Experiment: MoYLS4 with Neomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0686 and DvMF_0687 are separated by 11 nucleotides DvMF_0687 and DvMF_0688 are separated by 10 nucleotides DvMF_0688 and DvMF_0689 are separated by 60 nucleotides DvMF_0689 and DvMF_0690 overlap by 1 nucleotides
DvMF_0686: DvMF_0686 - secretion ATPase, PEP-CTERM locus subfamily (RefSeq), at 827,628 to 828,815
_0686
DvMF_0687: DvMF_0687 - exopolysaccharide/PEPCTERM locus tyrosine autokinase (RefSeq), at 828,827 to 829,708
_0687
DvMF_0688: DvMF_0688 - polysaccharide chain length determinant protein, PEP-CTERM locus subfamily (RefSeq), at 829,719 to 831,161
_0688
DvMF_0689: DvMF_0689 - polysaccharide export protein, PEP-CTERM sytem-associated (RefSeq), at 831,222 to 832,028
_0689
DvMF_0690: DvMF_0690 - sugar transferase, PEP-CTERM system associated (RefSeq), at 832,028 to 833,359
_0690
Position (kb) 829
830
831
832 Strain fitness (log2 ratio) -1
0
1
2 at 828.808 kb on + strand at 828.935 kb on + strand, within DvMF_0687 at 829.158 kb on - strand, within DvMF_0687 at 829.343 kb on + strand, within DvMF_0687 at 829.348 kb on + strand, within DvMF_0687 at 829.351 kb on - strand, within DvMF_0687 at 829.538 kb on - strand, within DvMF_0687 at 829.717 kb on + strand at 829.725 kb on - strand at 829.725 kb on - strand at 829.725 kb on - strand at 829.804 kb on - strand at 829.815 kb on - strand at 829.820 kb on + strand at 829.863 kb on - strand at 829.985 kb on - strand, within DvMF_0688 at 830.273 kb on + strand, within DvMF_0688 at 830.488 kb on + strand, within DvMF_0688 at 831.063 kb on - strand at 831.083 kb on + strand at 831.156 kb on - strand at 831.210 kb on + strand at 831.220 kb on + strand at 831.228 kb on - strand at 831.454 kb on - strand, within DvMF_0689 at 831.521 kb on - strand, within DvMF_0689 at 831.544 kb on + strand, within DvMF_0689 at 831.672 kb on - strand, within DvMF_0689 at 831.673 kb on - strand, within DvMF_0689
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Neomycin 0.06 mg/ml remove 828,808 + -0.1 828,935 + DvMF_0687 0.12 -0.4 829,158 - DvMF_0687 0.38 -0.5 829,343 + DvMF_0687 0.59 -0.3 829,348 + DvMF_0687 0.59 +0.2 829,351 - DvMF_0687 0.59 +0.6 829,538 - DvMF_0687 0.81 -0.4 829,717 + -0.9 829,725 - -0.5 829,725 - -0.4 829,725 - -0.4 829,804 - -0.1 829,815 - -0.6 829,820 + -0.4 829,863 - +0.4 829,985 - DvMF_0688 0.18 -0.7 830,273 + DvMF_0688 0.38 -0.4 830,488 + DvMF_0688 0.53 -1.0 831,063 - -0.4 831,083 + -1.2 831,156 - -0.4 831,210 + -0.2 831,220 + -0.5 831,228 - -0.1 831,454 - DvMF_0689 0.29 -0.1 831,521 - DvMF_0689 0.37 -0.3 831,544 + DvMF_0689 0.40 +1.9 831,672 - DvMF_0689 0.56 -1.1 831,673 - DvMF_0689 0.56 -0.2
Or see this region's nucleotide sequence