Experiment: MoYLS4 with Neomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2056 and DvMF_2057 overlap by 1 nucleotides DvMF_2057 and DvMF_2058 are separated by 210 nucleotides DvMF_2058 and DvMF_2059 are separated by 281 nucleotides DvMF_2059 and DvMF_2060 are separated by 377 nucleotides
DvMF_2056: DvMF_2056 - thiamine pyrophosphate protein domain protein TPP-binding (RefSeq), at 2,548,364 to 2,549,155
_2056
DvMF_2057: DvMF_2057 - pyruvate ferredoxin/flavodoxin oxidoreductase (RefSeq), at 2,549,155 to 2,549,697
_2057
DvMF_2058: DvMF_2058 - putative superfamily I DNA and RNA helicase protein (RefSeq), at 2,549,908 to 2,555,493
_2058
DvMF_2059: DvMF_2059 - hypothetical protein (RefSeq), at 2,555,775 to 2,556,065
_2059
DvMF_2060: DvMF_2060 - small multidrug resistance protein (RefSeq), at 2,556,443 to 2,556,850
_2060
Position (kb)
2549
2550
2551
2552
2553
2554
2555
2556 Strain fitness (log2 ratio)
-2
-1
0
1 at 2548.987 kb on - strand, within DvMF_2056 at 2549.221 kb on + strand, within DvMF_2057 at 2549.409 kb on - strand, within DvMF_2057 at 2549.419 kb on - strand, within DvMF_2057 at 2549.434 kb on + strand, within DvMF_2057 at 2549.434 kb on + strand, within DvMF_2057 at 2549.451 kb on + strand, within DvMF_2057 at 2549.523 kb on + strand, within DvMF_2057 at 2550.018 kb on + strand at 2550.300 kb on - strand at 2550.300 kb on - strand at 2550.604 kb on + strand, within DvMF_2058 at 2550.794 kb on + strand, within DvMF_2058 at 2550.838 kb on - strand, within DvMF_2058 at 2550.907 kb on + strand, within DvMF_2058 at 2550.955 kb on + strand, within DvMF_2058 at 2551.317 kb on - strand, within DvMF_2058 at 2551.484 kb on - strand, within DvMF_2058 at 2552.121 kb on + strand, within DvMF_2058 at 2552.128 kb on - strand, within DvMF_2058 at 2552.268 kb on + strand, within DvMF_2058 at 2552.327 kb on - strand, within DvMF_2058 at 2552.327 kb on - strand, within DvMF_2058 at 2552.547 kb on + strand, within DvMF_2058 at 2552.605 kb on + strand, within DvMF_2058 at 2552.857 kb on - strand, within DvMF_2058 at 2552.953 kb on + strand, within DvMF_2058 at 2553.149 kb on - strand, within DvMF_2058 at 2553.290 kb on + strand, within DvMF_2058 at 2553.300 kb on + strand, within DvMF_2058 at 2553.300 kb on + strand, within DvMF_2058 at 2553.307 kb on + strand, within DvMF_2058 at 2553.308 kb on - strand, within DvMF_2058 at 2553.465 kb on + strand, within DvMF_2058 at 2553.473 kb on - strand, within DvMF_2058 at 2553.483 kb on - strand, within DvMF_2058 at 2553.715 kb on + strand, within DvMF_2058 at 2554.028 kb on + strand, within DvMF_2058 at 2554.195 kb on + strand, within DvMF_2058 at 2554.203 