Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2183 and Shew_2184 are separated by 126 nucleotides Shew_2184 and Shew_2185 are separated by 24 nucleotides Shew_2185 and Shew_2186 are separated by 2 nucleotides Shew_2186 and Shew_2187 are separated by 109 nucleotides
Shew_2183: Shew_2183 - hypothetical protein (RefSeq), at 2,543,261 to 2,543,542
_2183
Shew_2184: Shew_2184 - septum site-determining protein MinC (RefSeq), at 2,543,669 to 2,544,334
_2184
Shew_2185: Shew_2185 - septum site-determining protein MinD (RefSeq), at 2,544,359 to 2,545,168
_2185
Shew_2186: Shew_2186 - cell division topological specificity factor MinE (RefSeq), at 2,545,171 to 2,545,431
_2186
Shew_2187: Shew_2187 - ribonuclease D (RefSeq), at 2,545,541 to 2,546,647
_2187
Position (kb)
2544
2545
2546 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2543.522 kb on + strand at 2543.522 kb on + strand at 2543.522 kb on + strand at 2543.522 kb on + strand at 2543.530 kb on - strand at 2543.530 kb on - strand at 2543.591 kb on + strand at 2543.597 kb on - strand at 2543.599 kb on - strand at 2543.631 kb on + strand at 2543.733 kb on - strand at 2543.943 kb on - strand, within Shew_2184 at 2543.943 kb on - strand, within Shew_2184 at 2544.294 kb on - strand at 2544.398 kb on - strand at 2544.527 kb on + strand, within Shew_2185 at 2544.872 kb on - strand, within Shew_2185 at 2544.872 kb on - strand, within Shew_2185 at 2545.066 kb on + strand, within Shew_2185 at 2545.834 kb on + strand, within Shew_2187 at 2546.011 kb on + strand, within Shew_2187 at 2546.152 kb on + strand, within Shew_2187
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 2,543,522 + -0.1 2,543,522 + +0.5 2,543,522 + -0.7 2,543,522 + -0.5 2,543,530 - -0.2 2,543,530 - -0.2 2,543,591 + -1.4 2,543,597 - +0.1 2,543,599 - -3.3 2,543,631 + -0.5 2,543,733 - -1.1 2,543,943 - Shew_2184 0.41 -1.0 2,543,943 - Shew_2184 0.41 -2.6 2,544,294 - -1.7 2,544,398 - -0.7 2,544,527 + Shew_2185 0.21 -1.7 2,544,872 - Shew_2185 0.63 -1.5 2,544,872 - Shew_2185 0.63 -0.7 2,545,066 + Shew_2185 0.87 -0.6 2,545,834 + Shew_2187 0.26 -0.3 2,546,011 + Shew_2187 0.42 +0.8 2,546,152 + Shew_2187 0.55 -0.1
Or see this region's nucleotide sequence