Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt engA and Shew_1295 are separated by 190 nucleotides Shew_1295 and xseA are separated by 231 nucleotides xseA and Shew_1297 are separated by 119 nucleotides
Shew_1294: engA - GTP-binding protein EngA (RefSeq), at 1,494,671 to 1,496,140
engA
Shew_1295: Shew_1295 - hypothetical protein (RefSeq), at 1,496,331 to 1,496,864
_1295
Shew_1296: xseA - exodeoxyribonuclease VII large subunit (RefSeq), at 1,497,096 to 1,498,424
xseA
Shew_1297: Shew_1297 - inositol-5-monophosphate dehydrogenase (RefSeq), at 1,498,544 to 1,500,010
_1297
Position (kb)
1497
1498
1499 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1496.197 kb on - strand at 1496.314 kb on + strand at 1496.578 kb on - strand, within Shew_1295 at 1496.784 kb on - strand, within Shew_1295 at 1496.791 kb on - strand, within Shew_1295 at 1497.011 kb on + strand at 1497.101 kb on - strand at 1497.131 kb on + strand at 1497.139 kb on - strand at 1497.161 kb on + strand at 1497.174 kb on + strand at 1497.203 kb on - strand at 1497.294 kb on - strand, within xseA at 1497.315 kb on - strand, within xseA at 1497.388 kb on - strand, within xseA at 1497.428 kb on + strand, within xseA at 1497.718 kb on - strand, within xseA at 1497.755 kb on - strand, within xseA at 1497.762 kb on + strand, within xseA at 1497.964 kb on + strand, within xseA at 1498.208 kb on + strand, within xseA at 1498.347 kb on - strand at 1498.349 kb on + strand at 1498.487 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 1,496,197 - -0.1 1,496,314 + +0.0 1,496,578 - Shew_1295 0.46 -0.2 1,496,784 - Shew_1295 0.85 -0.9 1,496,791 - Shew_1295 0.86 -1.9 1,497,011 + -0.1 1,497,101 - -0.6 1,497,131 + -0.1 1,497,139 - -0.8 1,497,161 + -0.5 1,497,174 + -2.9 1,497,203 - -2.4 1,497,294 - xseA Shew_1296 0.15 -1.1 1,497,315 - xseA Shew_1296 0.16 -1.2 1,497,388 - xseA Shew_1296 0.22 -0.8 1,497,428 + xseA Shew_1296 0.25 -0.8 1,497,718 - xseA Shew_1296 0.47 -0.7 1,497,755 - xseA Shew_1296 0.50 -0.9 1,497,762 + xseA Shew_1296 0.50 -1.1 1,497,964 + xseA Shew_1296 0.65 -1.2 1,498,208 + xseA Shew_1296 0.84 -0.9 1,498,347 - -0.6 1,498,349 + -0.7 1,498,487 + +0.8
Or see this region's nucleotide sequence