kb on - strand, within DvMF_2058 at 2554.209 kb on - strand, within DvMF_2058 at 2554.325 kb on - strand, within DvMF_2058 at 2554.486 kb on - strand, within DvMF_2058 at 2554.501 kb on + strand, within DvMF_2058 at 2554.504 kb on - strand, within DvMF_2058 at 2554.645 kb on - strand, within DvMF_2058 at 2554.650 kb on - strand, within DvMF_2058 at 2555.011 kb on + strand at 2555.117 kb on + strand at 2555.217 kb on + strand at 2555.316 kb on - strand at 2555.708 kb on + strand at 2555.708 kb on + strand at 2555.716 kb on - strand at 2555.716 kb on - strand at 2555.808 kb on - strand, within DvMF_2059 at 2556.033 kb on + strand, within DvMF_2059 at 2556.051 kb on - strand at 2556.233 kb on - strand at 2556.462 kb on + strand at 2556.470 kb on - strand at 2556.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Neomycin 0.06 mg/ml remove 2,548,987 - DvMF_2056 0.79 -2.1 2,549,221 + DvMF_2057 0.12 -2.3 2,549,409 - DvMF_2057 0.47 -0.5 2,549,419 - DvMF_2057 0.49 -1.5 2,549,434 + DvMF_2057 0.51 -2.1 2,549,434 + DvMF_2057 0.51 -1.1 2,549,451 + DvMF_2057 0.55 -1.9 2,549,523 + DvMF_2057 0.68 -1.1 2,550,018 + +0.4 2,550,300 - -0.2 2,550,300 - -0.2 2,550,604 + DvMF_2058 0.12 -0.6 2,550,794 + DvMF_2058 0.16 +1.1 2,550,838 - DvMF_2058 0.17 -0.2 2,550,907 + DvMF_2058 0.18 +0.0 2,550,955 + DvMF_2058 0.19 -0.8 2,551,317 - DvMF_2058 0.25 -0.0 2,551,484 - DvMF_2058 0.28 -0.4 2,552,121 + DvMF_2058 0.40 +0.3 2,552,128 - DvMF_2058 0.40 +0.3 2,552,268 + DvMF_2058 0.42 -0.4 2,552,327 - DvMF_2058 0.43 -0.1 2,552,327 - DvMF_2058 0.43 -0.3 2,552,547 + DvMF_2058 0.47 -0.0 2,552,605 + DvMF_2058 0.48 -0.1 2,552,857 - DvMF_2058 0.53 -0.0 2,552,953 + DvMF_2058 0.55 -0.4 2,553,149 - DvMF_2058 0.58 -0.3 2,553,290 + DvMF_2058 0.61 -0.3 2,553,300 + DvMF_2058 0.61 -0.1 2,553,300 + DvMF_2058 0.61 -0.4 2,553,307 + DvMF_2058 0.61 -0.9 2,553,308 - DvMF_2058 0.61 -0.5 2,553,465 + DvMF_2058 0.64 -0.4 2,553,473 - DvMF_2058 0.64 -0.6 2,553,483 - DvMF_2058 0.64 -0.5 2,553,715 + DvMF_2058 0.68 -0.1 2,554,028 + DvMF_2058 0.74 -0.6 2,554,195 + DvMF_2058 0.77 -0.5 2,554,203 - DvMF_2058 0.77 +0.1 2,554,209 - DvMF_2058 0.77 -0.2 2,554,325 - DvMF_2058 0.79 -0.4 2,554,486 - DvMF_2058 0.82 -0.5 2,554,501 + DvMF_2058 0.82 +0.2 2,554,504 - DvMF_2058 0.82 -0.6 2,554,645 - DvMF_2058 0.85 -0.2 2,554,650 - DvMF_2058 0.85 -0.2 2,555,011 + -1.1 2,555,117 + -0.1 2,555,217 + -0.3 2,555,316 - -0.6 2,555,708 + -0.5 2,555,708 + -0.7 2,555,716 - +0.3 2,555,716 - +0.1 2,555,808 - DvMF_2059 0.11 +0.1 2,556,033 + DvMF_2059 0.89 -0.4 2,556,051 - -0.4 2,556,233 - -0.4 2,556,462 + -0.4 2,556,470 - -0.1 2,556,470 - -0.4
Or see this region's nucleotide sequence