochrome c class III (RefSeq), at 3,194,419 to 3,196,350
_2532
DvMF_2533: DvMF_2533 - formate dehydrogenase, alpha subunit (RefSeq), at 3,196,674 to 3,199,706
_2533
DvMF_2534: DvMF_2534 - formate dehydrogenase beta subunit (RefSeq), at 3,199,714 to 3,200,466
_2534
DvMF_2535: DvMF_2535 - Methyltransferase type 11 (RefSeq), at 3,200,580 to 3,201,257
_2535
Position (kb)
3196
3197
3198
3199
3200 Strain fitness (log2 ratio)
-1
0
1 at 3196.009 kb on - strand, within DvMF_2532 at 3196.035 kb on + strand, within DvMF_2532 at 3196.152 kb on - strand, within DvMF_2532 at 3196.296 kb on + strand at 3196.361 kb on - strand at 3196.445 kb on - strand at 3196.761 kb on - strand at 3196.761 kb on - strand at 3196.821 kb on - strand at 3196.853 kb on + strand at 3197.160 kb on - strand, within DvMF_2533 at 3197.193 kb on + strand, within DvMF_2533 at 3197.392 kb on + strand, within DvMF_2533 at 3197.418 kb on - strand, within DvMF_2533 at 3197.645 kb on + strand, within DvMF_2533 at 3197.713 kb on + strand, within DvMF_2533 at 3197.753 kb on + strand, within DvMF_2533 at 3197.881 kb on + strand, within DvMF_2533 at 3197.900 kb on - strand, within DvMF_2533 at 3198.073 kb on - strand, within DvMF_2533 at 3198.214 kb on - strand, within DvMF_2533 at 3198.224 kb on - strand, within DvMF_2533 at 3198.396 kb on + strand, within DvMF_2533 at 3198.500 kb on - strand, within DvMF_2533 at 3198.635 kb on + strand, within DvMF_2533 at 3198.635 kb on + strand, within DvMF_2533 at 3198.643 kb on - strand, within DvMF_2533 at 3198.806 kb on - strand, within DvMF_2533 at 3198.886 kb on - strand, within DvMF_2533 at 3198.913 kb on + strand, within DvMF_2533 at 3199.001 kb on + strand, within DvMF_2533 at 3199.011 kb on + strand, within DvMF_2533 at 3199.220 kb on - strand, within DvMF_2533 at 3199.248 kb on + strand, within DvMF_2533 at 3199.248 kb on + strand, within DvMF_2533 at 3199.249 kb on - strand, within DvMF_2533 at 3199.256 kb on - strand, within DvMF_2533 at 3199.256 kb on - strand, within DvMF_2533 at 3199.268 kb on + strand, within DvMF_2533 at 3199.302 kb on - strand, within DvMF_2533 at 3199.419 kb on + strand at 3199.701 kb on + strand at 3199.755 kb on - strand at 3199.878 kb on + strand, within DvMF_2534 at 3199.900 kb on + strand, within DvMF_2534 at 3199.952 kb on + strand, within DvMF_2534 at 3199.960 kb on - strand, within DvMF_2534 at 3200.340 kb on - strand, within DvMF_2534 at 3200.461 kb on + strand at 3200.461 kb on + strand at 3200.461 kb on + strand at 3200.469 kb on - strand at 3200.469 kb on - strand at 3200.498 kb on - strand at 3200.577 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Neomycin 0.06 mg/ml remove 3,196,009 - DvMF_2532 0.82 -0.9 3,196,035 + DvMF_2532 0.84 -0.3 3,196,152 - DvMF_2532 0.90 -0.2 3,196,296 + -0.6 3,196,361 - +0.1 3,196,445 - -1.1 3,196,761 - -0.5 3,196,761 - -0.1 3,196,821 - -0.5 3,196,853 + -0.8 3,197,160 - DvMF_2533 0.16 +0.0 3,197,193 + DvMF_2533 0.17 -0.0 3,197,392 + DvMF_2533 0.24 -0.8 3,197,418 - DvMF_2533 0.25 -1.0 3,197,645 + DvMF_2533 0.32 -0.3 3,197,713 + DvMF_2533 0.34 -0.3 3,197,753 + DvMF_2533 0.36 -0.7 3,197,881 + DvMF_2533 0.40 -1.1 3,197,900 - DvMF_2533 0.40 -0.2 3,198,073 - DvMF_2533 0.46 -0.7 3,198,214 - DvMF_2533 0.51 -0.3 3,198,224 - DvMF_2533 0.51 -0.4 3,198,396 + DvMF_2533 0.57 -0.5 3,198,500 - DvMF_2533 0.60 -0.8 3,198,635 + DvMF_2533 0.65 -0.6 3,198,635 + DvMF_2533 0.65 -0.3 3,198,643 - DvMF_2533 0.65 -1.0 3,198,806 - DvMF_2533 0.70 -0.4 3,198,886 - DvMF_2533 0.73 +0.1 3,198,913 + DvMF_2533 0.74 +0.6 3,199,001 + DvMF_2533 0.77 -0.0 3,199,011 + DvMF_2533 0.77 -0.2 3,199,220 - DvMF_2533 0.84 -1.2 3,199,248 + DvMF_2533 0.85 -0.3 3,199,248 + DvMF_2533 0.85 -0.4 3,199,249 - DvMF_2533 0.85 +0.1 3,199,256 - DvMF_2533 0.85 -1.2 3,199,256 - DvMF_2533 0.85 -0.3 3,199,268 + DvMF_2533 0.86 -0.3 3,199,302 - DvMF_2533 0.87 -0.7 3,199,419 + +0.0 3,199,701 + +0.5 3,199,755 - -0.4 3,199,878 + DvMF_2534 0.22 -0.2 3,199,900 + DvMF_2534 0.25 -0.3 3,199,952 + DvMF_2534 0.32 +0.1 3,199,960 - DvMF_2534 0.33 -0.1 3,200,340 - DvMF_2534 0.83 -0.2 3,200,461 + +0.3 3,200,461 + -0.3 3,200,461 + +1.2 3,200,469 - -0.6 3,200,469 - -0.4 3,200,498 - -0.9 3,200,577 + -0.6
Or see this region's nucleotide sequence