Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1511 and Shew_1512 are separated by 260 nucleotides Shew_1512 and Shew_1513 overlap by 1 nucleotides Shew_1513 and upp are separated by 211 nucleotides upp and Shew_1515 are separated by 94 nucleotides
Shew_1511: Shew_1511 - UMP phosphatase (RefSeq), at 1,734,073 to 1,734,831
_1511
Shew_1512: Shew_1512 - phosphoribosylglycinamide formyltransferase (RefSeq), at 1,735,092 to 1,735,736
_1512
Shew_1513: Shew_1513 - phosphoribosylaminoimidazole synthetase (RefSeq), at 1,735,736 to 1,736,773
_1513
Shew_1514: upp - uracil phosphoribosyltransferase (RefSeq), at 1,736,985 to 1,737,611
upp
Shew_1515: Shew_1515 - Na+/H+ antiporter NhaC (RefSeq), at 1,737,706 to 1,739,025
_1515
Position (kb) 1735
1736
1737 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 1734.939 kb on - strand at 1735.002 kb on - strand at 1735.172 kb on + strand, within Shew_1512 at 1735.666 kb on - strand, within Shew_1512 at 1736.251 kb on + strand, within Shew_1513 at 1737.150 kb on + strand, within upp at 1737.570 kb on + strand at 1737.601 kb on + strand at 1737.699 kb on + strand at 1737.759 kb on + strand at 1737.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 1,734,939 - -0.2 1,735,002 - -0.7 1,735,172 + Shew_1512 0.12 +1.8 1,735,666 - Shew_1512 0.89 +1.8 1,736,251 + Shew_1513 0.50 +3.8 1,737,150 + upp Shew_1514 0.26 -1.7 1,737,570 + -1.2 1,737,601 + -1.0 1,737,699 + -0.5 1,737,759 + +0.5 1,737,767 - +1.8
Or see this region's nucleotide sequence