Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3122 and Shew_3123 are separated by 132 nucleotides Shew_3123 and Shew_3124 are separated by 27 nucleotides
Shew_3122: Shew_3122 - PepSY-associated TM helix domain-containing protein (RefSeq), at 3,709,997 to 3,710,797
_3122
Shew_3123: Shew_3123 - penicillin-binding protein 1B (RefSeq), at 3,710,930 to 3,713,299
_3123
Shew_3124: Shew_3124 - ATP-dependent helicase HrpB (RefSeq), at 3,713,327 to 3,715,858
_3124
Position (kb)
3710
3711
3712
3713
3714 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3710.460 kb on - strand, within Shew_3122 at 3710.809 kb on + strand at 3710.809 kb on + strand at 3710.817 kb on - strand at 3710.893 kb on - strand at 3712.043 kb on - strand, within Shew_3123 at 3712.066 kb on + strand, within Shew_3123 at 3712.164 kb on - strand, within Shew_3123 at 3712.400 kb on + strand, within Shew_3123 at 3712.565 kb on + strand, within Shew_3123 at 3712.565 kb on + strand, within Shew_3123 at 3712.573 kb on - strand, within Shew_3123 at 3712.573 kb on - strand, within Shew_3123 at 3712.573 kb on - strand, within Shew_3123 at 3712.573 kb on - strand, within Shew_3123 at 3712.573 kb on - strand, within Shew_3123 at 3712.597 kb on - strand, within Shew_3123 at 3712.995 kb on - strand, within Shew_3123 at 3712.997 kb on + strand, within Shew_3123 at 3713.404 kb on + strand at 3713.702 kb on - strand, within Shew_3124 at 3713.733 kb on + strand, within Shew_3124
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,710,460 - Shew_3122 0.58 +0.4 3,710,809 + +0.6 3,710,809 + -0.3 3,710,817 - -0.1 3,710,893 - +0.2 3,712,043 - Shew_3123 0.47 -1.0 3,712,066 + Shew_3123 0.48 -1.0 3,712,164 - Shew_3123 0.52 -1.0 3,712,400 + Shew_3123 0.62 +0.3 3,712,565 + Shew_3123 0.69 -1.6 3,712,565 + Shew_3123 0.69 -3.3 3,712,573 - Shew_3123 0.69 -1.3 3,712,573 - Shew_3123 0.69 +0.2 3,712,573 - Shew_3123 0.69 +1.0 3,712,573 - Shew_3123 0.69 -1.6 3,712,573 - Shew_3123 0.69 -1.0 3,712,597 - Shew_3123 0.70 -0.9 3,712,995 - Shew_3123 0.87 -0.4 3,712,997 + Shew_3123 0.87 -0.5 3,713,404 + -0.9 3,713,702 - Shew_3124 0.15 +0.4 3,713,733 + Shew_3124 0.16 +0.3
Or see this region's nucleotide sequence