Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2897 and Shew_2898 are separated by 152 nucleotides Shew_2898 and Shew_2899 are separated by 3 nucleotides Shew_2899 and Shew_2900 overlap by 14 nucleotides
Shew_2897: Shew_2897 - LysR family transcriptional regulator (RefSeq), at 3,435,587 to 3,436,471
_2897
Shew_2898: Shew_2898 - hypothetical protein (RefSeq), at 3,436,624 to 3,436,983
_2898
Shew_2899: Shew_2899 - glutathione synthetase (RefSeq), at 3,436,987 to 3,438,021
_2899
Shew_2900: Shew_2900 - hypothetical protein (RefSeq), at 3,438,008 to 3,439,999
_2900
Position (kb)
3436
3437
3438
3439 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3436.206 kb on + strand, within Shew_2897 at 3436.243 kb on + strand, within Shew_2897 at 3436.243 kb on + strand, within Shew_2897 at 3436.243 kb on + strand, within Shew_2897 at 3436.251 kb on - strand, within Shew_2897 at 3436.446 kb on - strand at 3436.914 kb on - strand, within Shew_2898 at 3437.086 kb on - strand at 3437.696 kb on + strand, within Shew_2899 at 3437.789 kb on + strand, within Shew_2899 at 3437.879 kb on + strand, within Shew_2899 at 3437.976 kb on + strand at 3438.151 kb on - strand at 3438.609 kb on - strand, within Shew_2900 at 3438.690 kb on + strand, within Shew_2900
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,436,206 + Shew_2897 0.70 -2.6 3,436,243 + Shew_2897 0.74 -0.3 3,436,243 + Shew_2897 0.74 -0.2 3,436,243 + Shew_2897 0.74 -0.5 3,436,251 - Shew_2897 0.75 +0.7 3,436,446 - +0.0 3,436,914 - Shew_2898 0.81 -3.2 3,437,086 - -0.4 3,437,696 + Shew_2899 0.69 -0.4 3,437,789 + Shew_2899 0.77 -0.9 3,437,879 + Shew_2899 0.86 -1.8 3,437,976 + -1.0 3,438,151 - +0.4 3,438,609 - Shew_2900 0.30 -1.9 3,438,690 + Shew_2900 0.34 -0.9
Or see this region's nucleotide sequence